Cargando…

Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay

Contaminants of Emerging Concern (CECs) with estrogenic or estrogenic-like activity have been increasingly detected in aquatic environments and have been an issue of global concern due to their potential negative effects on wildlife and human health. This study used the MCF-7 cell proliferation assa...

Descripción completa

Detalles Bibliográficos
Autores principales: Elfadul, Rehab, Jesien, Roman, Elnabawi, Ahmed, Chigbu, Paulinus, Ishaque, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296043/
https://www.ncbi.nlm.nih.gov/pubmed/34207818
http://dx.doi.org/10.3390/ijerph18126254
_version_ 1783725546824269824
author Elfadul, Rehab
Jesien, Roman
Elnabawi, Ahmed
Chigbu, Paulinus
Ishaque, Ali
author_facet Elfadul, Rehab
Jesien, Roman
Elnabawi, Ahmed
Chigbu, Paulinus
Ishaque, Ali
author_sort Elfadul, Rehab
collection PubMed
description Contaminants of Emerging Concern (CECs) with estrogenic or estrogenic-like activity have been increasingly detected in aquatic environments and have been an issue of global concern due to their potential negative effects on wildlife and human health. This study used the MCF-7 cell proliferation assay (E-Screen) to assess the estrogenic activity profiles in Maryland Coastal Bays (MCBs), a eutrophic system of estuaries impacted by human activities. Estrogenic activity was observed in all study sites tested. Water samples from MCBs increased MCF-7 cell proliferation above the negative control from 2.1-fold at site 8, located in Sinepuxent Bay close to the Ocean City Inlet, to 6.3-fold at site 6, located in Newport Bay. The proliferative effects of the sediment samples over the negative control ranged from 1.9-fold at the Assateague Island National Seashore site to 7.7-fold at the Public Landing site. Moreover, elevated cell proliferation (p < 0.05) was observed when cells were co-exposed with 17ß-Estradiol (E2), while reduction in cell proliferation was observed when cells were co-exposed with the antagonist ICI 182, 780 suggesting that cell proliferative effects were primarily mediated by the estrogen receptor (ER). These results suggest the occurrence of some estrogenic or hormonal-like compounds in the MCBs and are consistent with our previous findings based on vitellogenin analyses.
format Online
Article
Text
id pubmed-8296043
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-82960432021-07-23 Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay Elfadul, Rehab Jesien, Roman Elnabawi, Ahmed Chigbu, Paulinus Ishaque, Ali Int J Environ Res Public Health Article Contaminants of Emerging Concern (CECs) with estrogenic or estrogenic-like activity have been increasingly detected in aquatic environments and have been an issue of global concern due to their potential negative effects on wildlife and human health. This study used the MCF-7 cell proliferation assay (E-Screen) to assess the estrogenic activity profiles in Maryland Coastal Bays (MCBs), a eutrophic system of estuaries impacted by human activities. Estrogenic activity was observed in all study sites tested. Water samples from MCBs increased MCF-7 cell proliferation above the negative control from 2.1-fold at site 8, located in Sinepuxent Bay close to the Ocean City Inlet, to 6.3-fold at site 6, located in Newport Bay. The proliferative effects of the sediment samples over the negative control ranged from 1.9-fold at the Assateague Island National Seashore site to 7.7-fold at the Public Landing site. Moreover, elevated cell proliferation (p < 0.05) was observed when cells were co-exposed with 17ß-Estradiol (E2), while reduction in cell proliferation was observed when cells were co-exposed with the antagonist ICI 182, 780 suggesting that cell proliferative effects were primarily mediated by the estrogen receptor (ER). These results suggest the occurrence of some estrogenic or hormonal-like compounds in the MCBs and are consistent with our previous findings based on vitellogenin analyses. MDPI 2021-06-09 /pmc/articles/PMC8296043/ /pubmed/34207818 http://dx.doi.org/10.3390/ijerph18126254 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Elfadul, Rehab
Jesien, Roman
Elnabawi, Ahmed
Chigbu, Paulinus
Ishaque, Ali
Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay
title Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay
title_full Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay
title_fullStr Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay
title_full_unstemmed Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay
title_short Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay
title_sort analysis of estrogenic activity in maryland coastal bays using the mcf-7 cell proliferation assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296043/
https://www.ncbi.nlm.nih.gov/pubmed/34207818
http://dx.doi.org/10.3390/ijerph18126254
work_keys_str_mv AT elfadulrehab analysisofestrogenicactivityinmarylandcoastalbaysusingthemcf7cellproliferationassay
AT jesienroman analysisofestrogenicactivityinmarylandcoastalbaysusingthemcf7cellproliferationassay
AT elnabawiahmed analysisofestrogenicactivityinmarylandcoastalbaysusingthemcf7cellproliferationassay
AT chigbupaulinus analysisofestrogenicactivityinmarylandcoastalbaysusingthemcf7cellproliferationassay
AT ishaqueali analysisofestrogenicactivityinmarylandcoastalbaysusingthemcf7cellproliferationassay