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A species-specific functional module controls formation of pollen apertures
Pollen apertures are an interesting model for the formation of specialized plasma-membrane domains. The plant-specific protein INP1 serves as a key aperture factor in such distantly related species as Arabidopsis, rice, and maize. Although INP1 orthologs likely play similar roles throughout flowerin...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292223/ https://www.ncbi.nlm.nih.gov/pubmed/34183783 http://dx.doi.org/10.1038/s41477-021-00951-9 |
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author | Lee, Byung Ha Wang, Rui Moberg, Ingrid M. Reeder, Sarah H. Amom, Prativa Tan, Michelle H. Amstutz, Katelyn Chandna, Pallavi Helton, Adam Andrianova, Ekaterina P. Zhulin, Igor B. Dobritsa, Anna A. |
author_facet | Lee, Byung Ha Wang, Rui Moberg, Ingrid M. Reeder, Sarah H. Amom, Prativa Tan, Michelle H. Amstutz, Katelyn Chandna, Pallavi Helton, Adam Andrianova, Ekaterina P. Zhulin, Igor B. Dobritsa, Anna A. |
author_sort | Lee, Byung Ha |
collection | PubMed |
description | Pollen apertures are an interesting model for the formation of specialized plasma-membrane domains. The plant-specific protein INP1 serves as a key aperture factor in such distantly related species as Arabidopsis, rice, and maize. Although INP1 orthologs likely play similar roles throughout flowering plants, they show significant sequence divergence and often cannot substitute for each other, suggesting that INP1 might require species-specific partners. Here, we present a new aperture factor, INP2, which satisfies the criteria for being a species-specific partner for INP1. Both INP proteins display similar structural features, including the plant-specific DOG1 domain, similar patterns of expression and mutant phenotypes, as well as signs of co-evolution. These proteins interact with each other in a species-specific manner and can restore apertures in a heterologous system when both are expressed, but not when expressed individually. Our findings suggest that the INP proteins form a species-specific functional module that underlies formation of pollen apertures. |
format | Online Article Text |
id | pubmed-8292223 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
record_format | MEDLINE/PubMed |
spelling | pubmed-82922232021-12-28 A species-specific functional module controls formation of pollen apertures Lee, Byung Ha Wang, Rui Moberg, Ingrid M. Reeder, Sarah H. Amom, Prativa Tan, Michelle H. Amstutz, Katelyn Chandna, Pallavi Helton, Adam Andrianova, Ekaterina P. Zhulin, Igor B. Dobritsa, Anna A. Nat Plants Article Pollen apertures are an interesting model for the formation of specialized plasma-membrane domains. The plant-specific protein INP1 serves as a key aperture factor in such distantly related species as Arabidopsis, rice, and maize. Although INP1 orthologs likely play similar roles throughout flowering plants, they show significant sequence divergence and often cannot substitute for each other, suggesting that INP1 might require species-specific partners. Here, we present a new aperture factor, INP2, which satisfies the criteria for being a species-specific partner for INP1. Both INP proteins display similar structural features, including the plant-specific DOG1 domain, similar patterns of expression and mutant phenotypes, as well as signs of co-evolution. These proteins interact with each other in a species-specific manner and can restore apertures in a heterologous system when both are expressed, but not when expressed individually. Our findings suggest that the INP proteins form a species-specific functional module that underlies formation of pollen apertures. 2021-06-28 2021-07 /pmc/articles/PMC8292223/ /pubmed/34183783 http://dx.doi.org/10.1038/s41477-021-00951-9 Text en http://www.nature.com/authors/editorial_policies/license.html#termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Lee, Byung Ha Wang, Rui Moberg, Ingrid M. Reeder, Sarah H. Amom, Prativa Tan, Michelle H. Amstutz, Katelyn Chandna, Pallavi Helton, Adam Andrianova, Ekaterina P. Zhulin, Igor B. Dobritsa, Anna A. A species-specific functional module controls formation of pollen apertures |
title | A species-specific functional module controls formation of pollen apertures |
title_full | A species-specific functional module controls formation of pollen apertures |
title_fullStr | A species-specific functional module controls formation of pollen apertures |
title_full_unstemmed | A species-specific functional module controls formation of pollen apertures |
title_short | A species-specific functional module controls formation of pollen apertures |
title_sort | species-specific functional module controls formation of pollen apertures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292223/ https://www.ncbi.nlm.nih.gov/pubmed/34183783 http://dx.doi.org/10.1038/s41477-021-00951-9 |
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