Cargando…
5-Hydroxymethylcytosine Loss in Conjunctival Melanoma
Aims: Conjunctival and cutaneous melanoma partially share similar clinical and molecular backgrounds. As 5-hydroxymethylcytosine (5-hmC) loss has been demonstrated in cutaneous melanoma, we decided to assess if similar changes were occurring in conjunctival melanoma. Methods: 5-methylcytosine (5-mC)...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293194/ https://www.ncbi.nlm.nih.gov/pubmed/34198758 http://dx.doi.org/10.3390/dermatopathology8020023 |
_version_ | 1783724980952891392 |
---|---|
author | Stahl, Alexandre Riggi, Nicolo Nardou, Katya Nicolas, Michael Kaya, Gurkan Moulin, Alexandre |
author_facet | Stahl, Alexandre Riggi, Nicolo Nardou, Katya Nicolas, Michael Kaya, Gurkan Moulin, Alexandre |
author_sort | Stahl, Alexandre |
collection | PubMed |
description | Aims: Conjunctival and cutaneous melanoma partially share similar clinical and molecular backgrounds. As 5-hydroxymethylcytosine (5-hmC) loss has been demonstrated in cutaneous melanoma, we decided to assess if similar changes were occurring in conjunctival melanoma. Methods: 5-methylcytosine (5-mC), 5-hmC and TET2 were respectively identified by immunohistochemistry and RNA ISH in 40 conjunctival nevi and 37 conjunctival melanomas. Clinicopathological correlations were established. Results: 5-mC, TET2 and 5-hmC were respectively identified in 67.5%, 95% and 100% of conjunctival nevi and in 81.1%, 35.1% and 54% of conjunctival melanomas. A significant 5-hmC and TET2 loss was identified in conjunctival melanoma comparing to nevus, as well as a significant correlation between TET2 and 5-hmC expression. In the melanomas, 5-hmC expression was only significantly associated with local lymphatic invasion, but not with other clinicopathological parameters. There was a correlation between TET2 expression and the localization of the tumors. 5-mC expression was not associated with any clinicopathological parameters. Conclusions: We identified a significant 5-hmC loss in conjunctival melanoma similar to cutaneous melanoma. This loss may possibly be attributed to TET2 loss or IDH1 mutations. 5-hmC loss in conjunctival melanoma may help in the differential diagnosis between atypical conjunctival nevus and conjunctival melanoma. |
format | Online Article Text |
id | pubmed-8293194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82931942021-07-22 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma Stahl, Alexandre Riggi, Nicolo Nardou, Katya Nicolas, Michael Kaya, Gurkan Moulin, Alexandre Dermatopathology (Basel) Article Aims: Conjunctival and cutaneous melanoma partially share similar clinical and molecular backgrounds. As 5-hydroxymethylcytosine (5-hmC) loss has been demonstrated in cutaneous melanoma, we decided to assess if similar changes were occurring in conjunctival melanoma. Methods: 5-methylcytosine (5-mC), 5-hmC and TET2 were respectively identified by immunohistochemistry and RNA ISH in 40 conjunctival nevi and 37 conjunctival melanomas. Clinicopathological correlations were established. Results: 5-mC, TET2 and 5-hmC were respectively identified in 67.5%, 95% and 100% of conjunctival nevi and in 81.1%, 35.1% and 54% of conjunctival melanomas. A significant 5-hmC and TET2 loss was identified in conjunctival melanoma comparing to nevus, as well as a significant correlation between TET2 and 5-hmC expression. In the melanomas, 5-hmC expression was only significantly associated with local lymphatic invasion, but not with other clinicopathological parameters. There was a correlation between TET2 expression and the localization of the tumors. 5-mC expression was not associated with any clinicopathological parameters. Conclusions: We identified a significant 5-hmC loss in conjunctival melanoma similar to cutaneous melanoma. This loss may possibly be attributed to TET2 loss or IDH1 mutations. 5-hmC loss in conjunctival melanoma may help in the differential diagnosis between atypical conjunctival nevus and conjunctival melanoma. MDPI 2021-06-05 /pmc/articles/PMC8293194/ /pubmed/34198758 http://dx.doi.org/10.3390/dermatopathology8020023 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Stahl, Alexandre Riggi, Nicolo Nardou, Katya Nicolas, Michael Kaya, Gurkan Moulin, Alexandre 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma |
title | 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma |
title_full | 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma |
title_fullStr | 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma |
title_full_unstemmed | 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma |
title_short | 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma |
title_sort | 5-hydroxymethylcytosine loss in conjunctival melanoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293194/ https://www.ncbi.nlm.nih.gov/pubmed/34198758 http://dx.doi.org/10.3390/dermatopathology8020023 |
work_keys_str_mv | AT stahlalexandre 5hydroxymethylcytosinelossinconjunctivalmelanoma AT rigginicolo 5hydroxymethylcytosinelossinconjunctivalmelanoma AT nardoukatya 5hydroxymethylcytosinelossinconjunctivalmelanoma AT nicolasmichael 5hydroxymethylcytosinelossinconjunctivalmelanoma AT kayagurkan 5hydroxymethylcytosinelossinconjunctivalmelanoma AT moulinalexandre 5hydroxymethylcytosinelossinconjunctivalmelanoma |