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The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic
The coronavirus disease (COVID-19) is the global public health challenge currently persisting at a grand scale. A method that meets the rapid quantitative detection of antibodies to assess the body’s immune response from natural COVID-19 illness or vaccines’ effects is urgently needed. In the presen...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291656/ https://www.ncbi.nlm.nih.gov/pubmed/34283860 http://dx.doi.org/10.1371/journal.pone.0254486 |
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author | Chen, Pang-Yen Ko, Cheng-Hao Wang, C. Jason Chen, Chien-Wei Chiu, Wei-Huai Hong, Chitsung Cheng, Hao-Min Wang, I-Jen |
author_facet | Chen, Pang-Yen Ko, Cheng-Hao Wang, C. Jason Chen, Chien-Wei Chiu, Wei-Huai Hong, Chitsung Cheng, Hao-Min Wang, I-Jen |
author_sort | Chen, Pang-Yen |
collection | PubMed |
description | The coronavirus disease (COVID-19) is the global public health challenge currently persisting at a grand scale. A method that meets the rapid quantitative detection of antibodies to assess the body’s immune response from natural COVID-19 illness or vaccines’ effects is urgently needed. In the present study, an attempt was made to integrate a newly designed spectrometer to the COVID-19 test strip procedure; this augmentation provides the quantitative capacity to a lateral flow immunoassay (LFIA). Optical interpretation of results by quantitative α index, rather than visual qualification, can be done quickly, in 5–10 minutes. The developed product was compared with several other serological IgM/IgG antibody reagents on the market by recruiting 111 participants suspected of having COVID-19 infection from March to May 2020 in a hospital. Taking RT-PCR as the diagnostic gold standard, the quantitative spectral LIFA platform could correctly detect all 12 COVID-19 patients. Concerning RT-PCR negative patients, all three antibody testing methods found positive cases. The optical-based platform exhibited the ability of early detection of immunoglobulins of RT-PCR negative patients. There was an apparent trend that elevation of IgM levels in the acute phase of infection; then IgG levels rose later. It exhibited the risk of a false-negative diagnosis of RT-PCR in COVID-19 testing. The significant detection ability of this new optical-based platform demonstrated clinical potential. |
format | Online Article Text |
id | pubmed-8291656 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-82916562021-07-31 The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic Chen, Pang-Yen Ko, Cheng-Hao Wang, C. Jason Chen, Chien-Wei Chiu, Wei-Huai Hong, Chitsung Cheng, Hao-Min Wang, I-Jen PLoS One Research Article The coronavirus disease (COVID-19) is the global public health challenge currently persisting at a grand scale. A method that meets the rapid quantitative detection of antibodies to assess the body’s immune response from natural COVID-19 illness or vaccines’ effects is urgently needed. In the present study, an attempt was made to integrate a newly designed spectrometer to the COVID-19 test strip procedure; this augmentation provides the quantitative capacity to a lateral flow immunoassay (LFIA). Optical interpretation of results by quantitative α index, rather than visual qualification, can be done quickly, in 5–10 minutes. The developed product was compared with several other serological IgM/IgG antibody reagents on the market by recruiting 111 participants suspected of having COVID-19 infection from March to May 2020 in a hospital. Taking RT-PCR as the diagnostic gold standard, the quantitative spectral LIFA platform could correctly detect all 12 COVID-19 patients. Concerning RT-PCR negative patients, all three antibody testing methods found positive cases. The optical-based platform exhibited the ability of early detection of immunoglobulins of RT-PCR negative patients. There was an apparent trend that elevation of IgM levels in the acute phase of infection; then IgG levels rose later. It exhibited the risk of a false-negative diagnosis of RT-PCR in COVID-19 testing. The significant detection ability of this new optical-based platform demonstrated clinical potential. Public Library of Science 2021-07-20 /pmc/articles/PMC8291656/ /pubmed/34283860 http://dx.doi.org/10.1371/journal.pone.0254486 Text en © 2021 Chen et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Chen, Pang-Yen Ko, Cheng-Hao Wang, C. Jason Chen, Chien-Wei Chiu, Wei-Huai Hong, Chitsung Cheng, Hao-Min Wang, I-Jen The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic |
title | The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic |
title_full | The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic |
title_fullStr | The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic |
title_full_unstemmed | The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic |
title_short | The early detection of immunoglobulins via optical-based lateral flow immunoassay platform in COVID-19 pandemic |
title_sort | early detection of immunoglobulins via optical-based lateral flow immunoassay platform in covid-19 pandemic |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291656/ https://www.ncbi.nlm.nih.gov/pubmed/34283860 http://dx.doi.org/10.1371/journal.pone.0254486 |
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