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Dynamics of transcriptional and post-transcriptional regulation
Despite gene expression programs being notoriously complex, RNA abundance is usually assumed as a proxy for transcriptional activity. Recently developed approaches, able to disentangle transcriptional and post-transcriptional regulatory processes, have revealed a more complex scenario. It is now pos...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294512/ https://www.ncbi.nlm.nih.gov/pubmed/33348360 http://dx.doi.org/10.1093/bib/bbaa389 |
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author | Furlan, Mattia de Pretis, Stefano Pelizzola, Mattia |
author_facet | Furlan, Mattia de Pretis, Stefano Pelizzola, Mattia |
author_sort | Furlan, Mattia |
collection | PubMed |
description | Despite gene expression programs being notoriously complex, RNA abundance is usually assumed as a proxy for transcriptional activity. Recently developed approaches, able to disentangle transcriptional and post-transcriptional regulatory processes, have revealed a more complex scenario. It is now possible to work out how synthesis, processing and degradation kinetic rates collectively determine the abundance of each gene’s RNA. It has become clear that the same transcriptional output can correspond to different combinations of the kinetic rates. This underscores the fact that markedly different modes of gene expression regulation exist, each with profound effects on a gene’s ability to modulate its own expression. This review describes the development of the experimental and computational approaches, including RNA metabolic labeling and mathematical modeling, that have been disclosing the mechanisms underlying complex transcriptional programs. Current limitations and future perspectives in the field are also discussed. |
format | Online Article Text |
id | pubmed-8294512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-82945122021-07-22 Dynamics of transcriptional and post-transcriptional regulation Furlan, Mattia de Pretis, Stefano Pelizzola, Mattia Brief Bioinform Method Review Despite gene expression programs being notoriously complex, RNA abundance is usually assumed as a proxy for transcriptional activity. Recently developed approaches, able to disentangle transcriptional and post-transcriptional regulatory processes, have revealed a more complex scenario. It is now possible to work out how synthesis, processing and degradation kinetic rates collectively determine the abundance of each gene’s RNA. It has become clear that the same transcriptional output can correspond to different combinations of the kinetic rates. This underscores the fact that markedly different modes of gene expression regulation exist, each with profound effects on a gene’s ability to modulate its own expression. This review describes the development of the experimental and computational approaches, including RNA metabolic labeling and mathematical modeling, that have been disclosing the mechanisms underlying complex transcriptional programs. Current limitations and future perspectives in the field are also discussed. Oxford University Press 2020-12-22 /pmc/articles/PMC8294512/ /pubmed/33348360 http://dx.doi.org/10.1093/bib/bbaa389 Text en © The Author(s) 2020. Published by Oxford University Press. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Method Review Furlan, Mattia de Pretis, Stefano Pelizzola, Mattia Dynamics of transcriptional and post-transcriptional regulation |
title | Dynamics of transcriptional and post-transcriptional regulation |
title_full | Dynamics of transcriptional and post-transcriptional regulation |
title_fullStr | Dynamics of transcriptional and post-transcriptional regulation |
title_full_unstemmed | Dynamics of transcriptional and post-transcriptional regulation |
title_short | Dynamics of transcriptional and post-transcriptional regulation |
title_sort | dynamics of transcriptional and post-transcriptional regulation |
topic | Method Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294512/ https://www.ncbi.nlm.nih.gov/pubmed/33348360 http://dx.doi.org/10.1093/bib/bbaa389 |
work_keys_str_mv | AT furlanmattia dynamicsoftranscriptionalandposttranscriptionalregulation AT depretisstefano dynamicsoftranscriptionalandposttranscriptionalregulation AT pelizzolamattia dynamicsoftranscriptionalandposttranscriptionalregulation |