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Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons
Intermediate progenitors of both excitatory and inhibitory neurons, which can replenish neurons in the adult brain, were recently identified. However, the generation of intermediate progenitors of GABAergic inhibitory neurons (IPGNs) has not been studied in detail. Here, we characterized the spatiot...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297055/ https://www.ncbi.nlm.nih.gov/pubmed/34305510 http://dx.doi.org/10.3389/fnins.2021.607908 |
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author | Esumi, Shigeyuki Nasu, Makoto Kawauchi, Takeshi Miike, Koichiro Morooka, Kento Yanagawa, Yuchio Seki, Tatsunori Sakimura, Kenji Fukuda, Takaichi Tamamaki, Nobuaki |
author_facet | Esumi, Shigeyuki Nasu, Makoto Kawauchi, Takeshi Miike, Koichiro Morooka, Kento Yanagawa, Yuchio Seki, Tatsunori Sakimura, Kenji Fukuda, Takaichi Tamamaki, Nobuaki |
author_sort | Esumi, Shigeyuki |
collection | PubMed |
description | Intermediate progenitors of both excitatory and inhibitory neurons, which can replenish neurons in the adult brain, were recently identified. However, the generation of intermediate progenitors of GABAergic inhibitory neurons (IPGNs) has not been studied in detail. Here, we characterized the spatiotemporal distribution of IPGNs in mouse cerebral cortex. IPGNs generated neurons during both embryonic and postnatal stages, but the embryonic IPGNs were more proliferative. Our lineage tracing analyses showed that the embryonically proliferating IPGNs tended to localize to the superficial layers rather than the deep cortical layers at 3 weeks after birth. We also found that embryonic IPGNs derived from the medial and caudal ganglionic eminence (CGE) but more than half of the embryonic IPGNs were derived from the CGE and broadly distributed in the cerebral cortex. Taken together, our data indicate that the broadly located IPGNs during embryonic and postnatal stages exhibit a different proliferative property and layer distribution. |
format | Online Article Text |
id | pubmed-8297055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82970552021-07-23 Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons Esumi, Shigeyuki Nasu, Makoto Kawauchi, Takeshi Miike, Koichiro Morooka, Kento Yanagawa, Yuchio Seki, Tatsunori Sakimura, Kenji Fukuda, Takaichi Tamamaki, Nobuaki Front Neurosci Neuroscience Intermediate progenitors of both excitatory and inhibitory neurons, which can replenish neurons in the adult brain, were recently identified. However, the generation of intermediate progenitors of GABAergic inhibitory neurons (IPGNs) has not been studied in detail. Here, we characterized the spatiotemporal distribution of IPGNs in mouse cerebral cortex. IPGNs generated neurons during both embryonic and postnatal stages, but the embryonic IPGNs were more proliferative. Our lineage tracing analyses showed that the embryonically proliferating IPGNs tended to localize to the superficial layers rather than the deep cortical layers at 3 weeks after birth. We also found that embryonic IPGNs derived from the medial and caudal ganglionic eminence (CGE) but more than half of the embryonic IPGNs were derived from the CGE and broadly distributed in the cerebral cortex. Taken together, our data indicate that the broadly located IPGNs during embryonic and postnatal stages exhibit a different proliferative property and layer distribution. Frontiers Media S.A. 2021-07-08 /pmc/articles/PMC8297055/ /pubmed/34305510 http://dx.doi.org/10.3389/fnins.2021.607908 Text en Copyright © 2021 Esumi, Nasu, Kawauchi, Miike, Morooka, Yanagawa, Seki, Sakimura, Fukuda and Tamamaki. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Esumi, Shigeyuki Nasu, Makoto Kawauchi, Takeshi Miike, Koichiro Morooka, Kento Yanagawa, Yuchio Seki, Tatsunori Sakimura, Kenji Fukuda, Takaichi Tamamaki, Nobuaki Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons |
title | Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons |
title_full | Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons |
title_fullStr | Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons |
title_full_unstemmed | Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons |
title_short | Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons |
title_sort | characterization and stage-dependent lineage analysis of intermediate progenitors of cortical gabaergic interneurons |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297055/ https://www.ncbi.nlm.nih.gov/pubmed/34305510 http://dx.doi.org/10.3389/fnins.2021.607908 |
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