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Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2
The impact of repeated sample collection on COVID-19 test performance is unknown. The FDA and CDC currently recommend the primary collection of diagnostic samples to minimize the perceived risk of false-negative findings. We therefore evaluated the association between repeated sample collection and...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295385/ https://www.ncbi.nlm.nih.gov/pubmed/34290378 http://dx.doi.org/10.1038/s41598-021-94547-0 |
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| author | Levy, Joshua M. Frediani, Jennifer K. Tyburski, Erika A. Wood, Anna Figueroa, Janet Kempker, Russell R. Rebolledo, Paulina A. Gonzalez, Mark D. Sullivan, Julie Vos, Miriam B. O’Neal, Jared Martin, Greg S. Lam, Wilbur A. Waggoner, Jesse J. |
| author_facet | Levy, Joshua M. Frediani, Jennifer K. Tyburski, Erika A. Wood, Anna Figueroa, Janet Kempker, Russell R. Rebolledo, Paulina A. Gonzalez, Mark D. Sullivan, Julie Vos, Miriam B. O’Neal, Jared Martin, Greg S. Lam, Wilbur A. Waggoner, Jesse J. |
| author_sort | Levy, Joshua M. |
| collection | PubMed |
| description | The impact of repeated sample collection on COVID-19 test performance is unknown. The FDA and CDC currently recommend the primary collection of diagnostic samples to minimize the perceived risk of false-negative findings. We therefore evaluated the association between repeated sample collection and test performance among 325 symptomatic patients undergoing COVID-19 testing in Atlanta, GA. High concordance was found between consecutively collected mid-turbinate samples with both molecular (n = 74, 100% concordance) and antigen-based (n = 147, 97% concordance, kappa = 0.95, CI = 0.88–1.00) diagnostic assays. Repeated sample collection does not decrease COVID-19 test performance, demonstrating that multiple samples can be collected for assay validation and clinical diagnosis. |
| format | Online Article Text |
| id | pubmed-8295385 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-82953852021-07-23 Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 Levy, Joshua M. Frediani, Jennifer K. Tyburski, Erika A. Wood, Anna Figueroa, Janet Kempker, Russell R. Rebolledo, Paulina A. Gonzalez, Mark D. Sullivan, Julie Vos, Miriam B. O’Neal, Jared Martin, Greg S. Lam, Wilbur A. Waggoner, Jesse J. Sci Rep Article The impact of repeated sample collection on COVID-19 test performance is unknown. The FDA and CDC currently recommend the primary collection of diagnostic samples to minimize the perceived risk of false-negative findings. We therefore evaluated the association between repeated sample collection and test performance among 325 symptomatic patients undergoing COVID-19 testing in Atlanta, GA. High concordance was found between consecutively collected mid-turbinate samples with both molecular (n = 74, 100% concordance) and antigen-based (n = 147, 97% concordance, kappa = 0.95, CI = 0.88–1.00) diagnostic assays. Repeated sample collection does not decrease COVID-19 test performance, demonstrating that multiple samples can be collected for assay validation and clinical diagnosis. Nature Publishing Group UK 2021-07-21 /pmc/articles/PMC8295385/ /pubmed/34290378 http://dx.doi.org/10.1038/s41598-021-94547-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Article Levy, Joshua M. Frediani, Jennifer K. Tyburski, Erika A. Wood, Anna Figueroa, Janet Kempker, Russell R. Rebolledo, Paulina A. Gonzalez, Mark D. Sullivan, Julie Vos, Miriam B. O’Neal, Jared Martin, Greg S. Lam, Wilbur A. Waggoner, Jesse J. Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 |
| title | Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 |
| title_full | Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 |
| title_fullStr | Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 |
| title_full_unstemmed | Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 |
| title_short | Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2 |
| title_sort | impact of repeated nasal sampling on detection and quantification of sars-cov-2 |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295385/ https://www.ncbi.nlm.nih.gov/pubmed/34290378 http://dx.doi.org/10.1038/s41598-021-94547-0 |
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