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Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells
Human cerebral organoid (CO) is a three-dimensional (3D) cell culture system that recapitulates the developing human brain. While CO has proved an invaluable tool for studying neurological disorders in a more clinically relevant matter, there have still been several shortcomings including CO variabi...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288463/ https://www.ncbi.nlm.nih.gov/pubmed/34290591 http://dx.doi.org/10.3389/fncel.2021.682272 |
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author | Bodnar, Brittany Zhang, Yongang Liu, Jinbiao Lin, Yuan Wang, Peng Wei, Zhengyu Saribas, Sami Zhu, Yuanjun Li, Fang Wang, Xu Yang, Wenli Li, Qingsheng Ho, Wen-Zhe Hu, Wenhui |
author_facet | Bodnar, Brittany Zhang, Yongang Liu, Jinbiao Lin, Yuan Wang, Peng Wei, Zhengyu Saribas, Sami Zhu, Yuanjun Li, Fang Wang, Xu Yang, Wenli Li, Qingsheng Ho, Wen-Zhe Hu, Wenhui |
author_sort | Bodnar, Brittany |
collection | PubMed |
description | Human cerebral organoid (CO) is a three-dimensional (3D) cell culture system that recapitulates the developing human brain. While CO has proved an invaluable tool for studying neurological disorders in a more clinically relevant matter, there have still been several shortcomings including CO variability and reproducibility as well as lack of or underrepresentation of certain cell types typically found in the brain. As the technology to generate COs has continued to improve, more efficient and streamlined protocols have addressed some of these issues. Here we present a novel scalable and simplified system to generate microglia-containing CO (MCO). We characterize the cell types and dynamic development of MCOs and validate that these MCOs harbor microglia, astrocytes, neurons, and neural stem/progenitor cells, maturing in a manner that reflects human brain development. We introduce a novel technique for the generation of embryoid bodies (EBs) directly from induced pluripotent stem cells (iPSCs) that involves simplified steps of transitioning directly from 3D cultures as well as orbital shaking culture in a standard 6-well culture plate. This allows for the generation of MCOs with an easy-to-use system that is affordable and accessible by any general lab. |
format | Online Article Text |
id | pubmed-8288463 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82884632021-07-20 Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells Bodnar, Brittany Zhang, Yongang Liu, Jinbiao Lin, Yuan Wang, Peng Wei, Zhengyu Saribas, Sami Zhu, Yuanjun Li, Fang Wang, Xu Yang, Wenli Li, Qingsheng Ho, Wen-Zhe Hu, Wenhui Front Cell Neurosci Cellular Neuroscience Human cerebral organoid (CO) is a three-dimensional (3D) cell culture system that recapitulates the developing human brain. While CO has proved an invaluable tool for studying neurological disorders in a more clinically relevant matter, there have still been several shortcomings including CO variability and reproducibility as well as lack of or underrepresentation of certain cell types typically found in the brain. As the technology to generate COs has continued to improve, more efficient and streamlined protocols have addressed some of these issues. Here we present a novel scalable and simplified system to generate microglia-containing CO (MCO). We characterize the cell types and dynamic development of MCOs and validate that these MCOs harbor microglia, astrocytes, neurons, and neural stem/progenitor cells, maturing in a manner that reflects human brain development. We introduce a novel technique for the generation of embryoid bodies (EBs) directly from induced pluripotent stem cells (iPSCs) that involves simplified steps of transitioning directly from 3D cultures as well as orbital shaking culture in a standard 6-well culture plate. This allows for the generation of MCOs with an easy-to-use system that is affordable and accessible by any general lab. Frontiers Media S.A. 2021-07-05 /pmc/articles/PMC8288463/ /pubmed/34290591 http://dx.doi.org/10.3389/fncel.2021.682272 Text en Copyright © 2021 Bodnar, Zhang, Liu, Lin, Wang, Wei, Saribas, Zhu, Li, Wang, Yang, Li, Ho and Hu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular Neuroscience Bodnar, Brittany Zhang, Yongang Liu, Jinbiao Lin, Yuan Wang, Peng Wei, Zhengyu Saribas, Sami Zhu, Yuanjun Li, Fang Wang, Xu Yang, Wenli Li, Qingsheng Ho, Wen-Zhe Hu, Wenhui Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells |
title | Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells |
title_full | Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells |
title_fullStr | Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells |
title_full_unstemmed | Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells |
title_short | Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells |
title_sort | novel scalable and simplified system to generate microglia-containing cerebral organoids from human induced pluripotent stem cells |
topic | Cellular Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288463/ https://www.ncbi.nlm.nih.gov/pubmed/34290591 http://dx.doi.org/10.3389/fncel.2021.682272 |
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