Cargando…

Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease

Intestinal tuberculosis (ITB) and Crohn’s disease (CD) are chronic inflammatory bowel disorders that are associated with dysregulated mucosal immunity. The gut microbiota plays an important role in the regulation of host immunity and inflammatory response. Although mounting evidence has linked CD wi...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Cong, Wang, Huan, Yu, Chen, Peng, Chao, Shu, Xu, Liao, Wangdi, Zhu, Zhenhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290844/
https://www.ncbi.nlm.nih.gov/pubmed/34295880
http://dx.doi.org/10.3389/fbioe.2021.673691
_version_ 1783724536451039232
author He, Cong
Wang, Huan
Yu, Chen
Peng, Chao
Shu, Xu
Liao, Wangdi
Zhu, Zhenhua
author_facet He, Cong
Wang, Huan
Yu, Chen
Peng, Chao
Shu, Xu
Liao, Wangdi
Zhu, Zhenhua
author_sort He, Cong
collection PubMed
description Intestinal tuberculosis (ITB) and Crohn’s disease (CD) are chronic inflammatory bowel disorders that are associated with dysregulated mucosal immunity. The gut microbiota plays an important role in the regulation of host immunity and inflammatory response. Although mounting evidence has linked CD with the dysbiosis of gut microbiota, the characteristic profiles of mucosal bacteria in ITB remain unclear. The aim of this study was to assess the alterations of the gut microbiota in ITB and compare the microbial structure of ITB with CD. A total of 71 mucosal samples were collected from patients with ITB, CD, and healthy controls (HC), and then, 16S rRNA gene sequencing was performed. The overall composition of gut microbiota in ITB was strikingly different from HC, with the dominance of Proteobacteria and reduction of Firmicutes. Of note, the short-chain fatty acids (SCFAs)-producing bacteria such as Faecalibacterium, Roseburia, and Ruminococcus were decreased in ITB relative to HC, while Klebsiella and Pseudomonas were enriched. Multiple predictive functional modules were altered in ITB, including the over-representation of lipopolysaccharide biosynthesis, bacterial invasion of epithelial cells, and pathogenic Escherichia coli infection that can promote inflammation. Additionally, the microbial structure in CD was distinctly different from ITB, characterized by lower alpha diversity and increased abundance of Bacteroides, Faecalibacterium, Collinsella, and Klebsiella. These four bacterial markers distinguished ITB from CD with an area under the curve of 97.6%. This study established the compositional and functional perturbation of the gut microbiome in ITB and suggested the potential for using gut microbiota as biomarkers to differentiate ITB from CD.
format Online
Article
Text
id pubmed-8290844
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82908442021-07-21 Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease He, Cong Wang, Huan Yu, Chen Peng, Chao Shu, Xu Liao, Wangdi Zhu, Zhenhua Front Bioeng Biotechnol Bioengineering and Biotechnology Intestinal tuberculosis (ITB) and Crohn’s disease (CD) are chronic inflammatory bowel disorders that are associated with dysregulated mucosal immunity. The gut microbiota plays an important role in the regulation of host immunity and inflammatory response. Although mounting evidence has linked CD with the dysbiosis of gut microbiota, the characteristic profiles of mucosal bacteria in ITB remain unclear. The aim of this study was to assess the alterations of the gut microbiota in ITB and compare the microbial structure of ITB with CD. A total of 71 mucosal samples were collected from patients with ITB, CD, and healthy controls (HC), and then, 16S rRNA gene sequencing was performed. The overall composition of gut microbiota in ITB was strikingly different from HC, with the dominance of Proteobacteria and reduction of Firmicutes. Of note, the short-chain fatty acids (SCFAs)-producing bacteria such as Faecalibacterium, Roseburia, and Ruminococcus were decreased in ITB relative to HC, while Klebsiella and Pseudomonas were enriched. Multiple predictive functional modules were altered in ITB, including the over-representation of lipopolysaccharide biosynthesis, bacterial invasion of epithelial cells, and pathogenic Escherichia coli infection that can promote inflammation. Additionally, the microbial structure in CD was distinctly different from ITB, characterized by lower alpha diversity and increased abundance of Bacteroides, Faecalibacterium, Collinsella, and Klebsiella. These four bacterial markers distinguished ITB from CD with an area under the curve of 97.6%. This study established the compositional and functional perturbation of the gut microbiome in ITB and suggested the potential for using gut microbiota as biomarkers to differentiate ITB from CD. Frontiers Media S.A. 2021-07-06 /pmc/articles/PMC8290844/ /pubmed/34295880 http://dx.doi.org/10.3389/fbioe.2021.673691 Text en Copyright © 2021 He, Wang, Yu, Peng, Shu, Liao and Zhu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
He, Cong
Wang, Huan
Yu, Chen
Peng, Chao
Shu, Xu
Liao, Wangdi
Zhu, Zhenhua
Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease
title Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease
title_full Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease
title_fullStr Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease
title_full_unstemmed Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease
title_short Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease
title_sort alterations of gut microbiota in patients with intestinal tuberculosis that different from crohn’s disease
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290844/
https://www.ncbi.nlm.nih.gov/pubmed/34295880
http://dx.doi.org/10.3389/fbioe.2021.673691
work_keys_str_mv AT hecong alterationsofgutmicrobiotainpatientswithintestinaltuberculosisthatdifferentfromcrohnsdisease
AT wanghuan alterationsofgutmicrobiotainpatientswithintestinaltuberculosisthatdifferentfromcrohnsdisease
AT yuchen alterationsofgutmicrobiotainpatientswithintestinaltuberculosisthatdifferentfromcrohnsdisease
AT pengchao alterationsofgutmicrobiotainpatientswithintestinaltuberculosisthatdifferentfromcrohnsdisease
AT shuxu alterationsofgutmicrobiotainpatientswithintestinaltuberculosisthatdifferentfromcrohnsdisease
AT liaowangdi alterationsofgutmicrobiotainpatientswithintestinaltuberculosisthatdifferentfromcrohnsdisease
AT zhuzhenhua alterationsofgutmicrobiotainpatientswithintestinaltuberculosisthatdifferentfromcrohnsdisease