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Characterization of E93 in neometabolous thrips Frankliniella occidentalis and Haplothrips brevitubus
Insect metamorphosis into an adult occurs after the juvenile hormone (JH) titer decreases at the end of the juvenile stage. This generally coincides with decreased transcript levels of JH-response transcription factors Krüppel homolog 1 (Kr-h1) and broad (br), and increased transcript levels of the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297894/ https://www.ncbi.nlm.nih.gov/pubmed/34293026 http://dx.doi.org/10.1371/journal.pone.0254963 |
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author | Suzuki, Youhei Shiotsuki, Takahiro Jouraku, Akiya Miura, Ken Minakuchi, Chieka |
author_facet | Suzuki, Youhei Shiotsuki, Takahiro Jouraku, Akiya Miura, Ken Minakuchi, Chieka |
author_sort | Suzuki, Youhei |
collection | PubMed |
description | Insect metamorphosis into an adult occurs after the juvenile hormone (JH) titer decreases at the end of the juvenile stage. This generally coincides with decreased transcript levels of JH-response transcription factors Krüppel homolog 1 (Kr-h1) and broad (br), and increased transcript levels of the adult specifier E93. Thrips (Thysanoptera) develop through inactive and non-feeding stages referred to as “propupa” and “pupa”, and this type of distinctive metamorphosis is called neometaboly. To understand the mechanisms of hormonal regulation in thrips metamorphosis, we previously analyzed the transcript levels of Kr-h1 and br in two thrips species, Frankliniella occidentalis (Thripidae) and Haplothrips brevitubus (Phlaeothripidae). In both species, the transcript levels of Kr-h1 and br decreased in the “propupal” and “pupal” stages, and their transcription was upregulated by exogenous JH mimic treatment. Here we analyzed the developmental profiles of E93 in these two thrips species. Quantitative RT-PCR revealed that E93 expression started to increase at the end of the larval stage in F. occidentalis and in the “propupal” stage of H. brevitubus, as Kr-h1 and br mRNA levels decreased. Treatment with an exogenous JH mimic at the onset of metamorphosis prevented pupal-adult transition and caused repression of E93. These results indicated that E93 is involved in adult differentiation after JH titer decreases at the end of the larval stage of thrips. By comparing the expression profiles of Kr-h1, br, and E93 among insect species, we propose that the “propupal” and “pupal” stages of thrips have some similarities with the holometabolous prepupal and pupal stages, respectively. |
format | Online Article Text |
id | pubmed-8297894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-82978942021-07-31 Characterization of E93 in neometabolous thrips Frankliniella occidentalis and Haplothrips brevitubus Suzuki, Youhei Shiotsuki, Takahiro Jouraku, Akiya Miura, Ken Minakuchi, Chieka PLoS One Research Article Insect metamorphosis into an adult occurs after the juvenile hormone (JH) titer decreases at the end of the juvenile stage. This generally coincides with decreased transcript levels of JH-response transcription factors Krüppel homolog 1 (Kr-h1) and broad (br), and increased transcript levels of the adult specifier E93. Thrips (Thysanoptera) develop through inactive and non-feeding stages referred to as “propupa” and “pupa”, and this type of distinctive metamorphosis is called neometaboly. To understand the mechanisms of hormonal regulation in thrips metamorphosis, we previously analyzed the transcript levels of Kr-h1 and br in two thrips species, Frankliniella occidentalis (Thripidae) and Haplothrips brevitubus (Phlaeothripidae). In both species, the transcript levels of Kr-h1 and br decreased in the “propupal” and “pupal” stages, and their transcription was upregulated by exogenous JH mimic treatment. Here we analyzed the developmental profiles of E93 in these two thrips species. Quantitative RT-PCR revealed that E93 expression started to increase at the end of the larval stage in F. occidentalis and in the “propupal” stage of H. brevitubus, as Kr-h1 and br mRNA levels decreased. Treatment with an exogenous JH mimic at the onset of metamorphosis prevented pupal-adult transition and caused repression of E93. These results indicated that E93 is involved in adult differentiation after JH titer decreases at the end of the larval stage of thrips. By comparing the expression profiles of Kr-h1, br, and E93 among insect species, we propose that the “propupal” and “pupal” stages of thrips have some similarities with the holometabolous prepupal and pupal stages, respectively. Public Library of Science 2021-07-22 /pmc/articles/PMC8297894/ /pubmed/34293026 http://dx.doi.org/10.1371/journal.pone.0254963 Text en © 2021 Suzuki et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Suzuki, Youhei Shiotsuki, Takahiro Jouraku, Akiya Miura, Ken Minakuchi, Chieka Characterization of E93 in neometabolous thrips Frankliniella occidentalis and Haplothrips brevitubus |
title | Characterization of E93 in neometabolous thrips Frankliniella occidentalis and Haplothrips brevitubus |
title_full | Characterization of E93 in neometabolous thrips Frankliniella occidentalis and Haplothrips brevitubus |
title_fullStr | Characterization of E93 in neometabolous thrips Frankliniella occidentalis and Haplothrips brevitubus |
title_full_unstemmed | Characterization of E93 in neometabolous thrips Frankliniella occidentalis and Haplothrips brevitubus |
title_short | Characterization of E93 in neometabolous thrips Frankliniella occidentalis and Haplothrips brevitubus |
title_sort | characterization of e93 in neometabolous thrips frankliniella occidentalis and haplothrips brevitubus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297894/ https://www.ncbi.nlm.nih.gov/pubmed/34293026 http://dx.doi.org/10.1371/journal.pone.0254963 |
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