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A species-specific functional module controls formation of pollen apertures

Pollen apertures are an interesting model for the formation of specialized plasma-membrane domains. The plant-specific protein INP1 serves as a key aperture factor in such distantly related species as Arabidopsis, rice, and maize. Although INP1 orthologs likely play similar roles throughout flowerin...

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Detalles Bibliográficos
Autores principales: Lee, Byung Ha, Wang, Rui, Moberg, Ingrid M., Reeder, Sarah H., Amom, Prativa, Tan, Michelle H., Amstutz, Katelyn, Chandna, Pallavi, Helton, Adam, Andrianova, Ekaterina P., Zhulin, Igor B., Dobritsa, Anna A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292223/
https://www.ncbi.nlm.nih.gov/pubmed/34183783
http://dx.doi.org/10.1038/s41477-021-00951-9
Descripción
Sumario:Pollen apertures are an interesting model for the formation of specialized plasma-membrane domains. The plant-specific protein INP1 serves as a key aperture factor in such distantly related species as Arabidopsis, rice, and maize. Although INP1 orthologs likely play similar roles throughout flowering plants, they show significant sequence divergence and often cannot substitute for each other, suggesting that INP1 might require species-specific partners. Here, we present a new aperture factor, INP2, which satisfies the criteria for being a species-specific partner for INP1. Both INP proteins display similar structural features, including the plant-specific DOG1 domain, similar patterns of expression and mutant phenotypes, as well as signs of co-evolution. These proteins interact with each other in a species-specific manner and can restore apertures in a heterologous system when both are expressed, but not when expressed individually. Our findings suggest that the INP proteins form a species-specific functional module that underlies formation of pollen apertures.