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Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine

Apicomplexan parasites, including Toxoplasma gondii (T. gondii), express different types of calcium-dependent protein kinases (CDPKs), which perform a variety of functions, including attacking and exiting the host cells. In the current bioinformatics study, we have used several web servers to predic...

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Autores principales: Taghipour, Ali, Tavakoli, Sanaz, Sabaghan, Mohamad, Foroutan, Masoud, Majidiani, Hamidreza, Soltani, Shahrzad, Badri, Milad, Ghaffari, Ali Dalir, Soltani, Sheyda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295510/
https://www.ncbi.nlm.nih.gov/pubmed/34336254
http://dx.doi.org/10.1155/2021/9974509
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author Taghipour, Ali
Tavakoli, Sanaz
Sabaghan, Mohamad
Foroutan, Masoud
Majidiani, Hamidreza
Soltani, Shahrzad
Badri, Milad
Ghaffari, Ali Dalir
Soltani, Sheyda
author_facet Taghipour, Ali
Tavakoli, Sanaz
Sabaghan, Mohamad
Foroutan, Masoud
Majidiani, Hamidreza
Soltani, Shahrzad
Badri, Milad
Ghaffari, Ali Dalir
Soltani, Sheyda
author_sort Taghipour, Ali
collection PubMed
description Apicomplexan parasites, including Toxoplasma gondii (T. gondii), express different types of calcium-dependent protein kinases (CDPKs), which perform a variety of functions, including attacking and exiting the host cells. In the current bioinformatics study, we have used several web servers to predict the basic features and specifications of the CDPK7 protein. The findings showed that CDPK7 protein has 2133 amino acid residues with an average molecular weight (MW) of 219085.79 D. The aliphatic index with 68.78 and grand average of hydropathicity (GRAVY) with -0.331 score were estimated. The outcomes of current research showed that the CDPK7 protein included 502 alpha-helix, 1311 random coils, and 320 extended strands with GOR4 method. Considering the Ramachandran plot, the favored region contains more than 92% of the amino acid residues. In addition, evaluation of antigenicity and allergenicity showed that CDPK7 protein has immunogenic and nonallergenic nature. The present research provides key data for more animal-model study on the CDPK7 protein to design an efficient vaccine against toxoplasmosis in the future.
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spelling pubmed-82955102021-07-31 Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine Taghipour, Ali Tavakoli, Sanaz Sabaghan, Mohamad Foroutan, Masoud Majidiani, Hamidreza Soltani, Shahrzad Badri, Milad Ghaffari, Ali Dalir Soltani, Sheyda J Parasitol Res Research Article Apicomplexan parasites, including Toxoplasma gondii (T. gondii), express different types of calcium-dependent protein kinases (CDPKs), which perform a variety of functions, including attacking and exiting the host cells. In the current bioinformatics study, we have used several web servers to predict the basic features and specifications of the CDPK7 protein. The findings showed that CDPK7 protein has 2133 amino acid residues with an average molecular weight (MW) of 219085.79 D. The aliphatic index with 68.78 and grand average of hydropathicity (GRAVY) with -0.331 score were estimated. The outcomes of current research showed that the CDPK7 protein included 502 alpha-helix, 1311 random coils, and 320 extended strands with GOR4 method. Considering the Ramachandran plot, the favored region contains more than 92% of the amino acid residues. In addition, evaluation of antigenicity and allergenicity showed that CDPK7 protein has immunogenic and nonallergenic nature. The present research provides key data for more animal-model study on the CDPK7 protein to design an efficient vaccine against toxoplasmosis in the future. Hindawi 2021-07-08 /pmc/articles/PMC8295510/ /pubmed/34336254 http://dx.doi.org/10.1155/2021/9974509 Text en Copyright © 2021 Ali Taghipour et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Taghipour, Ali
Tavakoli, Sanaz
Sabaghan, Mohamad
Foroutan, Masoud
Majidiani, Hamidreza
Soltani, Shahrzad
Badri, Milad
Ghaffari, Ali Dalir
Soltani, Sheyda
Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine
title Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine
title_full Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine
title_fullStr Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine
title_full_unstemmed Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine
title_short Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine
title_sort immunoinformatic analysis of calcium-dependent protein kinase 7 (cdpk7) showed potential targets for toxoplasma gondii vaccine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295510/
https://www.ncbi.nlm.nih.gov/pubmed/34336254
http://dx.doi.org/10.1155/2021/9974509
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