Cargando…

Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons

Intermediate progenitors of both excitatory and inhibitory neurons, which can replenish neurons in the adult brain, were recently identified. However, the generation of intermediate progenitors of GABAergic inhibitory neurons (IPGNs) has not been studied in detail. Here, we characterized the spatiot...

Descripción completa

Detalles Bibliográficos
Autores principales: Esumi, Shigeyuki, Nasu, Makoto, Kawauchi, Takeshi, Miike, Koichiro, Morooka, Kento, Yanagawa, Yuchio, Seki, Tatsunori, Sakimura, Kenji, Fukuda, Takaichi, Tamamaki, Nobuaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297055/
https://www.ncbi.nlm.nih.gov/pubmed/34305510
http://dx.doi.org/10.3389/fnins.2021.607908
_version_ 1783725769841704960
author Esumi, Shigeyuki
Nasu, Makoto
Kawauchi, Takeshi
Miike, Koichiro
Morooka, Kento
Yanagawa, Yuchio
Seki, Tatsunori
Sakimura, Kenji
Fukuda, Takaichi
Tamamaki, Nobuaki
author_facet Esumi, Shigeyuki
Nasu, Makoto
Kawauchi, Takeshi
Miike, Koichiro
Morooka, Kento
Yanagawa, Yuchio
Seki, Tatsunori
Sakimura, Kenji
Fukuda, Takaichi
Tamamaki, Nobuaki
author_sort Esumi, Shigeyuki
collection PubMed
description Intermediate progenitors of both excitatory and inhibitory neurons, which can replenish neurons in the adult brain, were recently identified. However, the generation of intermediate progenitors of GABAergic inhibitory neurons (IPGNs) has not been studied in detail. Here, we characterized the spatiotemporal distribution of IPGNs in mouse cerebral cortex. IPGNs generated neurons during both embryonic and postnatal stages, but the embryonic IPGNs were more proliferative. Our lineage tracing analyses showed that the embryonically proliferating IPGNs tended to localize to the superficial layers rather than the deep cortical layers at 3 weeks after birth. We also found that embryonic IPGNs derived from the medial and caudal ganglionic eminence (CGE) but more than half of the embryonic IPGNs were derived from the CGE and broadly distributed in the cerebral cortex. Taken together, our data indicate that the broadly located IPGNs during embryonic and postnatal stages exhibit a different proliferative property and layer distribution.
format Online
Article
Text
id pubmed-8297055
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82970552021-07-23 Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons Esumi, Shigeyuki Nasu, Makoto Kawauchi, Takeshi Miike, Koichiro Morooka, Kento Yanagawa, Yuchio Seki, Tatsunori Sakimura, Kenji Fukuda, Takaichi Tamamaki, Nobuaki Front Neurosci Neuroscience Intermediate progenitors of both excitatory and inhibitory neurons, which can replenish neurons in the adult brain, were recently identified. However, the generation of intermediate progenitors of GABAergic inhibitory neurons (IPGNs) has not been studied in detail. Here, we characterized the spatiotemporal distribution of IPGNs in mouse cerebral cortex. IPGNs generated neurons during both embryonic and postnatal stages, but the embryonic IPGNs were more proliferative. Our lineage tracing analyses showed that the embryonically proliferating IPGNs tended to localize to the superficial layers rather than the deep cortical layers at 3 weeks after birth. We also found that embryonic IPGNs derived from the medial and caudal ganglionic eminence (CGE) but more than half of the embryonic IPGNs were derived from the CGE and broadly distributed in the cerebral cortex. Taken together, our data indicate that the broadly located IPGNs during embryonic and postnatal stages exhibit a different proliferative property and layer distribution. Frontiers Media S.A. 2021-07-08 /pmc/articles/PMC8297055/ /pubmed/34305510 http://dx.doi.org/10.3389/fnins.2021.607908 Text en Copyright © 2021 Esumi, Nasu, Kawauchi, Miike, Morooka, Yanagawa, Seki, Sakimura, Fukuda and Tamamaki. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Esumi, Shigeyuki
Nasu, Makoto
Kawauchi, Takeshi
Miike, Koichiro
Morooka, Kento
Yanagawa, Yuchio
Seki, Tatsunori
Sakimura, Kenji
Fukuda, Takaichi
Tamamaki, Nobuaki
Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons
title Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons
title_full Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons
title_fullStr Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons
title_full_unstemmed Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons
title_short Characterization and Stage-Dependent Lineage Analysis of Intermediate Progenitors of Cortical GABAergic Interneurons
title_sort characterization and stage-dependent lineage analysis of intermediate progenitors of cortical gabaergic interneurons
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297055/
https://www.ncbi.nlm.nih.gov/pubmed/34305510
http://dx.doi.org/10.3389/fnins.2021.607908
work_keys_str_mv AT esumishigeyuki characterizationandstagedependentlineageanalysisofintermediateprogenitorsofcorticalgabaergicinterneurons
AT nasumakoto characterizationandstagedependentlineageanalysisofintermediateprogenitorsofcorticalgabaergicinterneurons
AT kawauchitakeshi characterizationandstagedependentlineageanalysisofintermediateprogenitorsofcorticalgabaergicinterneurons
AT miikekoichiro characterizationandstagedependentlineageanalysisofintermediateprogenitorsofcorticalgabaergicinterneurons
AT morookakento characterizationandstagedependentlineageanalysisofintermediateprogenitorsofcorticalgabaergicinterneurons
AT yanagawayuchio characterizationandstagedependentlineageanalysisofintermediateprogenitorsofcorticalgabaergicinterneurons
AT sekitatsunori characterizationandstagedependentlineageanalysisofintermediateprogenitorsofcorticalgabaergicinterneurons
AT sakimurakenji characterizationandstagedependentlineageanalysisofintermediateprogenitorsofcorticalgabaergicinterneurons
AT fukudatakaichi characterizationandstagedependentlineageanalysisofintermediateprogenitorsofcorticalgabaergicinterneurons
AT tamamakinobuaki characterizationandstagedependentlineageanalysisofintermediateprogenitorsofcorticalgabaergicinterneurons