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C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma

Glycosyltransferases are frequently dysregulated in lung cancer. Core 1 β 1, 3-galactosyltransferase 1 (C1GALT1), an enzyme highly expressed in various cancers, is correlated with tumor initiation and development. However, the role of C1GALT1 in lung cancer remains poorly understood. In this study,...

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Autores principales: Dong, Xiaoxia, Liu, Yongyu, Deng, Xinzhou, Shao, Jun, Tian, Shuangyue, Chen, Shuang, Huang, Rongxin, Lin, Ziao, Chen, Chunli, Shen, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292976/
https://www.ncbi.nlm.nih.gov/pubmed/34307388
http://dx.doi.org/10.3389/fcell.2021.707970
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author Dong, Xiaoxia
Liu, Yongyu
Deng, Xinzhou
Shao, Jun
Tian, Shuangyue
Chen, Shuang
Huang, Rongxin
Lin, Ziao
Chen, Chunli
Shen, Li
author_facet Dong, Xiaoxia
Liu, Yongyu
Deng, Xinzhou
Shao, Jun
Tian, Shuangyue
Chen, Shuang
Huang, Rongxin
Lin, Ziao
Chen, Chunli
Shen, Li
author_sort Dong, Xiaoxia
collection PubMed
description Glycosyltransferases are frequently dysregulated in lung cancer. Core 1 β 1, 3-galactosyltransferase 1 (C1GALT1), an enzyme highly expressed in various cancers, is correlated with tumor initiation and development. However, the role of C1GALT1 in lung cancer remains poorly understood. In this study, through bioinformatic analysis and clinical validation, we first discovered that C1GALT1 expression was upregulated in lung adenocarcinoma (LUAD) tissues and was closely related to poor prognosis in patients with LUAD. Gain- and loss-of-function experiments showed that C1GALT1 promoted LUAD cell proliferation, migration, and invasion in vitro, as well as tumor formation in vivo. Further investigation demonstrated that RAC1 expression was positively regulated by C1GALT1 in LUAD, whereas silencing Rac1 could reverse C1GALT1-induced tumor growth and metastasis. Moreover, miR-181d-5p was identified as a negative regulator for C1GALT1 in LUAD. As expected, the inhibitory effects of miR-181d-5p on LUAD cell proliferation, migration, and invasion were counteracted by restoration of C1GALT1. In summary, our results highlight the importance of the miR-181d-5p/C1GALT1/RAC1 regulatory axis during LUAD progression. Thus, C1GALT1 may serve as a potential therapeutic target for LUAD.
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spelling pubmed-82929762021-07-22 C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma Dong, Xiaoxia Liu, Yongyu Deng, Xinzhou Shao, Jun Tian, Shuangyue Chen, Shuang Huang, Rongxin Lin, Ziao Chen, Chunli Shen, Li Front Cell Dev Biol Cell and Developmental Biology Glycosyltransferases are frequently dysregulated in lung cancer. Core 1 β 1, 3-galactosyltransferase 1 (C1GALT1), an enzyme highly expressed in various cancers, is correlated with tumor initiation and development. However, the role of C1GALT1 in lung cancer remains poorly understood. In this study, through bioinformatic analysis and clinical validation, we first discovered that C1GALT1 expression was upregulated in lung adenocarcinoma (LUAD) tissues and was closely related to poor prognosis in patients with LUAD. Gain- and loss-of-function experiments showed that C1GALT1 promoted LUAD cell proliferation, migration, and invasion in vitro, as well as tumor formation in vivo. Further investigation demonstrated that RAC1 expression was positively regulated by C1GALT1 in LUAD, whereas silencing Rac1 could reverse C1GALT1-induced tumor growth and metastasis. Moreover, miR-181d-5p was identified as a negative regulator for C1GALT1 in LUAD. As expected, the inhibitory effects of miR-181d-5p on LUAD cell proliferation, migration, and invasion were counteracted by restoration of C1GALT1. In summary, our results highlight the importance of the miR-181d-5p/C1GALT1/RAC1 regulatory axis during LUAD progression. Thus, C1GALT1 may serve as a potential therapeutic target for LUAD. Frontiers Media S.A. 2021-07-07 /pmc/articles/PMC8292976/ /pubmed/34307388 http://dx.doi.org/10.3389/fcell.2021.707970 Text en Copyright © 2021 Dong, Liu, Deng, Shao, Tian, Chen, Huang, Lin, Chen and Shen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Dong, Xiaoxia
Liu, Yongyu
Deng, Xinzhou
Shao, Jun
Tian, Shuangyue
Chen, Shuang
Huang, Rongxin
Lin, Ziao
Chen, Chunli
Shen, Li
C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma
title C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma
title_full C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma
title_fullStr C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma
title_full_unstemmed C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma
title_short C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma
title_sort c1galt1, negatively regulated by mir-181d-5p, promotes tumor progression via upregulating rac1 in lung adenocarcinoma
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292976/
https://www.ncbi.nlm.nih.gov/pubmed/34307388
http://dx.doi.org/10.3389/fcell.2021.707970
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