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A 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum

Amphidiploid fungal Verticillium longisporum strains Vl43 and Vl32 colonize the plant host Brassica napus but differ in their ability to cause disease symptoms. These strains represent two V. longisporum lineages derived from different hybridization events of haploid parental Verticillium strains. V...

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Autores principales: Harting, Rebekka, Starke, Jessica, Kusch, Harald, Pöggeler, Stefanie, Maurus, Isabel, Schlüter, Rabea, Landesfeind, Manuel, Bulla, Ingo, Nowrousian, Minou, de Jonge, Ronnie, Stahlhut, Gertrud, Hoff, Katharina J., Aßhauer, Kathrin P., Thürmer, Andrea, Stanke, Mario, Daniel, Rolf, Morgenstern, Burkhard, Thomma, Bart P. H. J., Kronstad, James W., Braus‐Stromeyer, Susanna A., Braus, Gerhard H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295516/
https://www.ncbi.nlm.nih.gov/pubmed/33955130
http://dx.doi.org/10.1111/mpp.13071
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author Harting, Rebekka
Starke, Jessica
Kusch, Harald
Pöggeler, Stefanie
Maurus, Isabel
Schlüter, Rabea
Landesfeind, Manuel
Bulla, Ingo
Nowrousian, Minou
de Jonge, Ronnie
Stahlhut, Gertrud
Hoff, Katharina J.
Aßhauer, Kathrin P.
Thürmer, Andrea
Stanke, Mario
Daniel, Rolf
Morgenstern, Burkhard
Thomma, Bart P. H. J.
Kronstad, James W.
Braus‐Stromeyer, Susanna A.
Braus, Gerhard H.
author_facet Harting, Rebekka
Starke, Jessica
Kusch, Harald
Pöggeler, Stefanie
Maurus, Isabel
Schlüter, Rabea
Landesfeind, Manuel
Bulla, Ingo
Nowrousian, Minou
de Jonge, Ronnie
Stahlhut, Gertrud
Hoff, Katharina J.
Aßhauer, Kathrin P.
Thürmer, Andrea
Stanke, Mario
Daniel, Rolf
Morgenstern, Burkhard
Thomma, Bart P. H. J.
Kronstad, James W.
Braus‐Stromeyer, Susanna A.
Braus, Gerhard H.
author_sort Harting, Rebekka
collection PubMed
description Amphidiploid fungal Verticillium longisporum strains Vl43 and Vl32 colonize the plant host Brassica napus but differ in their ability to cause disease symptoms. These strains represent two V. longisporum lineages derived from different hybridization events of haploid parental Verticillium strains. Vl32 and Vl43 carry same‐sex mating‐type genes derived from both parental lineages. Vl32 and Vl43 similarly colonize and penetrate plant roots, but asymptomatic Vl32 proliferation in planta is lower than virulent Vl43. The highly conserved Vl43 and Vl32 genomes include less than 1% unique genes, and the karyotypes of 15 or 16 chromosomes display changed genetic synteny due to substantial genomic reshuffling. A 20 kb Vl43 lineage‐specific (LS) region apparently originating from the Verticillium dahliae‐related ancestor is specific for symptomatic Vl43 and encodes seven genes, including two putative transcription factors. Either partial or complete deletion of this LS region in Vl43 did not reduce virulence but led to induction of even more severe disease symptoms in rapeseed. This suggests that the LS insertion in the genome of symptomatic V. longisporum Vl43 mediates virulence‐reducing functions, limits damage on the host plant, and therefore tames Vl43 from being even more virulent.
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spelling pubmed-82955162021-07-27 A 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum Harting, Rebekka Starke, Jessica Kusch, Harald Pöggeler, Stefanie Maurus, Isabel Schlüter, Rabea Landesfeind, Manuel Bulla, Ingo Nowrousian, Minou de Jonge, Ronnie Stahlhut, Gertrud Hoff, Katharina J. Aßhauer, Kathrin P. Thürmer, Andrea Stanke, Mario Daniel, Rolf Morgenstern, Burkhard Thomma, Bart P. H. J. Kronstad, James W. Braus‐Stromeyer, Susanna A. Braus, Gerhard H. Mol Plant Pathol Original Articles Amphidiploid fungal Verticillium longisporum strains Vl43 and Vl32 colonize the plant host Brassica napus but differ in their ability to cause disease symptoms. These strains represent two V. longisporum lineages derived from different hybridization events of haploid parental Verticillium strains. Vl32 and Vl43 carry same‐sex mating‐type genes derived from both parental lineages. Vl32 and Vl43 similarly colonize and penetrate plant roots, but asymptomatic Vl32 proliferation in planta is lower than virulent Vl43. The highly conserved Vl43 and Vl32 genomes include less than 1% unique genes, and the karyotypes of 15 or 16 chromosomes display changed genetic synteny due to substantial genomic reshuffling. A 20 kb Vl43 lineage‐specific (LS) region apparently originating from the Verticillium dahliae‐related ancestor is specific for symptomatic Vl43 and encodes seven genes, including two putative transcription factors. Either partial or complete deletion of this LS region in Vl43 did not reduce virulence but led to induction of even more severe disease symptoms in rapeseed. This suggests that the LS insertion in the genome of symptomatic V. longisporum Vl43 mediates virulence‐reducing functions, limits damage on the host plant, and therefore tames Vl43 from being even more virulent. John Wiley and Sons Inc. 2021-05-05 /pmc/articles/PMC8295516/ /pubmed/33955130 http://dx.doi.org/10.1111/mpp.13071 Text en © 2021 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Harting, Rebekka
Starke, Jessica
Kusch, Harald
Pöggeler, Stefanie
Maurus, Isabel
Schlüter, Rabea
Landesfeind, Manuel
Bulla, Ingo
Nowrousian, Minou
de Jonge, Ronnie
Stahlhut, Gertrud
Hoff, Katharina J.
Aßhauer, Kathrin P.
Thürmer, Andrea
Stanke, Mario
Daniel, Rolf
Morgenstern, Burkhard
Thomma, Bart P. H. J.
Kronstad, James W.
Braus‐Stromeyer, Susanna A.
Braus, Gerhard H.
A 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum
title A 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum
title_full A 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum
title_fullStr A 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum
title_full_unstemmed A 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum
title_short A 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum
title_sort 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid verticillium longisporum
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295516/
https://www.ncbi.nlm.nih.gov/pubmed/33955130
http://dx.doi.org/10.1111/mpp.13071
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