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The Expression of Transcription Factors in Fetal Lamb Kidney

(1) Background: Renal development involves frequent expression and loss of transcription factors, resulting in the activation of genes. Wilms’ tumor 1 (WT1), hepatocyte nuclear factor-1-beta (HNF1β), and paired box genes 2 and 8 (Pax2 and Pax8) play an important role in renal development. With this...

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Autores principales: Nishiya, Yuri, Kawaguchi, Kohei, Kudo, Kosuke, Kawaguchi, Takuya, Obayashi, Juma, Tanaka, Kunihide, Ohyama, Kei, Nagae, Hideki, Furuta, Shigeyuki, Seki, Yasuji, Koike, Junki, Pringle, Kevin C., Kitagawa, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293116/
https://www.ncbi.nlm.nih.gov/pubmed/34205452
http://dx.doi.org/10.3390/jdb9020022
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author Nishiya, Yuri
Kawaguchi, Kohei
Kudo, Kosuke
Kawaguchi, Takuya
Obayashi, Juma
Tanaka, Kunihide
Ohyama, Kei
Nagae, Hideki
Furuta, Shigeyuki
Seki, Yasuji
Koike, Junki
Pringle, Kevin C.
Kitagawa, Hiroaki
author_facet Nishiya, Yuri
Kawaguchi, Kohei
Kudo, Kosuke
Kawaguchi, Takuya
Obayashi, Juma
Tanaka, Kunihide
Ohyama, Kei
Nagae, Hideki
Furuta, Shigeyuki
Seki, Yasuji
Koike, Junki
Pringle, Kevin C.
Kitagawa, Hiroaki
author_sort Nishiya, Yuri
collection PubMed
description (1) Background: Renal development involves frequent expression and loss of transcription factors, resulting in the activation of genes. Wilms’ tumor 1 (WT1), hepatocyte nuclear factor-1-beta (HNF1β), and paired box genes 2 and 8 (Pax2 and Pax8) play an important role in renal development. With this in vivo study, we examined the period and location of expression of these factors in renal development. (2) Methods: Fetal lamb kidneys (50 days from gestation to term) and adult ewe kidneys were evaluated by hematoxylin and eosin staining. Serial sections were subjected to immunohistochemistry for WT1, HNF1β, Pax2, and Pax8. (3) Results: Pax2, Pax8, and HNF1β expression was observed in the ureteric bud and collecting duct epithelial cells. We observed expression of WT1 alone in metanephric mesenchymal cells, glomerular epithelial cells, and interstitial cells in the medullary rays and Pax8 and HNF1β expression in tubular epithelial cells. WT1 was highly expressed in cells more proximal to the medulla in renal vesicles and in C- and S-shaped bodies. Pax2 was expressed in the middle and peripheral regions, and HNF1β in cells in the region in the middle of these. (4) Conclusions: WT1 is involved in nephron development. Pax2, Pax8, and HNF1β are involved in nephron maturation and the formation of peripheral collecting ducts from the Wolffian duct.
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spelling pubmed-82931162021-07-22 The Expression of Transcription Factors in Fetal Lamb Kidney Nishiya, Yuri Kawaguchi, Kohei Kudo, Kosuke Kawaguchi, Takuya Obayashi, Juma Tanaka, Kunihide Ohyama, Kei Nagae, Hideki Furuta, Shigeyuki Seki, Yasuji Koike, Junki Pringle, Kevin C. Kitagawa, Hiroaki J Dev Biol Article (1) Background: Renal development involves frequent expression and loss of transcription factors, resulting in the activation of genes. Wilms’ tumor 1 (WT1), hepatocyte nuclear factor-1-beta (HNF1β), and paired box genes 2 and 8 (Pax2 and Pax8) play an important role in renal development. With this in vivo study, we examined the period and location of expression of these factors in renal development. (2) Methods: Fetal lamb kidneys (50 days from gestation to term) and adult ewe kidneys were evaluated by hematoxylin and eosin staining. Serial sections were subjected to immunohistochemistry for WT1, HNF1β, Pax2, and Pax8. (3) Results: Pax2, Pax8, and HNF1β expression was observed in the ureteric bud and collecting duct epithelial cells. We observed expression of WT1 alone in metanephric mesenchymal cells, glomerular epithelial cells, and interstitial cells in the medullary rays and Pax8 and HNF1β expression in tubular epithelial cells. WT1 was highly expressed in cells more proximal to the medulla in renal vesicles and in C- and S-shaped bodies. Pax2 was expressed in the middle and peripheral regions, and HNF1β in cells in the region in the middle of these. (4) Conclusions: WT1 is involved in nephron development. Pax2, Pax8, and HNF1β are involved in nephron maturation and the formation of peripheral collecting ducts from the Wolffian duct. MDPI 2021-06-19 /pmc/articles/PMC8293116/ /pubmed/34205452 http://dx.doi.org/10.3390/jdb9020022 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nishiya, Yuri
Kawaguchi, Kohei
Kudo, Kosuke
Kawaguchi, Takuya
Obayashi, Juma
Tanaka, Kunihide
Ohyama, Kei
Nagae, Hideki
Furuta, Shigeyuki
Seki, Yasuji
Koike, Junki
Pringle, Kevin C.
Kitagawa, Hiroaki
The Expression of Transcription Factors in Fetal Lamb Kidney
title The Expression of Transcription Factors in Fetal Lamb Kidney
title_full The Expression of Transcription Factors in Fetal Lamb Kidney
title_fullStr The Expression of Transcription Factors in Fetal Lamb Kidney
title_full_unstemmed The Expression of Transcription Factors in Fetal Lamb Kidney
title_short The Expression of Transcription Factors in Fetal Lamb Kidney
title_sort expression of transcription factors in fetal lamb kidney
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293116/
https://www.ncbi.nlm.nih.gov/pubmed/34205452
http://dx.doi.org/10.3390/jdb9020022
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