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Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes

Listeria monocytogenes is a food-borne pathogen with the ability to grow at low temperatures down to − 0.4 °C. Maintaining cytoplasmic membrane fluidity by changing the lipid membrane composition is important during growth at low temperatures. In Listeria monocytogenes, the dominant adaptation effec...

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Detalles Bibliográficos
Autores principales: Flegler, Alexander, Kombeitz, Vanessa, Lipski, André
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289781/
https://www.ncbi.nlm.nih.gov/pubmed/33871675
http://dx.doi.org/10.1007/s00203-021-02322-6
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author Flegler, Alexander
Kombeitz, Vanessa
Lipski, André
author_facet Flegler, Alexander
Kombeitz, Vanessa
Lipski, André
author_sort Flegler, Alexander
collection PubMed
description Listeria monocytogenes is a food-borne pathogen with the ability to grow at low temperatures down to − 0.4 °C. Maintaining cytoplasmic membrane fluidity by changing the lipid membrane composition is important during growth at low temperatures. In Listeria monocytogenes, the dominant adaptation effect is the fluidization of the membrane by shortening of fatty acid chain length. In some strains, however, an additional response is the increase in menaquinone content during growth at low temperatures. The increase of this neutral lipid leads to fluidization of the membrane and thus represents a mechanism that is complementary to the fatty acid-mediated modification of membrane fluidity. This study demonstrated that the reduction of menaquinone content for Listeria monocytogenes strains resulted in significantly lower resistance to temperature stress and lower growth rates compared to unaffected control cultures after growth at 6 °C. Menaquinone content was reduced by supplementation with aromatic amino acids, which led to a feedback inhibition of the menaquinone synthesis. Menaquinone-reduced Listeria monocytogenes strains showed reduced bacterial cell fitness. This confirmed the adaptive function of menaquinones for growth at low temperatures of this pathogen.
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spelling pubmed-82897812021-08-05 Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes Flegler, Alexander Kombeitz, Vanessa Lipski, André Arch Microbiol Original Paper Listeria monocytogenes is a food-borne pathogen with the ability to grow at low temperatures down to − 0.4 °C. Maintaining cytoplasmic membrane fluidity by changing the lipid membrane composition is important during growth at low temperatures. In Listeria monocytogenes, the dominant adaptation effect is the fluidization of the membrane by shortening of fatty acid chain length. In some strains, however, an additional response is the increase in menaquinone content during growth at low temperatures. The increase of this neutral lipid leads to fluidization of the membrane and thus represents a mechanism that is complementary to the fatty acid-mediated modification of membrane fluidity. This study demonstrated that the reduction of menaquinone content for Listeria monocytogenes strains resulted in significantly lower resistance to temperature stress and lower growth rates compared to unaffected control cultures after growth at 6 °C. Menaquinone content was reduced by supplementation with aromatic amino acids, which led to a feedback inhibition of the menaquinone synthesis. Menaquinone-reduced Listeria monocytogenes strains showed reduced bacterial cell fitness. This confirmed the adaptive function of menaquinones for growth at low temperatures of this pathogen. Springer Berlin Heidelberg 2021-04-19 2021 /pmc/articles/PMC8289781/ /pubmed/33871675 http://dx.doi.org/10.1007/s00203-021-02322-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Flegler, Alexander
Kombeitz, Vanessa
Lipski, André
Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes
title Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes
title_full Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes
title_fullStr Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes
title_full_unstemmed Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes
title_short Menaquinone-mediated regulation of membrane fluidity is relevant for fitness of Listeria monocytogenes
title_sort menaquinone-mediated regulation of membrane fluidity is relevant for fitness of listeria monocytogenes
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8289781/
https://www.ncbi.nlm.nih.gov/pubmed/33871675
http://dx.doi.org/10.1007/s00203-021-02322-6
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