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Estimating the effect of tracking tag weight on insect movement using video analysis: A case study with a flightless orthopteran

In this study, we describe an inexpensive and rapid method of using video analysis and identity tracking to measure the effects of tag weight on insect movement. In a laboratory experiment, we assessed the tag weight and associated context-dependent effects on movement, choosing temperature as a fac...

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Autores principales: Kaláb, Oto, Musiolek, David, Rusnok, Pavel, Hurtik, Petr, Tomis, Martin, Kočárek, Petr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297838/
https://www.ncbi.nlm.nih.gov/pubmed/34293059
http://dx.doi.org/10.1371/journal.pone.0255117
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author Kaláb, Oto
Musiolek, David
Rusnok, Pavel
Hurtik, Petr
Tomis, Martin
Kočárek, Petr
author_facet Kaláb, Oto
Musiolek, David
Rusnok, Pavel
Hurtik, Petr
Tomis, Martin
Kočárek, Petr
author_sort Kaláb, Oto
collection PubMed
description In this study, we describe an inexpensive and rapid method of using video analysis and identity tracking to measure the effects of tag weight on insect movement. In a laboratory experiment, we assessed the tag weight and associated context-dependent effects on movement, choosing temperature as a factor known to affect insect movement and behavior. We recorded the movements of groups of flightless adult crickets Gryllus locorojo (Orthoptera:Gryllidae) as affected by no tag (control); by light, medium, or heavy tags (198.7, 549.2, and 758.6 mg, respectively); and by low, intermediate, or high temperatures (19.5, 24.0, and 28.3°C, respectively). Each individual in each group was weighed before recording and was recorded for 3 consecutive days. The mean (± SD) tag mass expressed as a percentage of body mass before the first recording was 26.8 ± 3.7% with light tags, 72 ± 11.2% with medium tags, and 101.9 ± 13.5% with heavy tags. We found that the influence of tag weight strongly depended on temperature, and that the negative effects on movement generally increased with tag weight. At the low temperature, nearly all movement properties were negatively influenced. At the intermediate and high temperatures, the light and medium tags did not affect any of the movement properties. The continuous 3-day tag load reduced the average movement speed only for crickets with heavy tags. Based on our results, we recommend that researchers consider or investigate the possible effects of tags before conducting any experiment with tags in order to avoid obtaining biased results.
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spelling pubmed-82978382021-07-31 Estimating the effect of tracking tag weight on insect movement using video analysis: A case study with a flightless orthopteran Kaláb, Oto Musiolek, David Rusnok, Pavel Hurtik, Petr Tomis, Martin Kočárek, Petr PLoS One Research Article In this study, we describe an inexpensive and rapid method of using video analysis and identity tracking to measure the effects of tag weight on insect movement. In a laboratory experiment, we assessed the tag weight and associated context-dependent effects on movement, choosing temperature as a factor known to affect insect movement and behavior. We recorded the movements of groups of flightless adult crickets Gryllus locorojo (Orthoptera:Gryllidae) as affected by no tag (control); by light, medium, or heavy tags (198.7, 549.2, and 758.6 mg, respectively); and by low, intermediate, or high temperatures (19.5, 24.0, and 28.3°C, respectively). Each individual in each group was weighed before recording and was recorded for 3 consecutive days. The mean (± SD) tag mass expressed as a percentage of body mass before the first recording was 26.8 ± 3.7% with light tags, 72 ± 11.2% with medium tags, and 101.9 ± 13.5% with heavy tags. We found that the influence of tag weight strongly depended on temperature, and that the negative effects on movement generally increased with tag weight. At the low temperature, nearly all movement properties were negatively influenced. At the intermediate and high temperatures, the light and medium tags did not affect any of the movement properties. The continuous 3-day tag load reduced the average movement speed only for crickets with heavy tags. Based on our results, we recommend that researchers consider or investigate the possible effects of tags before conducting any experiment with tags in order to avoid obtaining biased results. Public Library of Science 2021-07-22 /pmc/articles/PMC8297838/ /pubmed/34293059 http://dx.doi.org/10.1371/journal.pone.0255117 Text en © 2021 Kaláb et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kaláb, Oto
Musiolek, David
Rusnok, Pavel
Hurtik, Petr
Tomis, Martin
Kočárek, Petr
Estimating the effect of tracking tag weight on insect movement using video analysis: A case study with a flightless orthopteran
title Estimating the effect of tracking tag weight on insect movement using video analysis: A case study with a flightless orthopteran
title_full Estimating the effect of tracking tag weight on insect movement using video analysis: A case study with a flightless orthopteran
title_fullStr Estimating the effect of tracking tag weight on insect movement using video analysis: A case study with a flightless orthopteran
title_full_unstemmed Estimating the effect of tracking tag weight on insect movement using video analysis: A case study with a flightless orthopteran
title_short Estimating the effect of tracking tag weight on insect movement using video analysis: A case study with a flightless orthopteran
title_sort estimating the effect of tracking tag weight on insect movement using video analysis: a case study with a flightless orthopteran
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297838/
https://www.ncbi.nlm.nih.gov/pubmed/34293059
http://dx.doi.org/10.1371/journal.pone.0255117
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