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Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex

Subplate neurons (SPNs) are thought to play a role in nascent sensory processing in neocortex. To better understand how heterogeneity within this population relates to emergent function, we investigated the synaptic connectivity of Lpar1-EGFP SPNs through the first postnatal week in whisker somatose...

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Autores principales: Ghezzi, Filippo, Marques-Smith, Andre, Anastasiades, Paul G, Lyngholm, Daniel, Vagnoni, Cristiana, Rowett, Alexandra, Parameswaran, Gokul, Hoerder-Suabedissen, Anna, Nakagawa, Yasushi, Molnar, Zoltan, Butt, Simon JB
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294844/
https://www.ncbi.nlm.nih.gov/pubmed/34251335
http://dx.doi.org/10.7554/eLife.60810
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author Ghezzi, Filippo
Marques-Smith, Andre
Anastasiades, Paul G
Lyngholm, Daniel
Vagnoni, Cristiana
Rowett, Alexandra
Parameswaran, Gokul
Hoerder-Suabedissen, Anna
Nakagawa, Yasushi
Molnar, Zoltan
Butt, Simon JB
author_facet Ghezzi, Filippo
Marques-Smith, Andre
Anastasiades, Paul G
Lyngholm, Daniel
Vagnoni, Cristiana
Rowett, Alexandra
Parameswaran, Gokul
Hoerder-Suabedissen, Anna
Nakagawa, Yasushi
Molnar, Zoltan
Butt, Simon JB
author_sort Ghezzi, Filippo
collection PubMed
description Subplate neurons (SPNs) are thought to play a role in nascent sensory processing in neocortex. To better understand how heterogeneity within this population relates to emergent function, we investigated the synaptic connectivity of Lpar1-EGFP SPNs through the first postnatal week in whisker somatosensory cortex (S1BF). These SPNs comprise of two morphological subtypes: fusiform SPNs with local axons and pyramidal SPNs with axons that extend through the marginal zone. The former receive translaminar synaptic input up until the emergence of the whisker barrels, a timepoint coincident with significant cell death. In contrast, pyramidal SPNs receive local input from the subplate at early ages but then – during the later time window – acquire input from overlying cortex. Combined electrical and optogenetic activation of thalamic afferents identified that Lpar1-EGFP SPNs receive sparse thalamic innervation. These data reveal components of the postnatal network that interpret sparse thalamic input to direct the emergent columnar structure of S1BF.
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spelling pubmed-82948442021-07-23 Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex Ghezzi, Filippo Marques-Smith, Andre Anastasiades, Paul G Lyngholm, Daniel Vagnoni, Cristiana Rowett, Alexandra Parameswaran, Gokul Hoerder-Suabedissen, Anna Nakagawa, Yasushi Molnar, Zoltan Butt, Simon JB eLife Neuroscience Subplate neurons (SPNs) are thought to play a role in nascent sensory processing in neocortex. To better understand how heterogeneity within this population relates to emergent function, we investigated the synaptic connectivity of Lpar1-EGFP SPNs through the first postnatal week in whisker somatosensory cortex (S1BF). These SPNs comprise of two morphological subtypes: fusiform SPNs with local axons and pyramidal SPNs with axons that extend through the marginal zone. The former receive translaminar synaptic input up until the emergence of the whisker barrels, a timepoint coincident with significant cell death. In contrast, pyramidal SPNs receive local input from the subplate at early ages but then – during the later time window – acquire input from overlying cortex. Combined electrical and optogenetic activation of thalamic afferents identified that Lpar1-EGFP SPNs receive sparse thalamic innervation. These data reveal components of the postnatal network that interpret sparse thalamic input to direct the emergent columnar structure of S1BF. eLife Sciences Publications, Ltd 2021-07-12 /pmc/articles/PMC8294844/ /pubmed/34251335 http://dx.doi.org/10.7554/eLife.60810 Text en © 2021, Ghezzi et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Ghezzi, Filippo
Marques-Smith, Andre
Anastasiades, Paul G
Lyngholm, Daniel
Vagnoni, Cristiana
Rowett, Alexandra
Parameswaran, Gokul
Hoerder-Suabedissen, Anna
Nakagawa, Yasushi
Molnar, Zoltan
Butt, Simon JB
Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex
title Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex
title_full Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex
title_fullStr Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex
title_full_unstemmed Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex
title_short Non-canonical role for Lpar1-EGFP subplate neurons in early postnatal mouse somatosensory cortex
title_sort non-canonical role for lpar1-egfp subplate neurons in early postnatal mouse somatosensory cortex
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294844/
https://www.ncbi.nlm.nih.gov/pubmed/34251335
http://dx.doi.org/10.7554/eLife.60810
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