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Microsatellite Instability and Colorectal Cancer, Immunohistochemical and Molecular Evaluation by Using DNA Sequencing: A Single Center Experience

BACKGROUND & OBJECTIVE: Microsatellite instability is common in familial colorectal cancers. It can be tested by the molecular and immunohistochemical methods. There are very few studies which address comparing the clinicopathological characteristics of microsatellite stable (MSS) and microsatel...

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Autores principales: Geramizadeh, Bita, Bozorg-Ghalati, Farzaneh, Jafari, Firoozeh, Mirzai, Mitra, Jowkar, Zahra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Pathology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298054/
https://www.ncbi.nlm.nih.gov/pubmed/34306129
http://dx.doi.org/10.30699/ijp.2021.135222.2481
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author Geramizadeh, Bita
Bozorg-Ghalati, Farzaneh
Jafari, Firoozeh
Mirzai, Mitra
Jowkar, Zahra
author_facet Geramizadeh, Bita
Bozorg-Ghalati, Farzaneh
Jafari, Firoozeh
Mirzai, Mitra
Jowkar, Zahra
author_sort Geramizadeh, Bita
collection PubMed
description BACKGROUND & OBJECTIVE: Microsatellite instability is common in familial colorectal cancers. It can be tested by the molecular and immunohistochemical methods. There are very few studies which address comparing the clinicopathological characteristics of microsatellite stable (MSS) and microsatellite unstable (MSI) colorectal cancers from Iran. In this study, we aimed to evaluate the clinicopathological and immuno-histochemical findings of MSS and MSI colorectal cancers in our Center as the largest Center of gastrointestinal surgery and oncology in the South of Iran. We also compared the immunohistochemical method vs. molecular study using DNA sequencing. METHODS: For 5 years (2015-2019), 34 patients who underwent operation in the affiliated Hospitals of Shiraz University of Medical Sciences were clinically suspected to microsatellite instability (MSI). The molecular diagnostic tests with DNA sequencing were performed. Clinicopathological and immunohistochemical findings of MSI colorectal cancers were compared with those who were stable. RESULTS: In the South of Iran, MSI colorectal cancers were more common in males. These tumors were more common in the right side with more tendencies to produce mucin with lymphocytic infiltration. CONCLUSION: Immunohistochemistry would be a specific method for diagnosis of MSI colorectal cancers but may be associated with high rate of false negative results and of low sensitivity. Therefore, we recommend performing molecular studies by DNA sequencing in those colon cancers with clinical suspicion for MSI and negative immunohistochemical findings.
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spelling pubmed-82980542021-07-23 Microsatellite Instability and Colorectal Cancer, Immunohistochemical and Molecular Evaluation by Using DNA Sequencing: A Single Center Experience Geramizadeh, Bita Bozorg-Ghalati, Farzaneh Jafari, Firoozeh Mirzai, Mitra Jowkar, Zahra Iran J Pathol Original Article BACKGROUND & OBJECTIVE: Microsatellite instability is common in familial colorectal cancers. It can be tested by the molecular and immunohistochemical methods. There are very few studies which address comparing the clinicopathological characteristics of microsatellite stable (MSS) and microsatellite unstable (MSI) colorectal cancers from Iran. In this study, we aimed to evaluate the clinicopathological and immuno-histochemical findings of MSS and MSI colorectal cancers in our Center as the largest Center of gastrointestinal surgery and oncology in the South of Iran. We also compared the immunohistochemical method vs. molecular study using DNA sequencing. METHODS: For 5 years (2015-2019), 34 patients who underwent operation in the affiliated Hospitals of Shiraz University of Medical Sciences were clinically suspected to microsatellite instability (MSI). The molecular diagnostic tests with DNA sequencing were performed. Clinicopathological and immunohistochemical findings of MSI colorectal cancers were compared with those who were stable. RESULTS: In the South of Iran, MSI colorectal cancers were more common in males. These tumors were more common in the right side with more tendencies to produce mucin with lymphocytic infiltration. CONCLUSION: Immunohistochemistry would be a specific method for diagnosis of MSI colorectal cancers but may be associated with high rate of false negative results and of low sensitivity. Therefore, we recommend performing molecular studies by DNA sequencing in those colon cancers with clinical suspicion for MSI and negative immunohistochemical findings. Iranian Society of Pathology 2021 2021-05-05 /pmc/articles/PMC8298054/ /pubmed/34306129 http://dx.doi.org/10.30699/ijp.2021.135222.2481 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Geramizadeh, Bita
Bozorg-Ghalati, Farzaneh
Jafari, Firoozeh
Mirzai, Mitra
Jowkar, Zahra
Microsatellite Instability and Colorectal Cancer, Immunohistochemical and Molecular Evaluation by Using DNA Sequencing: A Single Center Experience
title Microsatellite Instability and Colorectal Cancer, Immunohistochemical and Molecular Evaluation by Using DNA Sequencing: A Single Center Experience
title_full Microsatellite Instability and Colorectal Cancer, Immunohistochemical and Molecular Evaluation by Using DNA Sequencing: A Single Center Experience
title_fullStr Microsatellite Instability and Colorectal Cancer, Immunohistochemical and Molecular Evaluation by Using DNA Sequencing: A Single Center Experience
title_full_unstemmed Microsatellite Instability and Colorectal Cancer, Immunohistochemical and Molecular Evaluation by Using DNA Sequencing: A Single Center Experience
title_short Microsatellite Instability and Colorectal Cancer, Immunohistochemical and Molecular Evaluation by Using DNA Sequencing: A Single Center Experience
title_sort microsatellite instability and colorectal cancer, immunohistochemical and molecular evaluation by using dna sequencing: a single center experience
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298054/
https://www.ncbi.nlm.nih.gov/pubmed/34306129
http://dx.doi.org/10.30699/ijp.2021.135222.2481
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