Cargando…

Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells

Human cerebral organoid (CO) is a three-dimensional (3D) cell culture system that recapitulates the developing human brain. While CO has proved an invaluable tool for studying neurological disorders in a more clinically relevant matter, there have still been several shortcomings including CO variabi...

Descripción completa

Detalles Bibliográficos
Autores principales: Bodnar, Brittany, Zhang, Yongang, Liu, Jinbiao, Lin, Yuan, Wang, Peng, Wei, Zhengyu, Saribas, Sami, Zhu, Yuanjun, Li, Fang, Wang, Xu, Yang, Wenli, Li, Qingsheng, Ho, Wen-Zhe, Hu, Wenhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288463/
https://www.ncbi.nlm.nih.gov/pubmed/34290591
http://dx.doi.org/10.3389/fncel.2021.682272
_version_ 1783724092664315904
author Bodnar, Brittany
Zhang, Yongang
Liu, Jinbiao
Lin, Yuan
Wang, Peng
Wei, Zhengyu
Saribas, Sami
Zhu, Yuanjun
Li, Fang
Wang, Xu
Yang, Wenli
Li, Qingsheng
Ho, Wen-Zhe
Hu, Wenhui
author_facet Bodnar, Brittany
Zhang, Yongang
Liu, Jinbiao
Lin, Yuan
Wang, Peng
Wei, Zhengyu
Saribas, Sami
Zhu, Yuanjun
Li, Fang
Wang, Xu
Yang, Wenli
Li, Qingsheng
Ho, Wen-Zhe
Hu, Wenhui
author_sort Bodnar, Brittany
collection PubMed
description Human cerebral organoid (CO) is a three-dimensional (3D) cell culture system that recapitulates the developing human brain. While CO has proved an invaluable tool for studying neurological disorders in a more clinically relevant matter, there have still been several shortcomings including CO variability and reproducibility as well as lack of or underrepresentation of certain cell types typically found in the brain. As the technology to generate COs has continued to improve, more efficient and streamlined protocols have addressed some of these issues. Here we present a novel scalable and simplified system to generate microglia-containing CO (MCO). We characterize the cell types and dynamic development of MCOs and validate that these MCOs harbor microglia, astrocytes, neurons, and neural stem/progenitor cells, maturing in a manner that reflects human brain development. We introduce a novel technique for the generation of embryoid bodies (EBs) directly from induced pluripotent stem cells (iPSCs) that involves simplified steps of transitioning directly from 3D cultures as well as orbital shaking culture in a standard 6-well culture plate. This allows for the generation of MCOs with an easy-to-use system that is affordable and accessible by any general lab.
format Online
Article
Text
id pubmed-8288463
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82884632021-07-20 Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells Bodnar, Brittany Zhang, Yongang Liu, Jinbiao Lin, Yuan Wang, Peng Wei, Zhengyu Saribas, Sami Zhu, Yuanjun Li, Fang Wang, Xu Yang, Wenli Li, Qingsheng Ho, Wen-Zhe Hu, Wenhui Front Cell Neurosci Cellular Neuroscience Human cerebral organoid (CO) is a three-dimensional (3D) cell culture system that recapitulates the developing human brain. While CO has proved an invaluable tool for studying neurological disorders in a more clinically relevant matter, there have still been several shortcomings including CO variability and reproducibility as well as lack of or underrepresentation of certain cell types typically found in the brain. As the technology to generate COs has continued to improve, more efficient and streamlined protocols have addressed some of these issues. Here we present a novel scalable and simplified system to generate microglia-containing CO (MCO). We characterize the cell types and dynamic development of MCOs and validate that these MCOs harbor microglia, astrocytes, neurons, and neural stem/progenitor cells, maturing in a manner that reflects human brain development. We introduce a novel technique for the generation of embryoid bodies (EBs) directly from induced pluripotent stem cells (iPSCs) that involves simplified steps of transitioning directly from 3D cultures as well as orbital shaking culture in a standard 6-well culture plate. This allows for the generation of MCOs with an easy-to-use system that is affordable and accessible by any general lab. Frontiers Media S.A. 2021-07-05 /pmc/articles/PMC8288463/ /pubmed/34290591 http://dx.doi.org/10.3389/fncel.2021.682272 Text en Copyright © 2021 Bodnar, Zhang, Liu, Lin, Wang, Wei, Saribas, Zhu, Li, Wang, Yang, Li, Ho and Hu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular Neuroscience
Bodnar, Brittany
Zhang, Yongang
Liu, Jinbiao
Lin, Yuan
Wang, Peng
Wei, Zhengyu
Saribas, Sami
Zhu, Yuanjun
Li, Fang
Wang, Xu
Yang, Wenli
Li, Qingsheng
Ho, Wen-Zhe
Hu, Wenhui
Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells
title Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells
title_full Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells
title_fullStr Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells
title_full_unstemmed Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells
title_short Novel Scalable and Simplified System to Generate Microglia-Containing Cerebral Organoids From Human Induced Pluripotent Stem Cells
title_sort novel scalable and simplified system to generate microglia-containing cerebral organoids from human induced pluripotent stem cells
topic Cellular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288463/
https://www.ncbi.nlm.nih.gov/pubmed/34290591
http://dx.doi.org/10.3389/fncel.2021.682272
work_keys_str_mv AT bodnarbrittany novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT zhangyongang novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT liujinbiao novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT linyuan novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT wangpeng novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT weizhengyu novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT saribassami novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT zhuyuanjun novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT lifang novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT wangxu novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT yangwenli novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT liqingsheng novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT howenzhe novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells
AT huwenhui novelscalableandsimplifiedsystemtogeneratemicrogliacontainingcerebralorganoidsfromhumaninducedpluripotentstemcells