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The role of FOSL1 in stem-like cell reprogramming processes

Cancer stem-like cells (CSCs) have self-renewal abilities responsible for cancer progression, therapy resistance, and metastatic growth. The glioblastoma stem-like cells are the most studied among CSC populations. A recent study identified four transcription factors (SOX2, SALL2, OLIG2, and POU3F2)...

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Autores principales: Pecce, Valeria, Verrienti, Antonella, Fiscon, Giulia, Sponziello, Marialuisa, Conte, Federica, Abballe, Luana, Durante, Cosimo, Farina, Lorenzo, Filetti, Sebastiano, Paci, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290037/
https://www.ncbi.nlm.nih.gov/pubmed/34282187
http://dx.doi.org/10.1038/s41598-021-94072-0
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author Pecce, Valeria
Verrienti, Antonella
Fiscon, Giulia
Sponziello, Marialuisa
Conte, Federica
Abballe, Luana
Durante, Cosimo
Farina, Lorenzo
Filetti, Sebastiano
Paci, Paola
author_facet Pecce, Valeria
Verrienti, Antonella
Fiscon, Giulia
Sponziello, Marialuisa
Conte, Federica
Abballe, Luana
Durante, Cosimo
Farina, Lorenzo
Filetti, Sebastiano
Paci, Paola
author_sort Pecce, Valeria
collection PubMed
description Cancer stem-like cells (CSCs) have self-renewal abilities responsible for cancer progression, therapy resistance, and metastatic growth. The glioblastoma stem-like cells are the most studied among CSC populations. A recent study identified four transcription factors (SOX2, SALL2, OLIG2, and POU3F2) as the minimal core sufficient to reprogram differentiated glioblastoma (GBM) cells into stem-like cells. Transcriptomic data of GBM tissues and cell lines from two different datasets were then analyzed by the SWItch Miner (SWIM), a network-based software, and FOSL1 was identified as a putative regulator of the previously identified minimal core. Herein, we selected NTERA-2 and HEK293T cells to perform an in vitro study to investigate the role of FOSL1 in the reprogramming mechanisms. We transfected the two cell lines with a constitutive FOSL1 cDNA plasmid. We demonstrated that FOSL1 directly regulates the four transcription factors binding their promoter regions, is involved in the deregulation of several stemness markers, and reduces the cells’ ability to generate aggregates increasing the extracellular matrix component FN1. Although further experiments are necessary, our data suggest that FOSL1 reprograms the stemness by regulating the core of the four transcription factors.
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spelling pubmed-82900372021-07-21 The role of FOSL1 in stem-like cell reprogramming processes Pecce, Valeria Verrienti, Antonella Fiscon, Giulia Sponziello, Marialuisa Conte, Federica Abballe, Luana Durante, Cosimo Farina, Lorenzo Filetti, Sebastiano Paci, Paola Sci Rep Article Cancer stem-like cells (CSCs) have self-renewal abilities responsible for cancer progression, therapy resistance, and metastatic growth. The glioblastoma stem-like cells are the most studied among CSC populations. A recent study identified four transcription factors (SOX2, SALL2, OLIG2, and POU3F2) as the minimal core sufficient to reprogram differentiated glioblastoma (GBM) cells into stem-like cells. Transcriptomic data of GBM tissues and cell lines from two different datasets were then analyzed by the SWItch Miner (SWIM), a network-based software, and FOSL1 was identified as a putative regulator of the previously identified minimal core. Herein, we selected NTERA-2 and HEK293T cells to perform an in vitro study to investigate the role of FOSL1 in the reprogramming mechanisms. We transfected the two cell lines with a constitutive FOSL1 cDNA plasmid. We demonstrated that FOSL1 directly regulates the four transcription factors binding their promoter regions, is involved in the deregulation of several stemness markers, and reduces the cells’ ability to generate aggregates increasing the extracellular matrix component FN1. Although further experiments are necessary, our data suggest that FOSL1 reprograms the stemness by regulating the core of the four transcription factors. Nature Publishing Group UK 2021-07-19 /pmc/articles/PMC8290037/ /pubmed/34282187 http://dx.doi.org/10.1038/s41598-021-94072-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Pecce, Valeria
Verrienti, Antonella
Fiscon, Giulia
Sponziello, Marialuisa
Conte, Federica
Abballe, Luana
Durante, Cosimo
Farina, Lorenzo
Filetti, Sebastiano
Paci, Paola
The role of FOSL1 in stem-like cell reprogramming processes
title The role of FOSL1 in stem-like cell reprogramming processes
title_full The role of FOSL1 in stem-like cell reprogramming processes
title_fullStr The role of FOSL1 in stem-like cell reprogramming processes
title_full_unstemmed The role of FOSL1 in stem-like cell reprogramming processes
title_short The role of FOSL1 in stem-like cell reprogramming processes
title_sort role of fosl1 in stem-like cell reprogramming processes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290037/
https://www.ncbi.nlm.nih.gov/pubmed/34282187
http://dx.doi.org/10.1038/s41598-021-94072-0
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