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Ribosome as a Translocase and Helicase
During protein synthesis, ribosome moves along mRNA to decode one codon after the other. Ribosome translocation is induced by a universally conserved protein, elongation factor G (EF-G) in bacteria and elongation factor 2 (EF-2) in eukaryotes. EF-G-induced translocation results in unwinding of the i...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pleiades Publishing
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294220/ https://www.ncbi.nlm.nih.gov/pubmed/34488575 http://dx.doi.org/10.1134/S0006297921080095 |
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author | Bao, Chen Ermolenko, Dmitri N. |
author_facet | Bao, Chen Ermolenko, Dmitri N. |
author_sort | Bao, Chen |
collection | PubMed |
description | During protein synthesis, ribosome moves along mRNA to decode one codon after the other. Ribosome translocation is induced by a universally conserved protein, elongation factor G (EF-G) in bacteria and elongation factor 2 (EF-2) in eukaryotes. EF-G-induced translocation results in unwinding of the intramolecular secondary structures of mRNA by three base pairs at a time that renders the translating ribosome a processive helicase. Professor Alexander Sergeevich Spirin has made numerous seminal contributions to understanding the molecular mechanism of translocation. Here, we review Spirin’s insights into the ribosomal translocation and recent advances in the field that stemmed from Spirin’s pioneering work. We also discuss key remaining challenges in studies of translocase and helicase activities of the ribosome. |
format | Online Article Text |
id | pubmed-8294220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Pleiades Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-82942202021-07-21 Ribosome as a Translocase and Helicase Bao, Chen Ermolenko, Dmitri N. Biochemistry (Mosc) Review During protein synthesis, ribosome moves along mRNA to decode one codon after the other. Ribosome translocation is induced by a universally conserved protein, elongation factor G (EF-G) in bacteria and elongation factor 2 (EF-2) in eukaryotes. EF-G-induced translocation results in unwinding of the intramolecular secondary structures of mRNA by three base pairs at a time that renders the translating ribosome a processive helicase. Professor Alexander Sergeevich Spirin has made numerous seminal contributions to understanding the molecular mechanism of translocation. Here, we review Spirin’s insights into the ribosomal translocation and recent advances in the field that stemmed from Spirin’s pioneering work. We also discuss key remaining challenges in studies of translocase and helicase activities of the ribosome. Pleiades Publishing 2021-07-21 2021 /pmc/articles/PMC8294220/ /pubmed/34488575 http://dx.doi.org/10.1134/S0006297921080095 Text en © Pleiades Publishing, Ltd. 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Review Bao, Chen Ermolenko, Dmitri N. Ribosome as a Translocase and Helicase |
title | Ribosome as a Translocase and Helicase |
title_full | Ribosome as a Translocase and Helicase |
title_fullStr | Ribosome as a Translocase and Helicase |
title_full_unstemmed | Ribosome as a Translocase and Helicase |
title_short | Ribosome as a Translocase and Helicase |
title_sort | ribosome as a translocase and helicase |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294220/ https://www.ncbi.nlm.nih.gov/pubmed/34488575 http://dx.doi.org/10.1134/S0006297921080095 |
work_keys_str_mv | AT baochen ribosomeasatranslocaseandhelicase AT ermolenkodmitrin ribosomeasatranslocaseandhelicase |