A single dose of replication-competent VSV-vectored vaccine expressing SARS-CoV-2 S1 protects against virus replication in a hamster model of severe COVID-19

The development of effective countermeasures against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the agent responsible for the COVID-19 pandemic, is a priority. We designed and produced ConVac, a replication-competent vesicular stomatitis virus (VSV) vaccine vector that expresses t...

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Autores principales: Malherbe, Delphine C., Kurup, Drishya, Wirblich, Christoph, Ronk, Adam J., Mire, Chad, Kuzmina, Natalia, Shaik, Noor, Periasamy, Sivakumar, Hyde, Matthew A., Williams, Julie M., Shi, Pei-Yong, Schnell, Matthias J., Bukreyev, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298481/
https://www.ncbi.nlm.nih.gov/pubmed/34294728
http://dx.doi.org/10.1038/s41541-021-00352-1
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author Malherbe, Delphine C.
Kurup, Drishya
Wirblich, Christoph
Ronk, Adam J.
Mire, Chad
Kuzmina, Natalia
Shaik, Noor
Periasamy, Sivakumar
Hyde, Matthew A.
Williams, Julie M.
Shi, Pei-Yong
Schnell, Matthias J.
Bukreyev, Alexander
author_facet Malherbe, Delphine C.
Kurup, Drishya
Wirblich, Christoph
Ronk, Adam J.
Mire, Chad
Kuzmina, Natalia
Shaik, Noor
Periasamy, Sivakumar
Hyde, Matthew A.
Williams, Julie M.
Shi, Pei-Yong
Schnell, Matthias J.
Bukreyev, Alexander
author_sort Malherbe, Delphine C.
collection PubMed
description The development of effective countermeasures against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the agent responsible for the COVID-19 pandemic, is a priority. We designed and produced ConVac, a replication-competent vesicular stomatitis virus (VSV) vaccine vector that expresses the S1 subunit of SARS-CoV-2 spike protein. We used golden Syrian hamsters as animal models of severe COVID-19 to test the efficacy of the ConVac vaccine. A single vaccine dose elicited high levels of SARS-CoV-2 specific binding and neutralizing antibodies; following intranasal challenge with SARS-CoV-2, animals were protected from weight loss and viral replication in the lungs. No enhanced pathology was observed in vaccinated animals upon challenge, but some inflammation was still detected. The data indicate rapid control of SARS-CoV-2 replication by the S1-based VSV-vectored SARS-CoV-2 ConVac vaccine.
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spelling pubmed-82984812021-08-05 A single dose of replication-competent VSV-vectored vaccine expressing SARS-CoV-2 S1 protects against virus replication in a hamster model of severe COVID-19 Malherbe, Delphine C. Kurup, Drishya Wirblich, Christoph Ronk, Adam J. Mire, Chad Kuzmina, Natalia Shaik, Noor Periasamy, Sivakumar Hyde, Matthew A. Williams, Julie M. Shi, Pei-Yong Schnell, Matthias J. Bukreyev, Alexander NPJ Vaccines Article The development of effective countermeasures against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the agent responsible for the COVID-19 pandemic, is a priority. We designed and produced ConVac, a replication-competent vesicular stomatitis virus (VSV) vaccine vector that expresses the S1 subunit of SARS-CoV-2 spike protein. We used golden Syrian hamsters as animal models of severe COVID-19 to test the efficacy of the ConVac vaccine. A single vaccine dose elicited high levels of SARS-CoV-2 specific binding and neutralizing antibodies; following intranasal challenge with SARS-CoV-2, animals were protected from weight loss and viral replication in the lungs. No enhanced pathology was observed in vaccinated animals upon challenge, but some inflammation was still detected. The data indicate rapid control of SARS-CoV-2 replication by the S1-based VSV-vectored SARS-CoV-2 ConVac vaccine. Nature Publishing Group UK 2021-07-22 /pmc/articles/PMC8298481/ /pubmed/34294728 http://dx.doi.org/10.1038/s41541-021-00352-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Malherbe, Delphine C.
Kurup, Drishya
Wirblich, Christoph
Ronk, Adam J.
Mire, Chad
Kuzmina, Natalia
Shaik, Noor
Periasamy, Sivakumar
Hyde, Matthew A.
Williams, Julie M.
Shi, Pei-Yong
Schnell, Matthias J.
Bukreyev, Alexander
A single dose of replication-competent VSV-vectored vaccine expressing SARS-CoV-2 S1 protects against virus replication in a hamster model of severe COVID-19
title A single dose of replication-competent VSV-vectored vaccine expressing SARS-CoV-2 S1 protects against virus replication in a hamster model of severe COVID-19
title_full A single dose of replication-competent VSV-vectored vaccine expressing SARS-CoV-2 S1 protects against virus replication in a hamster model of severe COVID-19
title_fullStr A single dose of replication-competent VSV-vectored vaccine expressing SARS-CoV-2 S1 protects against virus replication in a hamster model of severe COVID-19
title_full_unstemmed A single dose of replication-competent VSV-vectored vaccine expressing SARS-CoV-2 S1 protects against virus replication in a hamster model of severe COVID-19
title_short A single dose of replication-competent VSV-vectored vaccine expressing SARS-CoV-2 S1 protects against virus replication in a hamster model of severe COVID-19
title_sort single dose of replication-competent vsv-vectored vaccine expressing sars-cov-2 s1 protects against virus replication in a hamster model of severe covid-19
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298481/
https://www.ncbi.nlm.nih.gov/pubmed/34294728
http://dx.doi.org/10.1038/s41541-021-00352-1
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