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Performance Comparison of Xpert HIV-1 Viral Load Assay and Roche Taqman and Abbott M2000 RT in Bamako, Mali

BACKGROUND: Routine monitoring of HIV-1 Viral Load (VL) is important in patients on Antiretroviral Therapy (ART) management. Access to HIV VL remains a challenge in resource-limited settings, especially in rural areas. Universal access to VL requires more simplified and less restrictive alternatives...

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Autores principales: Kone, Bourahima, Goita, Drissa, Dolo, Oumar, Traore, Daouda, Sogoba, Dramane, Somboro, Amadou, Sanogo, Moumine, Somboro, Anou M, Coulibaly, Nadie, Sanogo, Alou, Diarra, Zoumana, Traore, Madou, Maiga, Almoustapha I, Baya, Bocar, Sarro, Yeya Dit Sadio, Diarra, Bassirou, Kone, Amadou, Diallo, Dramane, Dabitao, Djeneba, Holl, Jane L., Belson, Michael, Dao, Sounkalo, Murphy, Robert L., Diakite, Mahamadou, Diallo, Souleymane, Doumbia, Seydou, Maiga, Mamoudou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294115/
https://www.ncbi.nlm.nih.gov/pubmed/34295571
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author Kone, Bourahima
Goita, Drissa
Dolo, Oumar
Traore, Daouda
Sogoba, Dramane
Somboro, Amadou
Sanogo, Moumine
Somboro, Anou M
Coulibaly, Nadie
Sanogo, Alou
Diarra, Zoumana
Traore, Madou
Maiga, Almoustapha I
Baya, Bocar
Sarro, Yeya Dit Sadio
Diarra, Bassirou
Kone, Amadou
Diallo, Dramane
Dabitao, Djeneba
Holl, Jane L.
Belson, Michael
Dao, Sounkalo
Murphy, Robert L.
Diakite, Mahamadou
Diallo, Souleymane
Doumbia, Seydou
Maiga, Mamoudou
author_facet Kone, Bourahima
Goita, Drissa
Dolo, Oumar
Traore, Daouda
Sogoba, Dramane
Somboro, Amadou
Sanogo, Moumine
Somboro, Anou M
Coulibaly, Nadie
Sanogo, Alou
Diarra, Zoumana
Traore, Madou
Maiga, Almoustapha I
Baya, Bocar
Sarro, Yeya Dit Sadio
Diarra, Bassirou
Kone, Amadou
Diallo, Dramane
Dabitao, Djeneba
Holl, Jane L.
Belson, Michael
Dao, Sounkalo
Murphy, Robert L.
Diakite, Mahamadou
Diallo, Souleymane
Doumbia, Seydou
Maiga, Mamoudou
author_sort Kone, Bourahima
collection PubMed
description BACKGROUND: Routine monitoring of HIV-1 Viral Load (VL) is important in patients on Antiretroviral Therapy (ART) management. Access to HIV VL remains a challenge in resource-limited settings, especially in rural areas. Universal access to VL requires more simplified and less restrictive alternatives to current conventional VL methods. The objective of this study was to evaluate the performance of the new rapid (2-hour turnaround time) Xpert HIV-1VL technique compared to Roche TaqMan and Abbott RT m2000 for HIV-1 RNA quantification in HIV- infected patients. STUDY DESIGN: We conducted a cross-sectional study in patients seen for routine VL monitoring between August and November 2018 in a HIV care site in Bamako. The performance of the Xpert HIV-1 VL assay was evaluated against the Roche TaqMan assay and Abbott m2000 RT assay. Performance, utility and reliability/reproducibility were verified using accuracy, sensitivity, specificity, positive and negative predictive values, Diagnostic Odds Ratio (DOR), Kappa coefficient, Pearson correlation coefficient, and Bland-Altman analysis. RESULTS: The Xpert assay compared well with the two current referral assays (Roche TaqMan and Abbott m2000 RT assays). Compared to Roche TaqMan assay the sensitivity was 93.10%, specificity (97.01%) and accuracy (95.20%), the correlation coefficient of Pearson (r) was 0.98 (p <0.01). Bland-Altman analysis showed a mean difference of 0.18 log10 cp/mL; (Standard Deviation) SD=0.33. Compared to the Abbott m2000 RT, the sensitivity, the specificity and the accuracy were respectively 93.44%; 92% and 92.65%. The Xpert HIV-1 VL assay showed a good correlation with a correlation coefficient of Pearson, r=0.99 (p <0.001). The overall mean difference in the HIV-1 VL values obtained by Xpert HIV-1 VL and Abbott m2000 RT assays was 0.08 log10 cp/mL; SD=0.30. CONCLUSION: Xpert HIV-1 VL showed a good performance compared to Roche TaqMan and Abbott m2000 RT. With the rapid test results (less than 2 h) and ease of testing individual specimens, the Xpert HIV-1 VL assay could be an effective alternative for HIV VL monitoring in resource-limited settings.
