Evaluation of two rapid phenotypical tests—Alifax rapid AST colistin test and Rapid Polymyxin NP test—for detection of colistin resistance in Enterobacterales

Our study evaluates the performance of two rapid phenotypical tests to detect colistin resistance in Enterobacterales: Alifax rapid AST colistin test using the HB&L system and Rapid Polymyxin NP test prepared in-house. A collection of well-characterized 53 colistin-susceptible and 66 colistin-re...

Descripción completa

Detalles Bibliográficos
Autores principales: Germ, Julija, Seme, Katja, Cerar, Tjasa, Krizan Hergouth, Veronika, Pirs, Mateja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295142/
https://www.ncbi.nlm.nih.gov/pubmed/33595758
http://dx.doi.org/10.1007/s10096-021-04182-w
_version_ 1783725380811620352
author Germ, Julija
Seme, Katja
Cerar, Tjasa
Krizan Hergouth, Veronika
Pirs, Mateja
author_facet Germ, Julija
Seme, Katja
Cerar, Tjasa
Krizan Hergouth, Veronika
Pirs, Mateja
author_sort Germ, Julija
collection PubMed
description Our study evaluates the performance of two rapid phenotypical tests to detect colistin resistance in Enterobacterales: Alifax rapid AST colistin test using the HB&L system and Rapid Polymyxin NP test prepared in-house. A collection of well-characterized 53 colistin-susceptible and 66 colistin-resistantEnterobacterales isolates was used. The results obtained using both rapid tests were compared to the reference broth microdilution. Overall categorical agreement was 81.5% for Alifax test and 98.3% for Rapid Polymyxin NP test. Based on our results, the Rapid Polymyxin NP test is superior to the Alifax test that performed inadequate for Enterobacter spp. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10096-021-04182-w.
format Online
Article
Text
id pubmed-8295142
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-82951422021-07-23 Evaluation of two rapid phenotypical tests—Alifax rapid AST colistin test and Rapid Polymyxin NP test—for detection of colistin resistance in Enterobacterales Germ, Julija Seme, Katja Cerar, Tjasa Krizan Hergouth, Veronika Pirs, Mateja Eur J Clin Microbiol Infect Dis Brief Report Our study evaluates the performance of two rapid phenotypical tests to detect colistin resistance in Enterobacterales: Alifax rapid AST colistin test using the HB&L system and Rapid Polymyxin NP test prepared in-house. A collection of well-characterized 53 colistin-susceptible and 66 colistin-resistantEnterobacterales isolates was used. The results obtained using both rapid tests were compared to the reference broth microdilution. Overall categorical agreement was 81.5% for Alifax test and 98.3% for Rapid Polymyxin NP test. Based on our results, the Rapid Polymyxin NP test is superior to the Alifax test that performed inadequate for Enterobacter spp. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10096-021-04182-w. Springer Berlin Heidelberg 2021-02-17 2021 /pmc/articles/PMC8295142/ /pubmed/33595758 http://dx.doi.org/10.1007/s10096-021-04182-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Report
Germ, Julija
Seme, Katja
Cerar, Tjasa
Krizan Hergouth, Veronika
Pirs, Mateja
Evaluation of two rapid phenotypical tests—Alifax rapid AST colistin test and Rapid Polymyxin NP test—for detection of colistin resistance in Enterobacterales
title Evaluation of two rapid phenotypical tests—Alifax rapid AST colistin test and Rapid Polymyxin NP test—for detection of colistin resistance in Enterobacterales
title_full Evaluation of two rapid phenotypical tests—Alifax rapid AST colistin test and Rapid Polymyxin NP test—for detection of colistin resistance in Enterobacterales
title_fullStr Evaluation of two rapid phenotypical tests—Alifax rapid AST colistin test and Rapid Polymyxin NP test—for detection of colistin resistance in Enterobacterales
title_full_unstemmed Evaluation of two rapid phenotypical tests—Alifax rapid AST colistin test and Rapid Polymyxin NP test—for detection of colistin resistance in Enterobacterales
title_short Evaluation of two rapid phenotypical tests—Alifax rapid AST colistin test and Rapid Polymyxin NP test—for detection of colistin resistance in Enterobacterales
title_sort evaluation of two rapid phenotypical tests—alifax rapid ast colistin test and rapid polymyxin np test—for detection of colistin resistance in enterobacterales
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295142/
https://www.ncbi.nlm.nih.gov/pubmed/33595758
http://dx.doi.org/10.1007/s10096-021-04182-w
work_keys_str_mv AT germjulija evaluationoftworapidphenotypicaltestsalifaxrapidastcolistintestandrapidpolymyxinnptestfordetectionofcolistinresistanceinenterobacterales
AT semekatja evaluationoftworapidphenotypicaltestsalifaxrapidastcolistintestandrapidpolymyxinnptestfordetectionofcolistinresistanceinenterobacterales
AT cerartjasa evaluationoftworapidphenotypicaltestsalifaxrapidastcolistintestandrapidpolymyxinnptestfordetectionofcolistinresistanceinenterobacterales
AT krizanhergouthveronika evaluationoftworapidphenotypicaltestsalifaxrapidastcolistintestandrapidpolymyxinnptestfordetectionofcolistinresistanceinenterobacterales
AT pirsmateja evaluationoftworapidphenotypicaltestsalifaxrapidastcolistintestandrapidpolymyxinnptestfordetectionofcolistinresistanceinenterobacterales

Ejemplares similares