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Taming the Huntington’s Disease Proteome: What Have We Learned?

Mass spectrometry (MS) is a physical technique used to identify specific chemicals and molecules by precise analysis of their mass and charge; this technology has been adapted for biological sciences applications. Investigators have used MS to identify differential expressions of proteins in Hunting...

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Detalles Bibliográficos
Autores principales: Seeley, Connor, Kegel-Gleason, Kimberly B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293646/
https://www.ncbi.nlm.nih.gov/pubmed/33998547
http://dx.doi.org/10.3233/JHD-200465
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author Seeley, Connor
Kegel-Gleason, Kimberly B.
author_facet Seeley, Connor
Kegel-Gleason, Kimberly B.
author_sort Seeley, Connor
collection PubMed
description Mass spectrometry (MS) is a physical technique used to identify specific chemicals and molecules by precise analysis of their mass and charge; this technology has been adapted for biological sciences applications. Investigators have used MS to identify differential expressions of proteins in Huntington’s disease (HD), to discover Huntingtin (HTT) interacting proteins and to analyze HTT proteoforms. Using systems biology and computational approaches, data from MS screens have been leveraged to find differentially expressed pathways. This review summarizes the data from most of the MS studies done in the HD field in the last 20 years and compares it to the protein data reported before the use of MS technology. The MS results validate early findings in the field such as differential expression of PDE10a and DARPP-32 and identify new changes. We offer a perspective on the MS approach in HD, particularly for identification of disease pathways, the challenges in interpreting data across different studies, and its application to protein studies moving forward.
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spelling pubmed-82936462021-08-05 Taming the Huntington’s Disease Proteome: What Have We Learned? Seeley, Connor Kegel-Gleason, Kimberly B. J Huntingtons Dis Review Mass spectrometry (MS) is a physical technique used to identify specific chemicals and molecules by precise analysis of their mass and charge; this technology has been adapted for biological sciences applications. Investigators have used MS to identify differential expressions of proteins in Huntington’s disease (HD), to discover Huntingtin (HTT) interacting proteins and to analyze HTT proteoforms. Using systems biology and computational approaches, data from MS screens have been leveraged to find differentially expressed pathways. This review summarizes the data from most of the MS studies done in the HD field in the last 20 years and compares it to the protein data reported before the use of MS technology. The MS results validate early findings in the field such as differential expression of PDE10a and DARPP-32 and identify new changes. We offer a perspective on the MS approach in HD, particularly for identification of disease pathways, the challenges in interpreting data across different studies, and its application to protein studies moving forward. IOS Press 2021-06-09 /pmc/articles/PMC8293646/ /pubmed/33998547 http://dx.doi.org/10.3233/JHD-200465 Text en © 2021 – The authors. Published by IOS Press https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial (CC BY-NC 4.0) License (https://creativecommons.org/licenses/by-nc/4.0/) , which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Seeley, Connor
Kegel-Gleason, Kimberly B.
Taming the Huntington’s Disease Proteome: What Have We Learned?
title Taming the Huntington’s Disease Proteome: What Have We Learned?
title_full Taming the Huntington’s Disease Proteome: What Have We Learned?
title_fullStr Taming the Huntington’s Disease Proteome: What Have We Learned?
title_full_unstemmed Taming the Huntington’s Disease Proteome: What Have We Learned?
title_short Taming the Huntington’s Disease Proteome: What Have We Learned?
title_sort taming the huntington’s disease proteome: what have we learned?
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293646/
https://www.ncbi.nlm.nih.gov/pubmed/33998547
http://dx.doi.org/10.3233/JHD-200465
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