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Discrimination of Cancer Stem Cell Markers ALDH1A1, BCL11B, BMI-1, and CD44 in Different Tissues of HNSCC Patients

Cancer stem cells (CSCs) are accountable for the progress of head and neck squamous cell carcinoma (HNSCC). This exploratory study evaluated the expression of molecular CSC markers in different tissues of HNSCC patients. Tissue specimens of primary tumor, lymph node metastases and macroscopically he...

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Autores principales: Sharaf, Kariem, Lechner, Axel, Haider, Stefan P., Wiebringhaus, Robert, Walz, Christoph, Kranz, Gisela, Canis, Martin, Haubner, Frank, Gires, Olivier, Baumeister, Philipp
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293237/
https://www.ncbi.nlm.nih.gov/pubmed/34287293
http://dx.doi.org/10.3390/curroncol28040241
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author Sharaf, Kariem
Lechner, Axel
Haider, Stefan P.
Wiebringhaus, Robert
Walz, Christoph
Kranz, Gisela
Canis, Martin
Haubner, Frank
Gires, Olivier
Baumeister, Philipp
author_facet Sharaf, Kariem
Lechner, Axel
Haider, Stefan P.
Wiebringhaus, Robert
Walz, Christoph
Kranz, Gisela
Canis, Martin
Haubner, Frank
Gires, Olivier
Baumeister, Philipp
author_sort Sharaf, Kariem
collection PubMed
description Cancer stem cells (CSCs) are accountable for the progress of head and neck squamous cell carcinoma (HNSCC). This exploratory study evaluated the expression of molecular CSC markers in different tissues of HNSCC patients. Tissue specimens of primary tumor, lymph node metastases and macroscopically healthy mucosa of 12 consecutive HNSCC patients, that were treated with surgery and adjuvant radio(chemo)therapy upon indication, were collected. Samples were assessed for the expression of p16 as a surrogate for HPV-related disease and different molecular stem cell markers (ALDH1A1, BCL11B, BMI-1, and CD44). In the cohort, seven patients had HPV-related HNSCC; six thereof were oropharyngeal squamous cell carcinoma. While expression of BMI-1 and BCL11B was significantly lower in healthy mucosa than both tumor and lymph node metastasis, there were no differences between tumor and lymph node metastasis. In the HPV-positive sub-cohort, these differences remained significant for BMI-1. However, no significant differences in these three tissues were found for ALDH1A1 and CD44. In conclusion, this exploratory study shows that CSC markers BMI-1 and BCL11B discriminate between healthy and cancerous tissue, whereas ALDH1A1 and CD44 were expressed to a comparable extent in healthy mucosa and cancerous tissues.
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spelling pubmed-82932372021-07-22 Discrimination of Cancer Stem Cell Markers ALDH1A1, BCL11B, BMI-1, and CD44 in Different Tissues of HNSCC Patients Sharaf, Kariem Lechner, Axel Haider, Stefan P. Wiebringhaus, Robert Walz, Christoph Kranz, Gisela Canis, Martin Haubner, Frank Gires, Olivier Baumeister, Philipp Curr Oncol Article Cancer stem cells (CSCs) are accountable for the progress of head and neck squamous cell carcinoma (HNSCC). This exploratory study evaluated the expression of molecular CSC markers in different tissues of HNSCC patients. Tissue specimens of primary tumor, lymph node metastases and macroscopically healthy mucosa of 12 consecutive HNSCC patients, that were treated with surgery and adjuvant radio(chemo)therapy upon indication, were collected. Samples were assessed for the expression of p16 as a surrogate for HPV-related disease and different molecular stem cell markers (ALDH1A1, BCL11B, BMI-1, and CD44). In the cohort, seven patients had HPV-related HNSCC; six thereof were oropharyngeal squamous cell carcinoma. While expression of BMI-1 and BCL11B was significantly lower in healthy mucosa than both tumor and lymph node metastasis, there were no differences between tumor and lymph node metastasis. In the HPV-positive sub-cohort, these differences remained significant for BMI-1. However, no significant differences in these three tissues were found for ALDH1A1 and CD44. In conclusion, this exploratory study shows that CSC markers BMI-1 and BCL11B discriminate between healthy and cancerous tissue, whereas ALDH1A1 and CD44 were expressed to a comparable extent in healthy mucosa and cancerous tissues. MDPI 2021-07-19 /pmc/articles/PMC8293237/ /pubmed/34287293 http://dx.doi.org/10.3390/curroncol28040241 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sharaf, Kariem
Lechner, Axel
Haider, Stefan P.
Wiebringhaus, Robert
Walz, Christoph
Kranz, Gisela
Canis, Martin
Haubner, Frank
Gires, Olivier
Baumeister, Philipp
Discrimination of Cancer Stem Cell Markers ALDH1A1, BCL11B, BMI-1, and CD44 in Different Tissues of HNSCC Patients
title Discrimination of Cancer Stem Cell Markers ALDH1A1, BCL11B, BMI-1, and CD44 in Different Tissues of HNSCC Patients
title_full Discrimination of Cancer Stem Cell Markers ALDH1A1, BCL11B, BMI-1, and CD44 in Different Tissues of HNSCC Patients
title_fullStr Discrimination of Cancer Stem Cell Markers ALDH1A1, BCL11B, BMI-1, and CD44 in Different Tissues of HNSCC Patients
title_full_unstemmed Discrimination of Cancer Stem Cell Markers ALDH1A1, BCL11B, BMI-1, and CD44 in Different Tissues of HNSCC Patients
title_short Discrimination of Cancer Stem Cell Markers ALDH1A1, BCL11B, BMI-1, and CD44 in Different Tissues of HNSCC Patients
title_sort discrimination of cancer stem cell markers aldh1a1, bcl11b, bmi-1, and cd44 in different tissues of hnscc patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293237/
https://www.ncbi.nlm.nih.gov/pubmed/34287293
http://dx.doi.org/10.3390/curroncol28040241
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