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Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses

BACKGROUND: Sphingolipids modulate insulin sensitivity in mammals. Increased synthesis of ceramides is linked to decreased insulin sensitivity of tissues. Conversely, activation of the insulin signaling pathway can downregulate ceramide synthesis. Elucidating the association between sphingolipid met...

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Autores principales: Leung, Yue Hei, Kenéz, Ákos, Grob, Anne Julia, Feige, Karsten, Warnken, Tobias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295691/
https://www.ncbi.nlm.nih.gov/pubmed/34105193
http://dx.doi.org/10.1111/jvim.16200
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author Leung, Yue Hei
Kenéz, Ákos
Grob, Anne Julia
Feige, Karsten
Warnken, Tobias
author_facet Leung, Yue Hei
Kenéz, Ákos
Grob, Anne Julia
Feige, Karsten
Warnken, Tobias
author_sort Leung, Yue Hei
collection PubMed
description BACKGROUND: Sphingolipids modulate insulin sensitivity in mammals. Increased synthesis of ceramides is linked to decreased insulin sensitivity of tissues. Conversely, activation of the insulin signaling pathway can downregulate ceramide synthesis. Elucidating the association between sphingolipid metabolism and insulin response during oral glucose testing may help explain the pathophysiology of insulin dysregulation in horses. HYPOTHESES: Horses with insulin dysregulation will have a plasma sphingolipid profile characterized by increased ceramide concentrations. The plasma sphingolipid profile will have decreased ceramide concentrations after acute activation of the insulin signaling pathway by oral glucose testing. ANIMALS: Twelve Icelandic horses. METHODS: Horses were subjected to an oral glucose test (0.5 g/kg body weight glucose), with plasma insulin concentrations measured at 0, 30, 60, 120, 180, and 240 minutes postglucose administration. Plasma samples were collected at 0 and 120 minutes for sphingolipid profiling using a liquid chromatography‐mass spectrometry‐based metabolomics analysis. Eighty‐three species of sphingolipids were detected, including 3‐ketosphinganines, dihydroceramides, ceramides, dihydrosphingomyelins, sphingomyelins, galatosylceramides, glucosylceramides, lactosylceramides, and ceramide‐1‐phosphates. RESULTS: Glucose administration did not significantly alter plasma sphingolipid profiles. C22:0‐ceramide, C24:1‐ceramide, C23:0‐ceramide, C16:1‐sphingomyelin, C22:0‐dihydroceramide, and C24:0‐ceramide were positively correlated with the insulin response (area under the curve). CONCLUSION AND CLINICAL IMPORTANCE: Positive correlation between the insulin response and sphingolipid concentrations implies upregulated sphingolipid metabolism in insulin dysregulated horses. A high plasma ceramide concentration can indicate insulin dysregulation in horses.
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spelling pubmed-82956912021-07-27 Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses Leung, Yue Hei Kenéz, Ákos Grob, Anne Julia Feige, Karsten Warnken, Tobias J Vet Intern Med EQUINE BACKGROUND: Sphingolipids modulate insulin sensitivity in mammals. Increased synthesis of ceramides is linked to decreased insulin sensitivity of tissues. Conversely, activation of the insulin signaling pathway can downregulate ceramide synthesis. Elucidating the association between sphingolipid metabolism and insulin response during oral glucose testing may help explain the pathophysiology of insulin dysregulation in horses. HYPOTHESES: Horses with insulin dysregulation will have a plasma sphingolipid profile characterized by increased ceramide concentrations. The plasma sphingolipid profile will have decreased ceramide concentrations after acute activation of the insulin signaling pathway by oral glucose testing. ANIMALS: Twelve Icelandic horses. METHODS: Horses were subjected to an oral glucose test (0.5 g/kg body weight glucose), with plasma insulin concentrations measured at 0, 30, 60, 120, 180, and 240 minutes postglucose administration. Plasma samples were collected at 0 and 120 minutes for sphingolipid profiling using a liquid chromatography‐mass spectrometry‐based metabolomics analysis. Eighty‐three species of sphingolipids were detected, including 3‐ketosphinganines, dihydroceramides, ceramides, dihydrosphingomyelins, sphingomyelins, galatosylceramides, glucosylceramides, lactosylceramides, and ceramide‐1‐phosphates. RESULTS: Glucose administration did not significantly alter plasma sphingolipid profiles. C22:0‐ceramide, C24:1‐ceramide, C23:0‐ceramide, C16:1‐sphingomyelin, C22:0‐dihydroceramide, and C24:0‐ceramide were positively correlated with the insulin response (area under the curve). CONCLUSION AND CLINICAL IMPORTANCE: Positive correlation between the insulin response and sphingolipid concentrations implies upregulated sphingolipid metabolism in insulin dysregulated horses. A high plasma ceramide concentration can indicate insulin dysregulation in horses. John Wiley & Sons, Inc. 2021-06-08 2021 /pmc/articles/PMC8295691/ /pubmed/34105193 http://dx.doi.org/10.1111/jvim.16200 Text en © 2021 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle EQUINE
Leung, Yue Hei
Kenéz, Ákos
Grob, Anne Julia
Feige, Karsten
Warnken, Tobias
Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses
title Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses
title_full Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses
title_fullStr Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses
title_full_unstemmed Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses
title_short Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses
title_sort associations of plasma sphingolipid profiles with insulin response during oral glucose testing in icelandic horses
topic EQUINE
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295691/
https://www.ncbi.nlm.nih.gov/pubmed/34105193
http://dx.doi.org/10.1111/jvim.16200
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