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An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth

Fish muscle growth is a complex process regulated by multiple pathways, resulting on the net accumulation of proteins and the activation of myogenic progenitor cells. Around 350–320 million years ago, teleost fish went through a specific whole genome duplication (WGD) that expanded the existent gene...

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Autores principales: Duran, Bruno Oliveira Silva, Garcia de la serrana, Daniel, Zanella, Bruna Tereza Thomazini, Perez, Erika Stefani, Mareco, Edson Assunção, Santos, Vander Bruno, Carvalho, Robson Francisco, Dal-Pai-Silva, Maeli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297816/
https://www.ncbi.nlm.nih.gov/pubmed/34293047
http://dx.doi.org/10.1371/journal.pone.0255006
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author Duran, Bruno Oliveira Silva
Garcia de la serrana, Daniel
Zanella, Bruna Tereza Thomazini
Perez, Erika Stefani
Mareco, Edson Assunção
Santos, Vander Bruno
Carvalho, Robson Francisco
Dal-Pai-Silva, Maeli
author_facet Duran, Bruno Oliveira Silva
Garcia de la serrana, Daniel
Zanella, Bruna Tereza Thomazini
Perez, Erika Stefani
Mareco, Edson Assunção
Santos, Vander Bruno
Carvalho, Robson Francisco
Dal-Pai-Silva, Maeli
author_sort Duran, Bruno Oliveira Silva
collection PubMed
description Fish muscle growth is a complex process regulated by multiple pathways, resulting on the net accumulation of proteins and the activation of myogenic progenitor cells. Around 350–320 million years ago, teleost fish went through a specific whole genome duplication (WGD) that expanded the existent gene repertoire. Duplicated genes can be retained by different molecular mechanisms such as subfunctionalization, neofunctionalization or redundancy, each one with different functional implications. While the great majority of ohnolog genes have been identified in the teleost genomes, the effect of gene duplication in the fish physiology is still not well characterized. In the present study we studied the effect of WGD on the transcription of the duplicated components controlling muscle growth. We compared the expression of lineage-specific ohnologs related to myogenesis and protein balance in the fast-skeletal muscle of pacus (Piaractus mesopotamicus—Ostariophysi) and Nile tilapias (Oreochromis niloticus—Acanthopterygii) fasted for 4 days and refed for 3 days. We studied the expression of 20 ohnologs and found that in the great majority of cases, duplicated genes had similar expression profiles in response to fasting and refeeding, indicating that their functions during growth have been conserved during the period after the WGD. Our results suggest that redundancy might play a more important role in the retention of ohnologs of regulatory pathways than initially thought. Also, comparison to non-duplicated orthologs showed that it might not be uncommon for the duplicated genes to gain or loss new regulatory elements simultaneously. Overall, several of duplicated ohnologs have similar transcription profiles in response to pro-growth signals suggesting that evolution tends to conserve ohnolog regulation during muscle development and that in the majority of ohnologs related to muscle growth their functions might be very similar.
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spelling pubmed-82978162021-07-31 An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth Duran, Bruno Oliveira Silva Garcia de la serrana, Daniel Zanella, Bruna Tereza Thomazini Perez, Erika Stefani Mareco, Edson Assunção Santos, Vander Bruno Carvalho, Robson Francisco Dal-Pai-Silva, Maeli PLoS One Research Article Fish muscle growth is a complex process regulated by multiple pathways, resulting on the net accumulation of proteins and the activation of myogenic progenitor cells. Around 350–320 million years ago, teleost fish went through a specific whole genome duplication (WGD) that expanded the existent gene repertoire. Duplicated genes can be retained by different molecular mechanisms such as subfunctionalization, neofunctionalization or redundancy, each one with different functional implications. While the great majority of ohnolog genes have been identified in the teleost genomes, the effect of gene duplication in the fish physiology is still not well characterized. In the present study we studied the effect of WGD on the transcription of the duplicated components controlling muscle growth. We compared the expression of lineage-specific ohnologs related to myogenesis and protein balance in the fast-skeletal muscle of pacus (Piaractus mesopotamicus—Ostariophysi) and Nile tilapias (Oreochromis niloticus—Acanthopterygii) fasted for 4 days and refed for 3 days. We studied the expression of 20 ohnologs and found that in the great majority of cases, duplicated genes had similar expression profiles in response to fasting and refeeding, indicating that their functions during growth have been conserved during the period after the WGD. Our results suggest that redundancy might play a more important role in the retention of ohnologs of regulatory pathways than initially thought. Also, comparison to non-duplicated orthologs showed that it might not be uncommon for the duplicated genes to gain or loss new regulatory elements simultaneously. Overall, several of duplicated ohnologs have similar transcription profiles in response to pro-growth signals suggesting that evolution tends to conserve ohnolog regulation during muscle development and that in the majority of ohnologs related to muscle growth their functions might be very similar. Public Library of Science 2021-07-22 /pmc/articles/PMC8297816/ /pubmed/34293047 http://dx.doi.org/10.1371/journal.pone.0255006 Text en © 2021 Duran et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Duran, Bruno Oliveira Silva
Garcia de la serrana, Daniel
Zanella, Bruna Tereza Thomazini
Perez, Erika Stefani
Mareco, Edson Assunção
Santos, Vander Bruno
Carvalho, Robson Francisco
Dal-Pai-Silva, Maeli
An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth
title An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth
title_full An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth
title_fullStr An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth
title_full_unstemmed An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth
title_short An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth
title_sort insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297816/
https://www.ncbi.nlm.nih.gov/pubmed/34293047
http://dx.doi.org/10.1371/journal.pone.0255006
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