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Presumptive Identification of Smooth Brucella Strain Antibodies in Canines

Brucellosis is a zoonotic disease caused by a Gram-negative coccobacillus. There are four Brucella strains of zoonotic importance in our domestic species, subdivided by their culture phenotypes: Brucella abortus (B. abortus), B. melitensis, B. suis (smooth strains) and B. canis (rough strain). Dogs...

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Autores principales: Helms, Alyssa B., Balogh, Orsolya, Franklin-Guild, Rebecca, Lahmers, Kevin, Caswell, Clayton C., Cecere, Julie T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295921/
https://www.ncbi.nlm.nih.gov/pubmed/34307536
http://dx.doi.org/10.3389/fvets.2021.697479
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author Helms, Alyssa B.
Balogh, Orsolya
Franklin-Guild, Rebecca
Lahmers, Kevin
Caswell, Clayton C.
Cecere, Julie T.
author_facet Helms, Alyssa B.
Balogh, Orsolya
Franklin-Guild, Rebecca
Lahmers, Kevin
Caswell, Clayton C.
Cecere, Julie T.
author_sort Helms, Alyssa B.
collection PubMed
description Brucellosis is a zoonotic disease caused by a Gram-negative coccobacillus. There are four Brucella strains of zoonotic importance in our domestic species, subdivided by their culture phenotypes: Brucella abortus (B. abortus), B. melitensis, B. suis (smooth strains) and B. canis (rough strain). Dogs can serve as hosts for all four of the zoonotic strains; however, routine serologic testing in dogs has been limited to the identification of B. canis antibodies. The aim of our study was to identify smooth Brucella strain antibodies in canines. We hypothesize that the Brucella abortus Fluorescence Polarization Assay would be successful in identifying smooth Brucella strain antibodies in canines. Ninety-five dogs, including forty-five hog hunting dogs were screened for circulating antibodies to any of the four zoonotic strains of the bacteria utilizing a combination of Canine Brucella Slide Agglutination Test (CBSA), Brucella canis Agar Gel Immunodiffusion II test (AGIDII), Brucella abortus Card Agglutination Test (BCA), and the Brucella abortus Fluorescence Polarization Assay (FPA). Test interpretation results yielded a 0% (0/95) smooth Brucella strain seropositivity rate, with 2% (2/95) of dogs yielding inconclusive rough Brucella strain serology results (0–2% rough strain seropositivity rate). Additionally, a retrospective portion of the study was performed to identify sera containing circulating antibodies to any of the smooth strains of Brucella by testing previously banked canine serum samples stored at Cornell's Veterinary Diagnostic Laboratory from 2018 to 2019 via Brucella abortus FPA. Of the 769 serum samples tested, 13/769 (1.7%) yielded an inconclusive result, 725/769 (94.2%) were negative, 30/769 (4%) yielded a positive FPA test result, and 1/769 (0.1%) had to be excluded due to insufficient sample remaining to perform the diagnostic test. Of the 30 FPA positive canine serum samples, 97% (29/30) also tested positive on the CBSA test. Additionally, there was a statistically significant (p < 0.0001) likelihood of altered (spayed/neutered) and mixed breed dogs to be FPA positive when compared to intact, purebred dogs, respectively.
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spelling pubmed-82959212021-07-23 Presumptive Identification of Smooth Brucella Strain Antibodies in Canines Helms, Alyssa B. Balogh, Orsolya Franklin-Guild, Rebecca Lahmers, Kevin Caswell, Clayton C. Cecere, Julie T. Front Vet Sci Veterinary Science Brucellosis is a zoonotic disease caused by a Gram-negative coccobacillus. There are four Brucella strains of zoonotic importance in our domestic species, subdivided by their culture phenotypes: Brucella abortus (B. abortus), B. melitensis, B. suis (smooth strains) and B. canis (rough strain). Dogs can serve as hosts for all four of the zoonotic strains; however, routine serologic testing in dogs has been limited to the identification of B. canis antibodies. The aim of our study was to identify smooth Brucella strain antibodies in canines. We hypothesize that the Brucella abortus Fluorescence Polarization Assay would be successful in identifying smooth Brucella strain antibodies in canines. Ninety-five dogs, including forty-five hog hunting dogs were screened for circulating antibodies to any of the four zoonotic strains of the bacteria utilizing a combination of Canine Brucella Slide Agglutination Test (CBSA), Brucella canis Agar Gel Immunodiffusion II test (AGIDII), Brucella abortus Card Agglutination Test (BCA), and the Brucella abortus Fluorescence Polarization Assay (FPA). Test interpretation results yielded a 0% (0/95) smooth Brucella strain seropositivity rate, with 2% (2/95) of dogs yielding inconclusive rough Brucella strain serology results (0–2% rough strain seropositivity rate). Additionally, a retrospective portion of the study was performed to identify sera containing circulating antibodies to any of the smooth strains of Brucella by testing previously banked canine serum samples stored at Cornell's Veterinary Diagnostic Laboratory from 2018 to 2019 via Brucella abortus FPA. Of the 769 serum samples tested, 13/769 (1.7%) yielded an inconclusive result, 725/769 (94.2%) were negative, 30/769 (4%) yielded a positive FPA test result, and 1/769 (0.1%) had to be excluded due to insufficient sample remaining to perform the diagnostic test. Of the 30 FPA positive canine serum samples, 97% (29/30) also tested positive on the CBSA test. Additionally, there was a statistically significant (p < 0.0001) likelihood of altered (spayed/neutered) and mixed breed dogs to be FPA positive when compared to intact, purebred dogs, respectively. Frontiers Media S.A. 2021-07-08 /pmc/articles/PMC8295921/ /pubmed/34307536 http://dx.doi.org/10.3389/fvets.2021.697479 Text en Copyright © 2021 Helms, Balogh, Franklin-Guild, Lahmers, Caswell and Cecere. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Helms, Alyssa B.
Balogh, Orsolya
Franklin-Guild, Rebecca
Lahmers, Kevin
Caswell, Clayton C.
Cecere, Julie T.
Presumptive Identification of Smooth Brucella Strain Antibodies in Canines
title Presumptive Identification of Smooth Brucella Strain Antibodies in Canines
title_full Presumptive Identification of Smooth Brucella Strain Antibodies in Canines
title_fullStr Presumptive Identification of Smooth Brucella Strain Antibodies in Canines
title_full_unstemmed Presumptive Identification of Smooth Brucella Strain Antibodies in Canines
title_short Presumptive Identification of Smooth Brucella Strain Antibodies in Canines
title_sort presumptive identification of smooth brucella strain antibodies in canines
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295921/
https://www.ncbi.nlm.nih.gov/pubmed/34307536
http://dx.doi.org/10.3389/fvets.2021.697479
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