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Pancreatitis-Associated Extracellular Vesicle Identification through an Allosteric Probe-Initiated Cascade Amplification System

[Image: see text] As an emerging class of noninvasive biomarkers, accurate quantification of circulating extracellular vesicles (EVs) is essential to both the basic biological research and early diagnosis of pancreatitis. We report here an allosteric probe-initiated cascade amplification system for...

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Autores principales: Zhou, Yuan, Yu, Feng, Cheng, Heng, Ning, Linhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296005/
https://www.ncbi.nlm.nih.gov/pubmed/34308013
http://dx.doi.org/10.1021/acsomega.0c06334
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author Zhou, Yuan
Yu, Feng
Cheng, Heng
Ning, Linhong
author_facet Zhou, Yuan
Yu, Feng
Cheng, Heng
Ning, Linhong
author_sort Zhou, Yuan
collection PubMed
description [Image: see text] As an emerging class of noninvasive biomarkers, accurate quantification of circulating extracellular vesicles (EVs) is essential to both the basic biological research and early diagnosis of pancreatitis. We report here an allosteric probe-initiated cascade amplification system for a highly sensitive detection of serum-circulating EVs. In this method, the special recognition of EVs by the allosteric probes triggers allosterism of the probe and thus induces the release of a signal amplification initiator. Through the following multiple rounds of cascade amplifications, a large number of fluorescence moiety are released, generating an enhanced fluorescence signal. This method exhibits a large dynamic range of 5 orders of magnitude. In addition, this strategy could also be performed under isothermal conditions in a wash-free way, indicating its potential applications in early diagnosis and prognosis of pancreatitis.
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spelling pubmed-82960052021-07-23 Pancreatitis-Associated Extracellular Vesicle Identification through an Allosteric Probe-Initiated Cascade Amplification System Zhou, Yuan Yu, Feng Cheng, Heng Ning, Linhong ACS Omega [Image: see text] As an emerging class of noninvasive biomarkers, accurate quantification of circulating extracellular vesicles (EVs) is essential to both the basic biological research and early diagnosis of pancreatitis. We report here an allosteric probe-initiated cascade amplification system for a highly sensitive detection of serum-circulating EVs. In this method, the special recognition of EVs by the allosteric probes triggers allosterism of the probe and thus induces the release of a signal amplification initiator. Through the following multiple rounds of cascade amplifications, a large number of fluorescence moiety are released, generating an enhanced fluorescence signal. This method exhibits a large dynamic range of 5 orders of magnitude. In addition, this strategy could also be performed under isothermal conditions in a wash-free way, indicating its potential applications in early diagnosis and prognosis of pancreatitis. American Chemical Society 2021-07-06 /pmc/articles/PMC8296005/ /pubmed/34308013 http://dx.doi.org/10.1021/acsomega.0c06334 Text en © 2021 The Authors. Published by American Chemical Society Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Zhou, Yuan
Yu, Feng
Cheng, Heng
Ning, Linhong
Pancreatitis-Associated Extracellular Vesicle Identification through an Allosteric Probe-Initiated Cascade Amplification System
title Pancreatitis-Associated Extracellular Vesicle Identification through an Allosteric Probe-Initiated Cascade Amplification System
title_full Pancreatitis-Associated Extracellular Vesicle Identification through an Allosteric Probe-Initiated Cascade Amplification System
title_fullStr Pancreatitis-Associated Extracellular Vesicle Identification through an Allosteric Probe-Initiated Cascade Amplification System
title_full_unstemmed Pancreatitis-Associated Extracellular Vesicle Identification through an Allosteric Probe-Initiated Cascade Amplification System
title_short Pancreatitis-Associated Extracellular Vesicle Identification through an Allosteric Probe-Initiated Cascade Amplification System
title_sort pancreatitis-associated extracellular vesicle identification through an allosteric probe-initiated cascade amplification system
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296005/
https://www.ncbi.nlm.nih.gov/pubmed/34308013
http://dx.doi.org/10.1021/acsomega.0c06334
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