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Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf‐5

Naphthenic acids (NAs) are carboxylic acids with the formula (C (n) H (2n) (+) (Z) O(2)) and are among the most toxic, persistent constituents of oil sands process‐affected waters (OSPW), produced during oil sands extraction. Currently, the proteins and mechanisms involved in NA biodegradation are u...

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Autores principales: McKew, Boyd A., Johnson, Richard, Clothier, Lindsay, Skeels, Karl, Ross, Matthew S., Metodiev, Metodi, Frenzel, Max, Gieg, Lisa M., Martin, Jonathan W., Hough, Michael A., Whitby, Corinne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289671/
https://www.ncbi.nlm.nih.gov/pubmed/34459546
http://dx.doi.org/10.1002/mbo3.1196
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author McKew, Boyd A.
Johnson, Richard
Clothier, Lindsay
Skeels, Karl
Ross, Matthew S.
Metodiev, Metodi
Frenzel, Max
Gieg, Lisa M.
Martin, Jonathan W.
Hough, Michael A.
Whitby, Corinne
author_facet McKew, Boyd A.
Johnson, Richard
Clothier, Lindsay
Skeels, Karl
Ross, Matthew S.
Metodiev, Metodi
Frenzel, Max
Gieg, Lisa M.
Martin, Jonathan W.
Hough, Michael A.
Whitby, Corinne
author_sort McKew, Boyd A.
collection PubMed
description Naphthenic acids (NAs) are carboxylic acids with the formula (C (n) H (2n) (+) (Z) O(2)) and are among the most toxic, persistent constituents of oil sands process‐affected waters (OSPW), produced during oil sands extraction. Currently, the proteins and mechanisms involved in NA biodegradation are unknown. Using LC‐MS/MS shotgun proteomics, we identified proteins overexpressed during the growth of Pseudomonas fluorescens Pf‐5 on a model NA (4′‐n‐butylphenyl)‐4‐butanoic acid (n‐BPBA) and commercial NA mixture (Acros). By day 11, >95% of n‐BPBA was degraded. With Acros, a 17% reduction in intensity occurred with 10–18 carbon compounds of the Z family −2 to −14 (major NA species in this mixture). A total of 554 proteins (n‐BPBA) and 631 proteins (Acros) were overexpressed during growth on NAs, including several transporters (e.g., ABC transporters), suggesting a cellular protective response from NA toxicity. Several proteins associated with fatty acid, lipid, and amino acid metabolism were also overexpressed, including acyl‐CoA dehydrogenase and acyl‐CoA thioesterase II, which catalyze part of the fatty acid beta‐oxidation pathway. Indeed, multiple enzymes involved in the fatty acid oxidation pathway were upregulated. Given the presumed structural similarity between alkyl‐carboxylic acid side chains and fatty acids, we postulate that P. fluorescens Pf‐5 was using existing fatty acid catabolic pathways (among others) during NA degradation.
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spelling pubmed-82896712021-07-21 Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf‐5 McKew, Boyd A. Johnson, Richard Clothier, Lindsay Skeels, Karl Ross, Matthew S. Metodiev, Metodi Frenzel, Max Gieg, Lisa M. Martin, Jonathan W. Hough, Michael A. Whitby, Corinne Microbiologyopen Original Articles Naphthenic acids (NAs) are carboxylic acids with the formula (C (n) H (2n) (+) (Z) O(2)) and are among the most toxic, persistent constituents of oil sands process‐affected waters (OSPW), produced during oil sands extraction. Currently, the proteins and mechanisms involved in NA biodegradation are unknown. Using LC‐MS/MS shotgun proteomics, we identified proteins overexpressed during the growth of Pseudomonas fluorescens Pf‐5 on a model NA (4′‐n‐butylphenyl)‐4‐butanoic acid (n‐BPBA) and commercial NA mixture (Acros). By day 11, >95% of n‐BPBA was degraded. With Acros, a 17% reduction in intensity occurred with 10–18 carbon compounds of the Z family −2 to −14 (major NA species in this mixture). A total of 554 proteins (n‐BPBA) and 631 proteins (Acros) were overexpressed during growth on NAs, including several transporters (e.g., ABC transporters), suggesting a cellular protective response from NA toxicity. Several proteins associated with fatty acid, lipid, and amino acid metabolism were also overexpressed, including acyl‐CoA dehydrogenase and acyl‐CoA thioesterase II, which catalyze part of the fatty acid beta‐oxidation pathway. Indeed, multiple enzymes involved in the fatty acid oxidation pathway were upregulated. Given the presumed structural similarity between alkyl‐carboxylic acid side chains and fatty acids, we postulate that P. fluorescens Pf‐5 was using existing fatty acid catabolic pathways (among others) during NA degradation. John Wiley and Sons Inc. 2021-07-19 /pmc/articles/PMC8289671/ /pubmed/34459546 http://dx.doi.org/10.1002/mbo3.1196 Text en © 2021 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
McKew, Boyd A.
Johnson, Richard
Clothier, Lindsay
Skeels, Karl
Ross, Matthew S.
Metodiev, Metodi
Frenzel, Max
Gieg, Lisa M.
Martin, Jonathan W.
Hough, Michael A.
Whitby, Corinne
Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf‐5
title Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf‐5
title_full Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf‐5
title_fullStr Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf‐5
title_full_unstemmed Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf‐5
title_short Differential protein expression during growth on model and commercial mixtures of naphthenic acids in Pseudomonas fluorescens Pf‐5
title_sort differential protein expression during growth on model and commercial mixtures of naphthenic acids in pseudomonas fluorescens pf‐5
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289671/
https://www.ncbi.nlm.nih.gov/pubmed/34459546
http://dx.doi.org/10.1002/mbo3.1196
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