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Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of p...

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Autores principales: Nath, Peuli, Kabir, Md Alamgir, Doust, Somaiyeh Khoubafarin, Ray, Aniruddha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293188/
https://www.ncbi.nlm.nih.gov/pubmed/34199547
http://dx.doi.org/10.3390/idr13020049
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author Nath, Peuli
Kabir, Md Alamgir
Doust, Somaiyeh Khoubafarin
Ray, Aniruddha
author_facet Nath, Peuli
Kabir, Md Alamgir
Doust, Somaiyeh Khoubafarin
Ray, Aniruddha
author_sort Nath, Peuli
collection PubMed
description Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages.
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spelling pubmed-82931882021-07-22 Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques Nath, Peuli Kabir, Md Alamgir Doust, Somaiyeh Khoubafarin Ray, Aniruddha Infect Dis Rep Review Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages. MDPI 2021-06-02 /pmc/articles/PMC8293188/ /pubmed/34199547 http://dx.doi.org/10.3390/idr13020049 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Nath, Peuli
Kabir, Md Alamgir
Doust, Somaiyeh Khoubafarin
Ray, Aniruddha
Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques
title Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques
title_full Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques
title_fullStr Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques
title_full_unstemmed Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques
title_short Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques
title_sort diagnosis of herpes simplex virus: laboratory and point-of-care techniques
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293188/
https://www.ncbi.nlm.nih.gov/pubmed/34199547
http://dx.doi.org/10.3390/idr13020049
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