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Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques
Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of p...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293188/ https://www.ncbi.nlm.nih.gov/pubmed/34199547 http://dx.doi.org/10.3390/idr13020049 |
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author | Nath, Peuli Kabir, Md Alamgir Doust, Somaiyeh Khoubafarin Ray, Aniruddha |
author_facet | Nath, Peuli Kabir, Md Alamgir Doust, Somaiyeh Khoubafarin Ray, Aniruddha |
author_sort | Nath, Peuli |
collection | PubMed |
description | Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages. |
format | Online Article Text |
id | pubmed-8293188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82931882021-07-22 Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques Nath, Peuli Kabir, Md Alamgir Doust, Somaiyeh Khoubafarin Ray, Aniruddha Infect Dis Rep Review Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages. MDPI 2021-06-02 /pmc/articles/PMC8293188/ /pubmed/34199547 http://dx.doi.org/10.3390/idr13020049 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Nath, Peuli Kabir, Md Alamgir Doust, Somaiyeh Khoubafarin Ray, Aniruddha Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques |
title | Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques |
title_full | Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques |
title_fullStr | Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques |
title_full_unstemmed | Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques |
title_short | Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques |
title_sort | diagnosis of herpes simplex virus: laboratory and point-of-care techniques |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293188/ https://www.ncbi.nlm.nih.gov/pubmed/34199547 http://dx.doi.org/10.3390/idr13020049 |
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