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Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex
Subplate neurons (SPNs) are thought to play a role in nascent sensory processing in neocortex. To better understand how heterogeneity within this population relates to emergent function, we investigated the synaptic connectivity of Lpar1-EGFP SPNs through the first postnatal week in whisker somatose...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294844/ https://www.ncbi.nlm.nih.gov/pubmed/34251335 http://dx.doi.org/10.7554/eLife.60810 |
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author | Ghezzi, Filippo Marques-Smith, Andre Anastasiades, Paul G Lyngholm, Daniel Vagnoni, Cristiana Rowett, Alexandra Parameswaran, Gokul Hoerder-Suabedissen, Anna Nakagawa, Yasushi Molnar, Zoltan Butt, Simon JB |
author_facet | Ghezzi, Filippo Marques-Smith, Andre Anastasiades, Paul G Lyngholm, Daniel Vagnoni, Cristiana Rowett, Alexandra Parameswaran, Gokul Hoerder-Suabedissen, Anna Nakagawa, Yasushi Molnar, Zoltan Butt, Simon JB |
author_sort | Ghezzi, Filippo |
collection | PubMed |
description | Subplate neurons (SPNs) are thought to play a role in nascent sensory processing in neocortex. To better understand how heterogeneity within this population relates to emergent function, we investigated the synaptic connectivity of Lpar1-EGFP SPNs through the first postnatal week in whisker somatosensory cortex (S1BF). These SPNs comprise of two morphological subtypes: fusiform SPNs with local axons and pyramidal SPNs with axons that extend through the marginal zone. The former receive translaminar synaptic input up until the emergence of the whisker barrels, a timepoint coincident with significant cell death. In contrast, pyramidal SPNs receive local input from the subplate at early ages but then – during the later time window – acquire input from overlying cortex. Combined electrical and optogenetic activation of thalamic afferents identified that Lpar1-EGFP SPNs receive sparse thalamic innervation. These data reveal components of the postnatal network that interpret sparse thalamic input to direct the emergent columnar structure of S1BF. |
format | Online Article Text |
id | pubmed-8294844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-82948442021-07-23 Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex Ghezzi, Filippo Marques-Smith, Andre Anastasiades, Paul G Lyngholm, Daniel Vagnoni, Cristiana Rowett, Alexandra Parameswaran, Gokul Hoerder-Suabedissen, Anna Nakagawa, Yasushi Molnar, Zoltan Butt, Simon JB eLife Neuroscience Subplate neurons (SPNs) are thought to play a role in nascent sensory processing in neocortex. To better understand how heterogeneity within this population relates to emergent function, we investigated the synaptic connectivity of Lpar1-EGFP SPNs through the first postnatal week in whisker somatosensory cortex (S1BF). These SPNs comprise of two morphological subtypes: fusiform SPNs with local axons and pyramidal SPNs with axons that extend through the marginal zone. The former receive translaminar synaptic input up until the emergence of the whisker barrels, a timepoint coincident with significant cell death. In contrast, pyramidal SPNs receive local input from the subplate at early ages but then – during the later time window – acquire input from overlying cortex. Combined electrical and optogenetic activation of thalamic afferents identified that Lpar1-EGFP SPNs receive sparse thalamic innervation. These data reveal components of the postnatal network that interpret sparse thalamic input to direct the emergent columnar structure of S1BF. eLife Sciences Publications, Ltd 2021-07-12 /pmc/articles/PMC8294844/ /pubmed/34251335 http://dx.doi.org/10.7554/eLife.60810 Text en © 2021, Ghezzi et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Ghezzi, Filippo Marques-Smith, Andre Anastasiades, Paul G Lyngholm, Daniel Vagnoni, Cristiana Rowett, Alexandra Parameswaran, Gokul Hoerder-Suabedissen, Anna Nakagawa, Yasushi Molnar, Zoltan Butt, Simon JB Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex |
title | Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex |
title_full | Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex |
title_fullStr | Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex |
title_full_unstemmed | Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex |
title_short | Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex |
title_sort | non-canonical role for lpar1-egfp subplate neurons in early postnatal mouse somatosensory cortex |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294844/ https://www.ncbi.nlm.nih.gov/pubmed/34251335 http://dx.doi.org/10.7554/eLife.60810 |
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