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Nitrogen Supply and Host-Plant Genotype Modulate the Transcriptomic Profile of Plasmodiophora brassicae

Nitrogen fertilization can affect the susceptibility of Brassica napus to the telluric pathogen Plasmodiophora brassicae. Our previous works highlighted that the influence of nitrogen can strongly vary regarding plant cultivar/pathogen strain combinations, but the underlying mechanisms are unknown....

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Autores principales: Gazengel, Kévin, Aigu, Yoann, Lariagon, Christine, Humeau, Mathilde, Gravot, Antoine, Manzanares-Dauleux, Maria J., Daval, Stéphanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298192/
https://www.ncbi.nlm.nih.gov/pubmed/34305867
http://dx.doi.org/10.3389/fmicb.2021.701067
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author Gazengel, Kévin
Aigu, Yoann
Lariagon, Christine
Humeau, Mathilde
Gravot, Antoine
Manzanares-Dauleux, Maria J.
Daval, Stéphanie
author_facet Gazengel, Kévin
Aigu, Yoann
Lariagon, Christine
Humeau, Mathilde
Gravot, Antoine
Manzanares-Dauleux, Maria J.
Daval, Stéphanie
author_sort Gazengel, Kévin
collection PubMed
description Nitrogen fertilization can affect the susceptibility of Brassica napus to the telluric pathogen Plasmodiophora brassicae. Our previous works highlighted that the influence of nitrogen can strongly vary regarding plant cultivar/pathogen strain combinations, but the underlying mechanisms are unknown. The present work aims to explore how nitrogen supply can affect the molecular physiology of P. brassicae through its life epidemiological cycle. A time-course transcriptome experiment was conducted to study the interaction, under two conditions of nitrogen supply, between isolate eH and two B. napus genotypes (Yudal and HD-018), harboring (or not harboring) low nitrogen-conditional resistance toward this isolate (respectively). P. brassicae transcriptional patterns were modulated by nitrogen supply, these modulations being dependent on both host-plant genotype and kinetic time. Functional analysis allowed the identification of P. brassicae genes expressed during the secondary phase of infection, which may play a role in the reduction of Yudal disease symptoms in low-nitrogen conditions. Candidate genes included pathogenicity-related genes (“NUDIX,” “carboxypeptidase,” and “NEP-proteins”) and genes associated to obligate biotrophic functions of P. brassicae. This work illustrates the importance of considering pathogen’s physiological responses to get a better understanding of the influence of abiotic factors on clubroot resistance/susceptibility.
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spelling pubmed-82981922021-07-24 Nitrogen Supply and Host-Plant Genotype Modulate the Transcriptomic Profile of Plasmodiophora brassicae Gazengel, Kévin Aigu, Yoann Lariagon, Christine Humeau, Mathilde Gravot, Antoine Manzanares-Dauleux, Maria J. Daval, Stéphanie Front Microbiol Microbiology Nitrogen fertilization can affect the susceptibility of Brassica napus to the telluric pathogen Plasmodiophora brassicae. Our previous works highlighted that the influence of nitrogen can strongly vary regarding plant cultivar/pathogen strain combinations, but the underlying mechanisms are unknown. The present work aims to explore how nitrogen supply can affect the molecular physiology of P. brassicae through its life epidemiological cycle. A time-course transcriptome experiment was conducted to study the interaction, under two conditions of nitrogen supply, between isolate eH and two B. napus genotypes (Yudal and HD-018), harboring (or not harboring) low nitrogen-conditional resistance toward this isolate (respectively). P. brassicae transcriptional patterns were modulated by nitrogen supply, these modulations being dependent on both host-plant genotype and kinetic time. Functional analysis allowed the identification of P. brassicae genes expressed during the secondary phase of infection, which may play a role in the reduction of Yudal disease symptoms in low-nitrogen conditions. Candidate genes included pathogenicity-related genes (“NUDIX,” “carboxypeptidase,” and “NEP-proteins”) and genes associated to obligate biotrophic functions of P. brassicae. This work illustrates the importance of considering pathogen’s physiological responses to get a better understanding of the influence of abiotic factors on clubroot resistance/susceptibility. Frontiers Media S.A. 2021-07-08 /pmc/articles/PMC8298192/ /pubmed/34305867 http://dx.doi.org/10.3389/fmicb.2021.701067 Text en Copyright © 2021 Gazengel, Aigu, Lariagon, Humeau, Gravot, Manzanares-Dauleux and Daval. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gazengel, Kévin
Aigu, Yoann
Lariagon, Christine
Humeau, Mathilde
Gravot, Antoine
Manzanares-Dauleux, Maria J.
Daval, Stéphanie
Nitrogen Supply and Host-Plant Genotype Modulate the Transcriptomic Profile of Plasmodiophora brassicae
title Nitrogen Supply and Host-Plant Genotype Modulate the Transcriptomic Profile of Plasmodiophora brassicae
title_full Nitrogen Supply and Host-Plant Genotype Modulate the Transcriptomic Profile of Plasmodiophora brassicae
title_fullStr Nitrogen Supply and Host-Plant Genotype Modulate the Transcriptomic Profile of Plasmodiophora brassicae
title_full_unstemmed Nitrogen Supply and Host-Plant Genotype Modulate the Transcriptomic Profile of Plasmodiophora brassicae
title_short Nitrogen Supply and Host-Plant Genotype Modulate the Transcriptomic Profile of Plasmodiophora brassicae
title_sort nitrogen supply and host-plant genotype modulate the transcriptomic profile of plasmodiophora brassicae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298192/
https://www.ncbi.nlm.nih.gov/pubmed/34305867
http://dx.doi.org/10.3389/fmicb.2021.701067
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