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spelling pubmed-82941152021-07-21 Performance Comparison of Xpert HIV-1 Viral Load Assay and Roche Taqman and Abbott M2000 RT in Bamako, Mali Kone, Bourahima Goita, Drissa Dolo, Oumar Traore, Daouda Sogoba, Dramane Somboro, Amadou Sanogo, Moumine Somboro, Anou M Coulibaly, Nadie Sanogo, Alou Diarra, Zoumana Traore, Madou Maiga, Almoustapha I Baya, Bocar Sarro, Yeya Dit Sadio Diarra, Bassirou Kone, Amadou Diallo, Dramane Dabitao, Djeneba Holl, Jane L. Belson, Michael Dao, Sounkalo Murphy, Robert L. Diakite, Mahamadou Diallo, Souleymane Doumbia, Seydou Maiga, Mamoudou J AIDS Clin Res Article BACKGROUND: Routine monitoring of HIV-1 Viral Load (VL) is important in patients on Antiretroviral Therapy (ART) management. Access to HIV VL remains a challenge in resource-limited settings, especially in rural areas. Universal access to VL requires more simplified and less restrictive alternatives to current conventional VL methods. The objective of this study was to evaluate the performance of the new rapid (2-hour turnaround time) Xpert HIV-1VL technique compared to Roche TaqMan and Abbott RT m2000 for HIV-1 RNA quantification in HIV- infected patients. STUDY DESIGN: We conducted a cross-sectional study in patients seen for routine VL monitoring between August and November 2018 in a HIV care site in Bamako. The performance of the Xpert HIV-1 VL assay was evaluated against the Roche TaqMan assay and Abbott m2000 RT assay. Performance, utility and reliability/reproducibility were verified using accuracy, sensitivity, specificity, positive and negative predictive values, Diagnostic Odds Ratio (DOR), Kappa coefficient, Pearson correlation coefficient, and Bland-Altman analysis. RESULTS: The Xpert assay compared well with the two current referral assays (Roche TaqMan and Abbott m2000 RT assays). Compared to Roche TaqMan assay the sensitivity was 93.10%, specificity (97.01%) and accuracy (95.20%), the correlation coefficient of Pearson (r) was 0.98 (p <0.01). Bland-Altman analysis showed a mean difference of 0.18 log10 cp/mL; (Standard Deviation) SD=0.33. Compared to the Abbott m2000 RT, the sensitivity, the specificity and the accuracy were respectively 93.44%; 92% and 92.65%. The Xpert HIV-1 VL assay showed a good correlation with a correlation coefficient of Pearson, r=0.99 (p <0.001). The overall mean difference in the HIV-1 VL values obtained by Xpert HIV-1 VL and Abbott m2000 RT assays was 0.08 log10 cp/mL; SD=0.30. CONCLUSION: Xpert HIV-1 VL showed a good performance compared to Roche TaqMan and Abbott m2000 RT. With the rapid test results (less than 2 h) and ease of testing individual specimens, the Xpert HIV-1 VL assay could be an effective alternative for HIV VL monitoring in resource-limited settings. 2020 2020-07-30 /pmc/articles/PMC8294115/ /pubmed/34295571 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Kone, Bourahima
Goita, Drissa
Dolo, Oumar
Traore, Daouda
Sogoba, Dramane
Somboro, Amadou
Sanogo, Moumine
Somboro, Anou M
Coulibaly, Nadie
Sanogo, Alou
Diarra, Zoumana
Traore, Madou
Maiga, Almoustapha I
Baya, Bocar
Sarro, Yeya Dit Sadio
Diarra, Bassirou
Kone, Amadou
Diallo, Dramane
Dabitao, Djeneba
Holl, Jane L.
Belson, Michael
Dao, Sounkalo
Murphy, Robert L.
Diakite, Mahamadou
Diallo, Souleymane
Doumbia, Seydou
Maiga, Mamoudou
Performance Comparison of Xpert HIV-1 Viral Load Assay and Roche Taqman and Abbott M2000 RT in Bamako, Mali
title Performance Comparison of Xpert HIV-1 Viral Load Assay and Roche Taqman and Abbott M2000 RT in Bamako, Mali
title_full Performance Comparison of Xpert HIV-1 Viral Load Assay and Roche Taqman and Abbott M2000 RT in Bamako, Mali
title_fullStr Performance Comparison of Xpert HIV-1 Viral Load Assay and Roche Taqman and Abbott M2000 RT in Bamako, Mali
title_full_unstemmed Performance Comparison of Xpert HIV-1 Viral Load Assay and Roche Taqman and Abbott M2000 RT in Bamako, Mali
title_short Performance Comparison of Xpert HIV-1 Viral Load Assay and Roche Taqman and Abbott M2000 RT in Bamako, Mali
title_sort performance comparison of xpert hiv-1 viral load assay and roche taqman and abbott m2000 rt in bamako, mali
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294115/
https://www.ncbi.nlm.nih.gov/pubmed/34295571
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