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An artificial chromosome for data storage
DNA digital storage provides an alternative for information storage with high density and long-term stability. Here, we report the de novo design and synthesis of an artificial chromosome that encodes two pictures and a video clip. The encoding paradigm utilizing the superposition of sparsified erro...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288405/ https://www.ncbi.nlm.nih.gov/pubmed/34691648 http://dx.doi.org/10.1093/nsr/nwab028 |
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author | Chen, Weigang Han, Mingzhe Zhou, Jianting Ge, Qi Wang, Panpan Zhang, Xinchen Zhu, Siyu Song, Lifu Yuan, Yingjin |
author_facet | Chen, Weigang Han, Mingzhe Zhou, Jianting Ge, Qi Wang, Panpan Zhang, Xinchen Zhu, Siyu Song, Lifu Yuan, Yingjin |
author_sort | Chen, Weigang |
collection | PubMed |
description | DNA digital storage provides an alternative for information storage with high density and long-term stability. Here, we report the de novo design and synthesis of an artificial chromosome that encodes two pictures and a video clip. The encoding paradigm utilizing the superposition of sparsified error correction codewords and pseudo-random sequences tolerates base insertions/deletions and is well suited to error-prone nanopore sequencing for data retrieval. The entire 254 kb sequence was 95.27% occupied by encoded data. The Transformation-Associated Recombination method was used in the construction of this chromosome from DNA fragments and necessary autonomous replication sequences. The stability was demonstrated by transmitting the data-carrying chromosome to the 100th generation. This study demonstrates a data storage method using encoded artificial chromosomes via in vivo assembly for write-once and stable replication for multiple retrievals, similar to a compact disc, with potential in economically massive data distribution. |
format | Online Article Text |
id | pubmed-8288405 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-82884052021-10-21 An artificial chromosome for data storage Chen, Weigang Han, Mingzhe Zhou, Jianting Ge, Qi Wang, Panpan Zhang, Xinchen Zhu, Siyu Song, Lifu Yuan, Yingjin Natl Sci Rev Information Science DNA digital storage provides an alternative for information storage with high density and long-term stability. Here, we report the de novo design and synthesis of an artificial chromosome that encodes two pictures and a video clip. The encoding paradigm utilizing the superposition of sparsified error correction codewords and pseudo-random sequences tolerates base insertions/deletions and is well suited to error-prone nanopore sequencing for data retrieval. The entire 254 kb sequence was 95.27% occupied by encoded data. The Transformation-Associated Recombination method was used in the construction of this chromosome from DNA fragments and necessary autonomous replication sequences. The stability was demonstrated by transmitting the data-carrying chromosome to the 100th generation. This study demonstrates a data storage method using encoded artificial chromosomes via in vivo assembly for write-once and stable replication for multiple retrievals, similar to a compact disc, with potential in economically massive data distribution. Oxford University Press 2021-02-12 /pmc/articles/PMC8288405/ /pubmed/34691648 http://dx.doi.org/10.1093/nsr/nwab028 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Information Science Chen, Weigang Han, Mingzhe Zhou, Jianting Ge, Qi Wang, Panpan Zhang, Xinchen Zhu, Siyu Song, Lifu Yuan, Yingjin An artificial chromosome for data storage |
title | An artificial chromosome for data storage |
title_full | An artificial chromosome for data storage |
title_fullStr | An artificial chromosome for data storage |
title_full_unstemmed | An artificial chromosome for data storage |
title_short | An artificial chromosome for data storage |
title_sort | artificial chromosome for data storage |
topic | Information Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288405/ https://www.ncbi.nlm.nih.gov/pubmed/34691648 http://dx.doi.org/10.1093/nsr/nwab028 |
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