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Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development
Wnt signaling regulates cell fate decisions in diverse contexts during development, and loss of Wnt signaling in the cranial mesenchyme results in a robust and binary cell fate switch from cranial bone to ectopic cartilage. The Extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) and Wnt s...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293402/ https://www.ncbi.nlm.nih.gov/pubmed/34199092 http://dx.doi.org/10.3390/jdb9030023 |
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author | Ibarra, Beatriz A Machen, Cody Atit, Radhika P. |
author_facet | Ibarra, Beatriz A Machen, Cody Atit, Radhika P. |
author_sort | Ibarra, Beatriz A |
collection | PubMed |
description | Wnt signaling regulates cell fate decisions in diverse contexts during development, and loss of Wnt signaling in the cranial mesenchyme results in a robust and binary cell fate switch from cranial bone to ectopic cartilage. The Extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) and Wnt signaling pathways are activated during calvarial osteoblast cell fate selection. Here, we test the hypothesis that ERK signaling is a mediator of Wnt-dependent cell fate decisions in the cranial mesenchyme. First, we show that loss of Erk1/2 in the cranial mesenchyme results in a diminished domain of osteoblast marker expression and increased expression of cartilage fate markers and ectopic cartilage formation in the frontal bone primordia. Second, we show that mesenchyme Wnt/β-catenin signaling and Wntless are required for ERK activation in calvarial osteoblasts. Third, we demonstrate that Wnt and ERK signaling pathways function together to repress SOX9 expression in mouse cranial mesenchyme. Our results demonstrate an interaction between the Wnt and ERK signaling pathways in regulating lineage selection in a subset of calvarial cells and provide new insights into Wnt-dependent cell fate decisions. |
format | Online Article Text |
id | pubmed-8293402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82934022021-07-22 Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development Ibarra, Beatriz A Machen, Cody Atit, Radhika P. J Dev Biol Article Wnt signaling regulates cell fate decisions in diverse contexts during development, and loss of Wnt signaling in the cranial mesenchyme results in a robust and binary cell fate switch from cranial bone to ectopic cartilage. The Extracellular signal-regulated protein kinase 1 and 2 (ERK1/2) and Wnt signaling pathways are activated during calvarial osteoblast cell fate selection. Here, we test the hypothesis that ERK signaling is a mediator of Wnt-dependent cell fate decisions in the cranial mesenchyme. First, we show that loss of Erk1/2 in the cranial mesenchyme results in a diminished domain of osteoblast marker expression and increased expression of cartilage fate markers and ectopic cartilage formation in the frontal bone primordia. Second, we show that mesenchyme Wnt/β-catenin signaling and Wntless are required for ERK activation in calvarial osteoblasts. Third, we demonstrate that Wnt and ERK signaling pathways function together to repress SOX9 expression in mouse cranial mesenchyme. Our results demonstrate an interaction between the Wnt and ERK signaling pathways in regulating lineage selection in a subset of calvarial cells and provide new insights into Wnt-dependent cell fate decisions. MDPI 2021-06-27 /pmc/articles/PMC8293402/ /pubmed/34199092 http://dx.doi.org/10.3390/jdb9030023 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ibarra, Beatriz A Machen, Cody Atit, Radhika P. Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development |
title | Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development |
title_full | Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development |
title_fullStr | Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development |
title_full_unstemmed | Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development |
title_short | Wnt-Dependent Activation of ERK Mediates Repression of Chondrocyte Fate during Calvarial Development |
title_sort | wnt-dependent activation of erk mediates repression of chondrocyte fate during calvarial development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293402/ https://www.ncbi.nlm.nih.gov/pubmed/34199092 http://dx.doi.org/10.3390/jdb9030023 |
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