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Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools

Clustered regularly interspaced short palindromic repeats (CRISPR) and accompanying CRISPR-associated (Cas) proteins provide RNA-guided adaptive immunity for prokaryotes to defend themselves against viruses. The CRISPR-Cas systems have attracted much attention in recent years for their power in aidi...

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Detalles Bibliográficos
Autores principales: Zhu, Yuwei, Huang, Zhiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291651/
https://www.ncbi.nlm.nih.gov/pubmed/34691893
http://dx.doi.org/10.1093/nsr/nwy150
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author Zhu, Yuwei
Huang, Zhiwei
author_facet Zhu, Yuwei
Huang, Zhiwei
author_sort Zhu, Yuwei
collection PubMed
description Clustered regularly interspaced short palindromic repeats (CRISPR) and accompanying CRISPR-associated (Cas) proteins provide RNA-guided adaptive immunity for prokaryotes to defend themselves against viruses. The CRISPR-Cas systems have attracted much attention in recent years for their power in aiding the development of genome editing tools. Based on the composition of the CRISPR RNA-effector complex, the CRISPR-Cas systems can be divided into two classes and six types. In this review, we summarize recent advances in the structural biology of the CRISPR-Cas-mediated genome editing tools, which helps us to understand the mechanism of how the guide RNAs assemble with diverse Cas proteins to cleave target nucleic acids.
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spelling pubmed-82916512021-10-21 Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools Zhu, Yuwei Huang, Zhiwei Natl Sci Rev BIOLOGY & BIOCHEMISTRY Clustered regularly interspaced short palindromic repeats (CRISPR) and accompanying CRISPR-associated (Cas) proteins provide RNA-guided adaptive immunity for prokaryotes to defend themselves against viruses. The CRISPR-Cas systems have attracted much attention in recent years for their power in aiding the development of genome editing tools. Based on the composition of the CRISPR RNA-effector complex, the CRISPR-Cas systems can be divided into two classes and six types. In this review, we summarize recent advances in the structural biology of the CRISPR-Cas-mediated genome editing tools, which helps us to understand the mechanism of how the guide RNAs assemble with diverse Cas proteins to cleave target nucleic acids. Oxford University Press 2019-05 2018-11-29 /pmc/articles/PMC8291651/ /pubmed/34691893 http://dx.doi.org/10.1093/nsr/nwy150 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle BIOLOGY & BIOCHEMISTRY
Zhu, Yuwei
Huang, Zhiwei
Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools
title Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools
title_full Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools
title_fullStr Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools
title_full_unstemmed Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools
title_short Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools
title_sort recent advances in structural studies of the crispr-cas-mediated genome editing tools
topic BIOLOGY & BIOCHEMISTRY
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291651/
https://www.ncbi.nlm.nih.gov/pubmed/34691893
http://dx.doi.org/10.1093/nsr/nwy150
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