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Proteomic Characterization of Canine Gastric Fluid by Liquid Chromatography–Mass Spectrometry for Development of Protein Biomarkers in Regurgitation, Vomiting, and Cough

Reflux and aspiration in people cause and exacerbate respiratory diseases in the absence of gastrointestinal signs. Protein biomarkers in humans detect extraesophageal reflux (EER) from oropharyngeal (OP) and bronchoalveloar lavage samples. Reflux likely contributes to respiratory disease in dogs. T...

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Autores principales: Grobman, Megan, Rindt, Hansjörg, Reinero, Carol R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292676/
https://www.ncbi.nlm.nih.gov/pubmed/34307522
http://dx.doi.org/10.3389/fvets.2021.670007
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author Grobman, Megan
Rindt, Hansjörg
Reinero, Carol R.
author_facet Grobman, Megan
Rindt, Hansjörg
Reinero, Carol R.
author_sort Grobman, Megan
collection PubMed
description Reflux and aspiration in people cause and exacerbate respiratory diseases in the absence of gastrointestinal signs. Protein biomarkers in humans detect extraesophageal reflux (EER) from oropharyngeal (OP) and bronchoalveloar lavage samples. Reflux likely contributes to respiratory disease in dogs. The objectives of this study were to analyze the canine gastric fluid (GF) proteome and compare this to the OP proteome in normal, vomiting/regurgitating, and coughing dogs to identify biomarkers for EER/aspiration. Twenty-three client-owned dogs were enrolled. Canine GF samples (n = 5) and OP swabs in normal (n = 6), vomiting/regurgitating (n = 7), and coughing (n = 5) dogs were within 2 weeks of sample collection. Protein digests were analyzed by liquid chromatography–mass spectrometry. Differential abundance (DA) of proteins between groups was evaluated by Fisher's exact test with p < 0.0004 significance level after correction for multiple comparisons. DA was found between all groups (p < 0.0001): GF vs. normal (n = 130 proteins), coughing vs. normal (n = 22 proteins), and vomiting/regurgitating vs. normal (n = 20 proteins). Protein abundance was highly variable between dogs. Gastrointestinal-specific proteins were found in OP swabs from vomiting/regurgitating and coughing dogs but not from healthy dogs. In conclusion, the proteomic composition of the OP varies between health and disease. The presence of gastrointestinal-specific proteins in OP of coughing dogs may suggest reflux and/or aspiration as contributing factors. The variable protein abundance warrants investigation into biomarker panels.
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spelling pubmed-82926762021-07-22 Proteomic Characterization of Canine Gastric Fluid by Liquid Chromatography–Mass Spectrometry for Development of Protein Biomarkers in Regurgitation, Vomiting, and Cough Grobman, Megan Rindt, Hansjörg Reinero, Carol R. Front Vet Sci Veterinary Science Reflux and aspiration in people cause and exacerbate respiratory diseases in the absence of gastrointestinal signs. Protein biomarkers in humans detect extraesophageal reflux (EER) from oropharyngeal (OP) and bronchoalveloar lavage samples. Reflux likely contributes to respiratory disease in dogs. The objectives of this study were to analyze the canine gastric fluid (GF) proteome and compare this to the OP proteome in normal, vomiting/regurgitating, and coughing dogs to identify biomarkers for EER/aspiration. Twenty-three client-owned dogs were enrolled. Canine GF samples (n = 5) and OP swabs in normal (n = 6), vomiting/regurgitating (n = 7), and coughing (n = 5) dogs were within 2 weeks of sample collection. Protein digests were analyzed by liquid chromatography–mass spectrometry. Differential abundance (DA) of proteins between groups was evaluated by Fisher's exact test with p < 0.0004 significance level after correction for multiple comparisons. DA was found between all groups (p < 0.0001): GF vs. normal (n = 130 proteins), coughing vs. normal (n = 22 proteins), and vomiting/regurgitating vs. normal (n = 20 proteins). Protein abundance was highly variable between dogs. Gastrointestinal-specific proteins were found in OP swabs from vomiting/regurgitating and coughing dogs but not from healthy dogs. In conclusion, the proteomic composition of the OP varies between health and disease. The presence of gastrointestinal-specific proteins in OP of coughing dogs may suggest reflux and/or aspiration as contributing factors. The variable protein abundance warrants investigation into biomarker panels. Frontiers Media S.A. 2021-07-07 /pmc/articles/PMC8292676/ /pubmed/34307522 http://dx.doi.org/10.3389/fvets.2021.670007 Text en Copyright © 2021 Grobman, Rindt and Reinero. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Grobman, Megan
Rindt, Hansjörg
Reinero, Carol R.
Proteomic Characterization of Canine Gastric Fluid by Liquid Chromatography–Mass Spectrometry for Development of Protein Biomarkers in Regurgitation, Vomiting, and Cough
title Proteomic Characterization of Canine Gastric Fluid by Liquid Chromatography–Mass Spectrometry for Development of Protein Biomarkers in Regurgitation, Vomiting, and Cough
title_full Proteomic Characterization of Canine Gastric Fluid by Liquid Chromatography–Mass Spectrometry for Development of Protein Biomarkers in Regurgitation, Vomiting, and Cough
title_fullStr Proteomic Characterization of Canine Gastric Fluid by Liquid Chromatography–Mass Spectrometry for Development of Protein Biomarkers in Regurgitation, Vomiting, and Cough
title_full_unstemmed Proteomic Characterization of Canine Gastric Fluid by Liquid Chromatography–Mass Spectrometry for Development of Protein Biomarkers in Regurgitation, Vomiting, and Cough
title_short Proteomic Characterization of Canine Gastric Fluid by Liquid Chromatography–Mass Spectrometry for Development of Protein Biomarkers in Regurgitation, Vomiting, and Cough
title_sort proteomic characterization of canine gastric fluid by liquid chromatography–mass spectrometry for development of protein biomarkers in regurgitation, vomiting, and cough
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292676/
https://www.ncbi.nlm.nih.gov/pubmed/34307522
http://dx.doi.org/10.3389/fvets.2021.670007
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