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5-Hydroxymethylcytosine Loss in Conjunctival Melanoma

Aims: Conjunctival and cutaneous melanoma partially share similar clinical and molecular backgrounds. As 5-hydroxymethylcytosine (5-hmC) loss has been demonstrated in cutaneous melanoma, we decided to assess if similar changes were occurring in conjunctival melanoma. Methods: 5-methylcytosine (5-mC)...

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Autores principales: Stahl, Alexandre, Riggi, Nicolo, Nardou, Katya, Nicolas, Michael, Kaya, Gurkan, Moulin, Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293194/
https://www.ncbi.nlm.nih.gov/pubmed/34198758
http://dx.doi.org/10.3390/dermatopathology8020023
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author Stahl, Alexandre
Riggi, Nicolo
Nardou, Katya
Nicolas, Michael
Kaya, Gurkan
Moulin, Alexandre
author_facet Stahl, Alexandre
Riggi, Nicolo
Nardou, Katya
Nicolas, Michael
Kaya, Gurkan
Moulin, Alexandre
author_sort Stahl, Alexandre
collection PubMed
description Aims: Conjunctival and cutaneous melanoma partially share similar clinical and molecular backgrounds. As 5-hydroxymethylcytosine (5-hmC) loss has been demonstrated in cutaneous melanoma, we decided to assess if similar changes were occurring in conjunctival melanoma. Methods: 5-methylcytosine (5-mC), 5-hmC and TET2 were respectively identified by immunohistochemistry and RNA ISH in 40 conjunctival nevi and 37 conjunctival melanomas. Clinicopathological correlations were established. Results: 5-mC, TET2 and 5-hmC were respectively identified in 67.5%, 95% and 100% of conjunctival nevi and in 81.1%, 35.1% and 54% of conjunctival melanomas. A significant 5-hmC and TET2 loss was identified in conjunctival melanoma comparing to nevus, as well as a significant correlation between TET2 and 5-hmC expression. In the melanomas, 5-hmC expression was only significantly associated with local lymphatic invasion, but not with other clinicopathological parameters. There was a correlation between TET2 expression and the localization of the tumors. 5-mC expression was not associated with any clinicopathological parameters. Conclusions: We identified a significant 5-hmC loss in conjunctival melanoma similar to cutaneous melanoma. This loss may possibly be attributed to TET2 loss or IDH1 mutations. 5-hmC loss in conjunctival melanoma may help in the differential diagnosis between atypical conjunctival nevus and conjunctival melanoma.
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spelling pubmed-82931942021-07-22 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma Stahl, Alexandre Riggi, Nicolo Nardou, Katya Nicolas, Michael Kaya, Gurkan Moulin, Alexandre Dermatopathology (Basel) Article Aims: Conjunctival and cutaneous melanoma partially share similar clinical and molecular backgrounds. As 5-hydroxymethylcytosine (5-hmC) loss has been demonstrated in cutaneous melanoma, we decided to assess if similar changes were occurring in conjunctival melanoma. Methods: 5-methylcytosine (5-mC), 5-hmC and TET2 were respectively identified by immunohistochemistry and RNA ISH in 40 conjunctival nevi and 37 conjunctival melanomas. Clinicopathological correlations were established. Results: 5-mC, TET2 and 5-hmC were respectively identified in 67.5%, 95% and 100% of conjunctival nevi and in 81.1%, 35.1% and 54% of conjunctival melanomas. A significant 5-hmC and TET2 loss was identified in conjunctival melanoma comparing to nevus, as well as a significant correlation between TET2 and 5-hmC expression. In the melanomas, 5-hmC expression was only significantly associated with local lymphatic invasion, but not with other clinicopathological parameters. There was a correlation between TET2 expression and the localization of the tumors. 5-mC expression was not associated with any clinicopathological parameters. Conclusions: We identified a significant 5-hmC loss in conjunctival melanoma similar to cutaneous melanoma. This loss may possibly be attributed to TET2 loss or IDH1 mutations. 5-hmC loss in conjunctival melanoma may help in the differential diagnosis between atypical conjunctival nevus and conjunctival melanoma. MDPI 2021-06-05 /pmc/articles/PMC8293194/ /pubmed/34198758 http://dx.doi.org/10.3390/dermatopathology8020023 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stahl, Alexandre
Riggi, Nicolo
Nardou, Katya
Nicolas, Michael
Kaya, Gurkan
Moulin, Alexandre
5-Hydroxymethylcytosine Loss in Conjunctival Melanoma
title 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma
title_full 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma
title_fullStr 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma
title_full_unstemmed 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma
title_short 5-Hydroxymethylcytosine Loss in Conjunctival Melanoma
title_sort 5-hydroxymethylcytosine loss in conjunctival melanoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293194/
https://www.ncbi.nlm.nih.gov/pubmed/34198758
http://dx.doi.org/10.3390/dermatopathology8020023
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