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Jabuticaba [Plinia trunciflora (O. Berg) Kausel] Protects Liver of Diabetic Rats Against Mitochondrial Dysfunction and Oxidative Stress Through the Modulation of SIRT3 Expression

Complications generated by hyperglycemia present in diabetes mellitus (DM) have been constantly related to oxidative stress and dysfunction in the mitochondrial electron transport chain (ETC). Sirtuin 3 (SIRT3), which is present in mitochondria, is responsible for regulating several proteins involve...

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Autores principales: Calloni, Caroline, Martínez, Luana Soares, Gil, Daniela Franciele, da Silva, Douglas Machado, Jahn, Matheus Parmegiani, Salvador, Mirian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290295/
https://www.ncbi.nlm.nih.gov/pubmed/34295258
http://dx.doi.org/10.3389/fphys.2021.665747
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author Calloni, Caroline
Martínez, Luana Soares
Gil, Daniela Franciele
da Silva, Douglas Machado
Jahn, Matheus Parmegiani
Salvador, Mirian
author_facet Calloni, Caroline
Martínez, Luana Soares
Gil, Daniela Franciele
da Silva, Douglas Machado
Jahn, Matheus Parmegiani
Salvador, Mirian
author_sort Calloni, Caroline
collection PubMed
description Complications generated by hyperglycemia present in diabetes mellitus (DM) have been constantly related to oxidative stress and dysfunction in the mitochondrial electron transport chain (ETC). Sirtuin 3 (SIRT3), which is present in mitochondria, is responsible for regulating several proteins involved in metabolic homeostasis and oxidative stress. Studies have suggested alterations in the expression of SIRT3 in DM. The objective of this study was to evaluate the effects of phenolic compounds in jabuticaba (Plinia trunciflora), a berry native to Brazil, on the activity of mitochondrial ETC complexes, SIRT3 protein expression, and oxidative stress parameters in liver of diabetic rats induced by streptozotocin. After type 1 DM induction (streptozotocin 65 mg/kg), diabetic and healthy rats were treated with jabuticaba peel extract (JPE) by gavage (0.5 g/kg of weight) for 30 days. After treatments, those diabetic rats presented impaired activities of complexes I, II, and III of ETC along with an overexpression of SIRT3. In addition, an increase in lipid peroxidation and superoxide dismutase and catalase activities was observed in the diabetic group. The treatment with JPE was able to recover the activity of the mitochondrial complexes and reduce the expression of SIRT3. Furthermore, JPE treatment reduced oxidative damage to lipids and brought the antioxidants enzyme activities to basal levels in diabetic rats. Together, these results demonstrate that JPE can reduce oxidative stress related to DM by restoring mitochondrial complexes activity and regulating SIRT3 expression. Thus, JPE could become an alternative to reduce the development of complications related to DM.
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spelling pubmed-82902952021-07-21 Jabuticaba [Plinia trunciflora (O. Berg) Kausel] Protects Liver of Diabetic Rats Against Mitochondrial Dysfunction and Oxidative Stress Through the Modulation of SIRT3 Expression Calloni, Caroline Martínez, Luana Soares Gil, Daniela Franciele da Silva, Douglas Machado Jahn, Matheus Parmegiani Salvador, Mirian Front Physiol Physiology Complications generated by hyperglycemia present in diabetes mellitus (DM) have been constantly related to oxidative stress and dysfunction in the mitochondrial electron transport chain (ETC). Sirtuin 3 (SIRT3), which is present in mitochondria, is responsible for regulating several proteins involved in metabolic homeostasis and oxidative stress. Studies have suggested alterations in the expression of SIRT3 in DM. The objective of this study was to evaluate the effects of phenolic compounds in jabuticaba (Plinia trunciflora), a berry native to Brazil, on the activity of mitochondrial ETC complexes, SIRT3 protein expression, and oxidative stress parameters in liver of diabetic rats induced by streptozotocin. After type 1 DM induction (streptozotocin 65 mg/kg), diabetic and healthy rats were treated with jabuticaba peel extract (JPE) by gavage (0.5 g/kg of weight) for 30 days. After treatments, those diabetic rats presented impaired activities of complexes I, II, and III of ETC along with an overexpression of SIRT3. In addition, an increase in lipid peroxidation and superoxide dismutase and catalase activities was observed in the diabetic group. The treatment with JPE was able to recover the activity of the mitochondrial complexes and reduce the expression of SIRT3. Furthermore, JPE treatment reduced oxidative damage to lipids and brought the antioxidants enzyme activities to basal levels in diabetic rats. Together, these results demonstrate that JPE can reduce oxidative stress related to DM by restoring mitochondrial complexes activity and regulating SIRT3 expression. Thus, JPE could become an alternative to reduce the development of complications related to DM. Frontiers Media S.A. 2021-07-06 /pmc/articles/PMC8290295/ /pubmed/34295258 http://dx.doi.org/10.3389/fphys.2021.665747 Text en Copyright © 2021 Calloni, Martínez, Gil, da Silva, Jahn and Salvador. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Calloni, Caroline
Martínez, Luana Soares
Gil, Daniela Franciele
da Silva, Douglas Machado
Jahn, Matheus Parmegiani
Salvador, Mirian
Jabuticaba [Plinia trunciflora (O. Berg) Kausel] Protects Liver of Diabetic Rats Against Mitochondrial Dysfunction and Oxidative Stress Through the Modulation of SIRT3 Expression
title Jabuticaba [Plinia trunciflora (O. Berg) Kausel] Protects Liver of Diabetic Rats Against Mitochondrial Dysfunction and Oxidative Stress Through the Modulation of SIRT3 Expression
title_full Jabuticaba [Plinia trunciflora (O. Berg) Kausel] Protects Liver of Diabetic Rats Against Mitochondrial Dysfunction and Oxidative Stress Through the Modulation of SIRT3 Expression
title_fullStr Jabuticaba [Plinia trunciflora (O. Berg) Kausel] Protects Liver of Diabetic Rats Against Mitochondrial Dysfunction and Oxidative Stress Through the Modulation of SIRT3 Expression
title_full_unstemmed Jabuticaba [Plinia trunciflora (O. Berg) Kausel] Protects Liver of Diabetic Rats Against Mitochondrial Dysfunction and Oxidative Stress Through the Modulation of SIRT3 Expression
title_short Jabuticaba [Plinia trunciflora (O. Berg) Kausel] Protects Liver of Diabetic Rats Against Mitochondrial Dysfunction and Oxidative Stress Through the Modulation of SIRT3 Expression
title_sort jabuticaba [plinia trunciflora (o. berg) kausel] protects liver of diabetic rats against mitochondrial dysfunction and oxidative stress through the modulation of sirt3 expression
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290295/
https://www.ncbi.nlm.nih.gov/pubmed/34295258
http://dx.doi.org/10.3389/fphys.2021.665747
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