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Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses
BACKGROUND: Sphingolipids modulate insulin sensitivity in mammals. Increased synthesis of ceramides is linked to decreased insulin sensitivity of tissues. Conversely, activation of the insulin signaling pathway can downregulate ceramide synthesis. Elucidating the association between sphingolipid met...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295691/ https://www.ncbi.nlm.nih.gov/pubmed/34105193 http://dx.doi.org/10.1111/jvim.16200 |
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author | Leung, Yue Hei Kenéz, Ákos Grob, Anne Julia Feige, Karsten Warnken, Tobias |
author_facet | Leung, Yue Hei Kenéz, Ákos Grob, Anne Julia Feige, Karsten Warnken, Tobias |
author_sort | Leung, Yue Hei |
collection | PubMed |
description | BACKGROUND: Sphingolipids modulate insulin sensitivity in mammals. Increased synthesis of ceramides is linked to decreased insulin sensitivity of tissues. Conversely, activation of the insulin signaling pathway can downregulate ceramide synthesis. Elucidating the association between sphingolipid metabolism and insulin response during oral glucose testing may help explain the pathophysiology of insulin dysregulation in horses. HYPOTHESES: Horses with insulin dysregulation will have a plasma sphingolipid profile characterized by increased ceramide concentrations. The plasma sphingolipid profile will have decreased ceramide concentrations after acute activation of the insulin signaling pathway by oral glucose testing. ANIMALS: Twelve Icelandic horses. METHODS: Horses were subjected to an oral glucose test (0.5 g/kg body weight glucose), with plasma insulin concentrations measured at 0, 30, 60, 120, 180, and 240 minutes postglucose administration. Plasma samples were collected at 0 and 120 minutes for sphingolipid profiling using a liquid chromatography‐mass spectrometry‐based metabolomics analysis. Eighty‐three species of sphingolipids were detected, including 3‐ketosphinganines, dihydroceramides, ceramides, dihydrosphingomyelins, sphingomyelins, galatosylceramides, glucosylceramides, lactosylceramides, and ceramide‐1‐phosphates. RESULTS: Glucose administration did not significantly alter plasma sphingolipid profiles. C22:0‐ceramide, C24:1‐ceramide, C23:0‐ceramide, C16:1‐sphingomyelin, C22:0‐dihydroceramide, and C24:0‐ceramide were positively correlated with the insulin response (area under the curve). CONCLUSION AND CLINICAL IMPORTANCE: Positive correlation between the insulin response and sphingolipid concentrations implies upregulated sphingolipid metabolism in insulin dysregulated horses. A high plasma ceramide concentration can indicate insulin dysregulation in horses. |
format | Online Article Text |
id | pubmed-8295691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82956912021-07-27 Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses Leung, Yue Hei Kenéz, Ákos Grob, Anne Julia Feige, Karsten Warnken, Tobias J Vet Intern Med EQUINE BACKGROUND: Sphingolipids modulate insulin sensitivity in mammals. Increased synthesis of ceramides is linked to decreased insulin sensitivity of tissues. Conversely, activation of the insulin signaling pathway can downregulate ceramide synthesis. Elucidating the association between sphingolipid metabolism and insulin response during oral glucose testing may help explain the pathophysiology of insulin dysregulation in horses. HYPOTHESES: Horses with insulin dysregulation will have a plasma sphingolipid profile characterized by increased ceramide concentrations. The plasma sphingolipid profile will have decreased ceramide concentrations after acute activation of the insulin signaling pathway by oral glucose testing. ANIMALS: Twelve Icelandic horses. METHODS: Horses were subjected to an oral glucose test (0.5 g/kg body weight glucose), with plasma insulin concentrations measured at 0, 30, 60, 120, 180, and 240 minutes postglucose administration. Plasma samples were collected at 0 and 120 minutes for sphingolipid profiling using a liquid chromatography‐mass spectrometry‐based metabolomics analysis. Eighty‐three species of sphingolipids were detected, including 3‐ketosphinganines, dihydroceramides, ceramides, dihydrosphingomyelins, sphingomyelins, galatosylceramides, glucosylceramides, lactosylceramides, and ceramide‐1‐phosphates. RESULTS: Glucose administration did not significantly alter plasma sphingolipid profiles. C22:0‐ceramide, C24:1‐ceramide, C23:0‐ceramide, C16:1‐sphingomyelin, C22:0‐dihydroceramide, and C24:0‐ceramide were positively correlated with the insulin response (area under the curve). CONCLUSION AND CLINICAL IMPORTANCE: Positive correlation between the insulin response and sphingolipid concentrations implies upregulated sphingolipid metabolism in insulin dysregulated horses. A high plasma ceramide concentration can indicate insulin dysregulation in horses. John Wiley & Sons, Inc. 2021-06-08 2021 /pmc/articles/PMC8295691/ /pubmed/34105193 http://dx.doi.org/10.1111/jvim.16200 Text en © 2021 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | EQUINE Leung, Yue Hei Kenéz, Ákos Grob, Anne Julia Feige, Karsten Warnken, Tobias Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses |
title | Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses |
title_full | Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses |
title_fullStr | Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses |
title_full_unstemmed | Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses |
title_short | Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses |
title_sort | associations of plasma sphingolipid profiles with insulin response during oral glucose testing in icelandic horses |
topic | EQUINE |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295691/ https://www.ncbi.nlm.nih.gov/pubmed/34105193 http://dx.doi.org/10.1111/jvim.16200 |
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