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Caveolin-1 promotes tumor cell proliferation and vasculogenic mimicry formation in human glioma

Vasculogenic mimicry (VM) plays an important role in human glioma progression and resistance to antiangiogenic therapy as a compensatory neovascularization mechanism in malignant tumors. Caveolin-1 (Cav-1) has been found to contribute to VM formation. However, it remains largely unknown whether Cav-...

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Autores principales: Chen, Wenli, Cheng, Xing, Wang, Xiaobo, Hu, Wenjie, Wang, Jinshan, Liao, Chuangxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289350/
https://www.ncbi.nlm.nih.gov/pubmed/34287575
http://dx.doi.org/10.1590/1414-431X2020e10653
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author Chen, Wenli
Cheng, Xing
Wang, Xiaobo
Hu, Wenjie
Wang, Jinshan
Liao, Chuangxin
author_facet Chen, Wenli
Cheng, Xing
Wang, Xiaobo
Hu, Wenjie
Wang, Jinshan
Liao, Chuangxin
author_sort Chen, Wenli
collection PubMed
description Vasculogenic mimicry (VM) plays an important role in human glioma progression and resistance to antiangiogenic therapy as a compensatory neovascularization mechanism in malignant tumors. Caveolin-1 (Cav-1) has been found to contribute to VM formation. However, it remains largely unknown whether Cav-1 expression correlates with VM in glioma. In this study, we examined CAV-1 expression levels and VM in human glioma cell lines and in 94 human gliomas with different grades of malignancy, and present Cox proportional hazards regression. The molecular role of Cav-1 in glioma cells was investigated using quantitative polymerase chain reaction (qRT-PCR) assays, western blotting, CCK-8 assays, and tubule formation assays. Cav-1 expression and VM formation were positively correlated with each other and both were closely associated with glioma development and progression as evidenced by the presence of cystic tumor, shortened survival time, and advanced-stage glioma in glioma patients with Cav-1 overexpression/increased VM formation. Cav-1 promoted U251 glioma cell proliferation and VM formation in a Matrigel-based 3D culture model. VM-associated factors including hypoxia-inducible factor 1α (HIF-1α) and p-Akt was significantly elevated by Cav-1 overexpression but suppressed by siCav-1 in U251 cells. Collectively, our study identified Cav-1 as an important regulator of glioma cell proliferation and VM formation, contributing to glioma development and progression.
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spelling pubmed-82893502021-08-02 Caveolin-1 promotes tumor cell proliferation and vasculogenic mimicry formation in human glioma Chen, Wenli Cheng, Xing Wang, Xiaobo Hu, Wenjie Wang, Jinshan Liao, Chuangxin Braz J Med Biol Res Research Article Vasculogenic mimicry (VM) plays an important role in human glioma progression and resistance to antiangiogenic therapy as a compensatory neovascularization mechanism in malignant tumors. Caveolin-1 (Cav-1) has been found to contribute to VM formation. However, it remains largely unknown whether Cav-1 expression correlates with VM in glioma. In this study, we examined CAV-1 expression levels and VM in human glioma cell lines and in 94 human gliomas with different grades of malignancy, and present Cox proportional hazards regression. The molecular role of Cav-1 in glioma cells was investigated using quantitative polymerase chain reaction (qRT-PCR) assays, western blotting, CCK-8 assays, and tubule formation assays. Cav-1 expression and VM formation were positively correlated with each other and both were closely associated with glioma development and progression as evidenced by the presence of cystic tumor, shortened survival time, and advanced-stage glioma in glioma patients with Cav-1 overexpression/increased VM formation. Cav-1 promoted U251 glioma cell proliferation and VM formation in a Matrigel-based 3D culture model. VM-associated factors including hypoxia-inducible factor 1α (HIF-1α) and p-Akt was significantly elevated by Cav-1 overexpression but suppressed by siCav-1 in U251 cells. Collectively, our study identified Cav-1 as an important regulator of glioma cell proliferation and VM formation, contributing to glioma development and progression. Associação Brasileira de Divulgação Científica 2021-07-16 /pmc/articles/PMC8289350/ /pubmed/34287575 http://dx.doi.org/10.1590/1414-431X2020e10653 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Wenli
Cheng, Xing
Wang, Xiaobo
Hu, Wenjie
Wang, Jinshan
Liao, Chuangxin
Caveolin-1 promotes tumor cell proliferation and vasculogenic mimicry formation in human glioma
title Caveolin-1 promotes tumor cell proliferation and vasculogenic mimicry formation in human glioma
title_full Caveolin-1 promotes tumor cell proliferation and vasculogenic mimicry formation in human glioma
title_fullStr Caveolin-1 promotes tumor cell proliferation and vasculogenic mimicry formation in human glioma
title_full_unstemmed Caveolin-1 promotes tumor cell proliferation and vasculogenic mimicry formation in human glioma
title_short Caveolin-1 promotes tumor cell proliferation and vasculogenic mimicry formation in human glioma
title_sort caveolin-1 promotes tumor cell proliferation and vasculogenic mimicry formation in human glioma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289350/
https://www.ncbi.nlm.nih.gov/pubmed/34287575
http://dx.doi.org/10.1590/1414-431X2020e10653
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