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Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease
Intestinal tuberculosis (ITB) and Crohn’s disease (CD) are chronic inflammatory bowel disorders that are associated with dysregulated mucosal immunity. The gut microbiota plays an important role in the regulation of host immunity and inflammatory response. Although mounting evidence has linked CD wi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290844/ https://www.ncbi.nlm.nih.gov/pubmed/34295880 http://dx.doi.org/10.3389/fbioe.2021.673691 |
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author | He, Cong Wang, Huan Yu, Chen Peng, Chao Shu, Xu Liao, Wangdi Zhu, Zhenhua |
author_facet | He, Cong Wang, Huan Yu, Chen Peng, Chao Shu, Xu Liao, Wangdi Zhu, Zhenhua |
author_sort | He, Cong |
collection | PubMed |
description | Intestinal tuberculosis (ITB) and Crohn’s disease (CD) are chronic inflammatory bowel disorders that are associated with dysregulated mucosal immunity. The gut microbiota plays an important role in the regulation of host immunity and inflammatory response. Although mounting evidence has linked CD with the dysbiosis of gut microbiota, the characteristic profiles of mucosal bacteria in ITB remain unclear. The aim of this study was to assess the alterations of the gut microbiota in ITB and compare the microbial structure of ITB with CD. A total of 71 mucosal samples were collected from patients with ITB, CD, and healthy controls (HC), and then, 16S rRNA gene sequencing was performed. The overall composition of gut microbiota in ITB was strikingly different from HC, with the dominance of Proteobacteria and reduction of Firmicutes. Of note, the short-chain fatty acids (SCFAs)-producing bacteria such as Faecalibacterium, Roseburia, and Ruminococcus were decreased in ITB relative to HC, while Klebsiella and Pseudomonas were enriched. Multiple predictive functional modules were altered in ITB, including the over-representation of lipopolysaccharide biosynthesis, bacterial invasion of epithelial cells, and pathogenic Escherichia coli infection that can promote inflammation. Additionally, the microbial structure in CD was distinctly different from ITB, characterized by lower alpha diversity and increased abundance of Bacteroides, Faecalibacterium, Collinsella, and Klebsiella. These four bacterial markers distinguished ITB from CD with an area under the curve of 97.6%. This study established the compositional and functional perturbation of the gut microbiome in ITB and suggested the potential for using gut microbiota as biomarkers to differentiate ITB from CD. |
format | Online Article Text |
id | pubmed-8290844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82908442021-07-21 Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease He, Cong Wang, Huan Yu, Chen Peng, Chao Shu, Xu Liao, Wangdi Zhu, Zhenhua Front Bioeng Biotechnol Bioengineering and Biotechnology Intestinal tuberculosis (ITB) and Crohn’s disease (CD) are chronic inflammatory bowel disorders that are associated with dysregulated mucosal immunity. The gut microbiota plays an important role in the regulation of host immunity and inflammatory response. Although mounting evidence has linked CD with the dysbiosis of gut microbiota, the characteristic profiles of mucosal bacteria in ITB remain unclear. The aim of this study was to assess the alterations of the gut microbiota in ITB and compare the microbial structure of ITB with CD. A total of 71 mucosal samples were collected from patients with ITB, CD, and healthy controls (HC), and then, 16S rRNA gene sequencing was performed. The overall composition of gut microbiota in ITB was strikingly different from HC, with the dominance of Proteobacteria and reduction of Firmicutes. Of note, the short-chain fatty acids (SCFAs)-producing bacteria such as Faecalibacterium, Roseburia, and Ruminococcus were decreased in ITB relative to HC, while Klebsiella and Pseudomonas were enriched. Multiple predictive functional modules were altered in ITB, including the over-representation of lipopolysaccharide biosynthesis, bacterial invasion of epithelial cells, and pathogenic Escherichia coli infection that can promote inflammation. Additionally, the microbial structure in CD was distinctly different from ITB, characterized by lower alpha diversity and increased abundance of Bacteroides, Faecalibacterium, Collinsella, and Klebsiella. These four bacterial markers distinguished ITB from CD with an area under the curve of 97.6%. This study established the compositional and functional perturbation of the gut microbiome in ITB and suggested the potential for using gut microbiota as biomarkers to differentiate ITB from CD. Frontiers Media S.A. 2021-07-06 /pmc/articles/PMC8290844/ /pubmed/34295880 http://dx.doi.org/10.3389/fbioe.2021.673691 Text en Copyright © 2021 He, Wang, Yu, Peng, Shu, Liao and Zhu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology He, Cong Wang, Huan Yu, Chen Peng, Chao Shu, Xu Liao, Wangdi Zhu, Zhenhua Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease |
title | Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease |
title_full | Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease |
title_fullStr | Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease |
title_full_unstemmed | Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease |
title_short | Alterations of Gut Microbiota in Patients With Intestinal Tuberculosis That Different From Crohn’s Disease |
title_sort | alterations of gut microbiota in patients with intestinal tuberculosis that different from crohn’s disease |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290844/ https://www.ncbi.nlm.nih.gov/pubmed/34295880 http://dx.doi.org/10.3389/fbioe.2021.673691 |
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