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In vivo recellularization of xenogeneic vascular grafts decellularized with high hydrostatic pressure method in a porcine carotid arterial interpose model

Autologous vascular grafts are widely used in revascularization surgeries for small caliber targets. However, the availability of autologous conduits might be limited due to prior surgeries or the quality of vessels. Xenogeneic decellularized vascular grafts from animals can potentially be a substit...

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Autores principales: Kurokawa, Shunji, Hashimoto, Yoshihide, Funamoto, Seiichi, Murata, Kozue, Yamashita, Akitatsu, Yamazaki, Kazuhiro, Ikeda, Tadashi, Minatoya, Kenji, Kishida, Akio, Masumoto, Hidetoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297896/
https://www.ncbi.nlm.nih.gov/pubmed/34292963
http://dx.doi.org/10.1371/journal.pone.0254160
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author Kurokawa, Shunji
Hashimoto, Yoshihide
Funamoto, Seiichi
Murata, Kozue
Yamashita, Akitatsu
Yamazaki, Kazuhiro
Ikeda, Tadashi
Minatoya, Kenji
Kishida, Akio
Masumoto, Hidetoshi
author_facet Kurokawa, Shunji
Hashimoto, Yoshihide
Funamoto, Seiichi
Murata, Kozue
Yamashita, Akitatsu
Yamazaki, Kazuhiro
Ikeda, Tadashi
Minatoya, Kenji
Kishida, Akio
Masumoto, Hidetoshi
author_sort Kurokawa, Shunji
collection PubMed
description Autologous vascular grafts are widely used in revascularization surgeries for small caliber targets. However, the availability of autologous conduits might be limited due to prior surgeries or the quality of vessels. Xenogeneic decellularized vascular grafts from animals can potentially be a substitute of autologous vascular grafts. Decellularization with high hydrostatic pressure (HHP) is reported to highly preserve extracellular matrix (ECM), creating feasible conditions for recellularization and vascular remodeling after implantation. In the present study, we conducted xenogeneic implantation of HHP-decellularized bovine vascular grafts from dorsalis pedis arteries to porcine carotid arteries and posteriorly evaluated graft patency, ECM preservation and recellularization. Avoiding damage of the luminal surface of the grafts from drying significantly during the surgical procedure increased the graft patency at 4 weeks after implantation (P = 0.0079). After the technical improvement, all grafts (N = 5) were patent with mild stenosis due to intimal hyperplasia at 4 weeks after implantation. Neither aneurysmal change nor massive thrombosis was observed, even without administration of anticoagulants nor anti-platelet agents. Elastica van Gieson and Sirius-red stainings revealed fair preservation of ECM proteins including elastin and collagen after implantation. The luminal surface of the grafts were thoroughly covered with von Willebrand factor-positive endothelium. Scanning electron microscopy of the luminal surface of implanted grafts exhibited a cobblestone-like endothelial cell layer which is similar to native vascular endothelium. Recellularization of the tunica media with alpha-smooth muscle actin-positive smooth muscle cells was partly observed. Thus, we confirmed that HHP-decellularized grafts are feasible for xenogeneic implantation accompanied by recellularization by recipient cells.
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spelling pubmed-82978962021-07-31 In vivo recellularization of xenogeneic vascular grafts decellularized with high hydrostatic pressure method in a porcine carotid arterial interpose model Kurokawa, Shunji Hashimoto, Yoshihide Funamoto, Seiichi Murata, Kozue Yamashita, Akitatsu Yamazaki, Kazuhiro Ikeda, Tadashi Minatoya, Kenji Kishida, Akio Masumoto, Hidetoshi PLoS One Research Article Autologous vascular grafts are widely used in revascularization surgeries for small caliber targets. However, the availability of autologous conduits might be limited due to prior surgeries or the quality of vessels. Xenogeneic decellularized vascular grafts from animals can potentially be a substitute of autologous vascular grafts. Decellularization with high hydrostatic pressure (HHP) is reported to highly preserve extracellular matrix (ECM), creating feasible conditions for recellularization and vascular remodeling after implantation. In the present study, we conducted xenogeneic implantation of HHP-decellularized bovine vascular grafts from dorsalis pedis arteries to porcine carotid arteries and posteriorly evaluated graft patency, ECM preservation and recellularization. Avoiding damage of the luminal surface of the grafts from drying significantly during the surgical procedure increased the graft patency at 4 weeks after implantation (P = 0.0079). After the technical improvement, all grafts (N = 5) were patent with mild stenosis due to intimal hyperplasia at 4 weeks after implantation. Neither aneurysmal change nor massive thrombosis was observed, even without administration of anticoagulants nor anti-platelet agents. Elastica van Gieson and Sirius-red stainings revealed fair preservation of ECM proteins including elastin and collagen after implantation. The luminal surface of the grafts were thoroughly covered with von Willebrand factor-positive endothelium. Scanning electron microscopy of the luminal surface of implanted grafts exhibited a cobblestone-like endothelial cell layer which is similar to native vascular endothelium. Recellularization of the tunica media with alpha-smooth muscle actin-positive smooth muscle cells was partly observed. Thus, we confirmed that HHP-decellularized grafts are feasible for xenogeneic implantation accompanied by recellularization by recipient cells. Public Library of Science 2021-07-22 /pmc/articles/PMC8297896/ /pubmed/34292963 http://dx.doi.org/10.1371/journal.pone.0254160 Text en © 2021 Kurokawa et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kurokawa, Shunji
Hashimoto, Yoshihide
Funamoto, Seiichi
Murata, Kozue
Yamashita, Akitatsu
Yamazaki, Kazuhiro
Ikeda, Tadashi
Minatoya, Kenji
Kishida, Akio
Masumoto, Hidetoshi
In vivo recellularization of xenogeneic vascular grafts decellularized with high hydrostatic pressure method in a porcine carotid arterial interpose model
title In vivo recellularization of xenogeneic vascular grafts decellularized with high hydrostatic pressure method in a porcine carotid arterial interpose model
title_full In vivo recellularization of xenogeneic vascular grafts decellularized with high hydrostatic pressure method in a porcine carotid arterial interpose model
title_fullStr In vivo recellularization of xenogeneic vascular grafts decellularized with high hydrostatic pressure method in a porcine carotid arterial interpose model
title_full_unstemmed In vivo recellularization of xenogeneic vascular grafts decellularized with high hydrostatic pressure method in a porcine carotid arterial interpose model
title_short In vivo recellularization of xenogeneic vascular grafts decellularized with high hydrostatic pressure method in a porcine carotid arterial interpose model
title_sort in vivo recellularization of xenogeneic vascular grafts decellularized with high hydrostatic pressure method in a porcine carotid arterial interpose model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297896/
https://www.ncbi.nlm.nih.gov/pubmed/34292963
http://dx.doi.org/10.1371/journal.pone.0254160
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