Structure of the molecular bushing of the bacterial flagellar motor

The basal body of the bacterial flagellum is a rotary motor that consists of several rings (C, MS and LP) and a rod. The LP ring acts as a bushing supporting the distal rod for its rapid and stable rotation without much friction. Here, we use electron cryomicroscopy to describe the LP ring structure...

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Autores principales: Yamaguchi, Tomoko, Makino, Fumiaki, Miyata, Tomoko, Minamino, Tohru, Kato, Takayuki, Namba, Keiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298488/
https://www.ncbi.nlm.nih.gov/pubmed/34294704
http://dx.doi.org/10.1038/s41467-021-24715-3
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author Yamaguchi, Tomoko
Makino, Fumiaki
Miyata, Tomoko
Minamino, Tohru
Kato, Takayuki
Namba, Keiichi
author_facet Yamaguchi, Tomoko
Makino, Fumiaki
Miyata, Tomoko
Minamino, Tohru
Kato, Takayuki
Namba, Keiichi
author_sort Yamaguchi, Tomoko
collection PubMed
description The basal body of the bacterial flagellum is a rotary motor that consists of several rings (C, MS and LP) and a rod. The LP ring acts as a bushing supporting the distal rod for its rapid and stable rotation without much friction. Here, we use electron cryomicroscopy to describe the LP ring structure around the rod, at 3.5 Å resolution, from Salmonella Typhimurium. The structure shows 26-fold rotational symmetry and intricate intersubunit interactions of each subunit with up to six partners, which explains the structural stability. The inner surface is charged both positively and negatively. Positive charges on the P ring (the part of the LP ring that is embedded within the peptidoglycan layer) presumably play important roles in its initial assembly around the rod with a negatively charged surface.
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spelling pubmed-82984882021-08-12 Structure of the molecular bushing of the bacterial flagellar motor Yamaguchi, Tomoko Makino, Fumiaki Miyata, Tomoko Minamino, Tohru Kato, Takayuki Namba, Keiichi Nat Commun Article The basal body of the bacterial flagellum is a rotary motor that consists of several rings (C, MS and LP) and a rod. The LP ring acts as a bushing supporting the distal rod for its rapid and stable rotation without much friction. Here, we use electron cryomicroscopy to describe the LP ring structure around the rod, at 3.5 Å resolution, from Salmonella Typhimurium. The structure shows 26-fold rotational symmetry and intricate intersubunit interactions of each subunit with up to six partners, which explains the structural stability. The inner surface is charged both positively and negatively. Positive charges on the P ring (the part of the LP ring that is embedded within the peptidoglycan layer) presumably play important roles in its initial assembly around the rod with a negatively charged surface. Nature Publishing Group UK 2021-07-22 /pmc/articles/PMC8298488/ /pubmed/34294704 http://dx.doi.org/10.1038/s41467-021-24715-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yamaguchi, Tomoko
Makino, Fumiaki
Miyata, Tomoko
Minamino, Tohru
Kato, Takayuki
Namba, Keiichi
Structure of the molecular bushing of the bacterial flagellar motor
title Structure of the molecular bushing of the bacterial flagellar motor
title_full Structure of the molecular bushing of the bacterial flagellar motor
title_fullStr Structure of the molecular bushing of the bacterial flagellar motor
title_full_unstemmed Structure of the molecular bushing of the bacterial flagellar motor
title_short Structure of the molecular bushing of the bacterial flagellar motor
title_sort structure of the molecular bushing of the bacterial flagellar motor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298488/
https://www.ncbi.nlm.nih.gov/pubmed/34294704
http://dx.doi.org/10.1038/s41467-021-24715-3
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