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Phlebotomine sand fly survey in the Republic of Moldova: species composition, distribution and host preferences
BACKGROUND: Phlebotomine sand flies (Diptera: Psychodiae) in the Republic of Moldova have been understudied for decades. Our study provides a first update on their occurrence, species composition and bloodmeal sources after 50 years. METHODS: During 5 seasons (2013–2017), 58 localities from 20 regio...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293551/ https://www.ncbi.nlm.nih.gov/pubmed/34289883 http://dx.doi.org/10.1186/s13071-021-04858-4 |
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author | Șuleșco, Tatiana Erisoz Kasap, Ozge Halada, Petr Oğuz, Gizem Rusnac, Dimian Gresova, Marketa Alten, Bulent Volf, Petr Dvorak, Vit |
author_facet | Șuleșco, Tatiana Erisoz Kasap, Ozge Halada, Petr Oğuz, Gizem Rusnac, Dimian Gresova, Marketa Alten, Bulent Volf, Petr Dvorak, Vit |
author_sort | Șuleșco, Tatiana |
collection | PubMed |
description | BACKGROUND: Phlebotomine sand flies (Diptera: Psychodiae) in the Republic of Moldova have been understudied for decades. Our study provides a first update on their occurrence, species composition and bloodmeal sources after 50 years. METHODS: During 5 seasons (2013–2017), 58 localities from 20 regions were surveyed for presence of sand flies using CDC light traps and manual aspirators. Species identification was done by a combination of morphological and molecular approaches (DNA barcoding, MALDI-TOF MS protein profiling). In engorged females, host blood was identified by three molecular techniques (RFLP, cytb sequencing and MALDI-TOF peptide mass mapping). Population structure of most abundant species was studied by cox1 haplotyping; phylogenetic analyses of ITS2 and cox1 genetic markers were used to resolve relationships of other detected species. RESULTS: In total, 793 sand flies were collected at 30 (51.7%) localities from 12 regions of Moldova. Three species were identified by an integrative morphological and molecular approach: Phlebotomus papatasi, P. perfiliewi and Phlebotomus sp. (Adlerius), the first being the most abundant and widespread, markedly anthropophilic based on bloodmeal analyses, occurring also indoors and showing low population structure with only five haplotypes of cox1 detected. Distinct morphological and molecular characters of Phlebotomus sp. (Adlerius) specimens suggest the presence of a yet undescribed species. CONCLUSIONS: Our study revealed the presence of stable sand fly populations of three species in Moldova that represent a biting nuisance as well as a potential threat of pathogen transmission and shall be further studied. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-021-04858-4. |
format | Online Article Text |
id | pubmed-8293551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-82935512021-07-21 Phlebotomine sand fly survey in the Republic of Moldova: species composition, distribution and host preferences Șuleșco, Tatiana Erisoz Kasap, Ozge Halada, Petr Oğuz, Gizem Rusnac, Dimian Gresova, Marketa Alten, Bulent Volf, Petr Dvorak, Vit Parasit Vectors Research BACKGROUND: Phlebotomine sand flies (Diptera: Psychodiae) in the Republic of Moldova have been understudied for decades. Our study provides a first update on their occurrence, species composition and bloodmeal sources after 50 years. METHODS: During 5 seasons (2013–2017), 58 localities from 20 regions were surveyed for presence of sand flies using CDC light traps and manual aspirators. Species identification was done by a combination of morphological and molecular approaches (DNA barcoding, MALDI-TOF MS protein profiling). In engorged females, host blood was identified by three molecular techniques (RFLP, cytb sequencing and MALDI-TOF peptide mass mapping). Population structure of most abundant species was studied by cox1 haplotyping; phylogenetic analyses of ITS2 and cox1 genetic markers were used to resolve relationships of other detected species. RESULTS: In total, 793 sand flies were collected at 30 (51.7%) localities from 12 regions of Moldova. Three species were identified by an integrative morphological and molecular approach: Phlebotomus papatasi, P. perfiliewi and Phlebotomus sp. (Adlerius), the first being the most abundant and widespread, markedly anthropophilic based on bloodmeal analyses, occurring also indoors and showing low population structure with only five haplotypes of cox1 detected. Distinct morphological and molecular characters of Phlebotomus sp. (Adlerius) specimens suggest the presence of a yet undescribed species. CONCLUSIONS: Our study revealed the presence of stable sand fly populations of three species in Moldova that represent a biting nuisance as well as a potential threat of pathogen transmission and shall be further studied. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-021-04858-4. BioMed Central 2021-07-21 /pmc/articles/PMC8293551/ /pubmed/34289883 http://dx.doi.org/10.1186/s13071-021-04858-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Șuleșco, Tatiana Erisoz Kasap, Ozge Halada, Petr Oğuz, Gizem Rusnac, Dimian Gresova, Marketa Alten, Bulent Volf, Petr Dvorak, Vit Phlebotomine sand fly survey in the Republic of Moldova: species composition, distribution and host preferences |
title | Phlebotomine sand fly survey in the Republic of Moldova: species composition, distribution and host preferences |
title_full | Phlebotomine sand fly survey in the Republic of Moldova: species composition, distribution and host preferences |
title_fullStr | Phlebotomine sand fly survey in the Republic of Moldova: species composition, distribution and host preferences |
title_full_unstemmed | Phlebotomine sand fly survey in the Republic of Moldova: species composition, distribution and host preferences |
title_short | Phlebotomine sand fly survey in the Republic of Moldova: species composition, distribution and host preferences |
title_sort | phlebotomine sand fly survey in the republic of moldova: species composition, distribution and host preferences |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293551/ https://www.ncbi.nlm.nih.gov/pubmed/34289883 http://dx.doi.org/10.1186/s13071-021-04858-4 |
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