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A pancancer overview of FBN1, asprosin and its cognate receptor OR4M1 with detailed expression profiling in ovarian cancer

Ovarian cancer affects >295,000 women worldwide and is the most lethal of gynaecological malignancies. Often diagnosed at a late stage, current research efforts seek to further the molecular understanding of its aetiopathogenesis and the development of novel biomarkers. The present study investig...

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Autores principales: Kerslake, Rachel, Hall, Marcia, Vagnarelli, Paola, Jeyaneethi, Jeyarooban, Randeva, Harpal S., Pados, George, Kyrou, Ioannis, Karteris, Emmanouil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298991/
https://www.ncbi.nlm.nih.gov/pubmed/34386072
http://dx.doi.org/10.3892/ol.2021.12911
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author Kerslake, Rachel
Hall, Marcia
Vagnarelli, Paola
Jeyaneethi, Jeyarooban
Randeva, Harpal S.
Pados, George
Kyrou, Ioannis
Karteris, Emmanouil
author_facet Kerslake, Rachel
Hall, Marcia
Vagnarelli, Paola
Jeyaneethi, Jeyarooban
Randeva, Harpal S.
Pados, George
Kyrou, Ioannis
Karteris, Emmanouil
author_sort Kerslake, Rachel
collection PubMed
description Ovarian cancer affects >295,000 women worldwide and is the most lethal of gynaecological malignancies. Often diagnosed at a late stage, current research efforts seek to further the molecular understanding of its aetiopathogenesis and the development of novel biomarkers. The present study investigated the expression levels of the glucogenic hormone asprosin [encoded by fibrillin-1 (FBN1)], and its cognate receptor, olfactory receptor 4M1 (OR4M1), in ovarian cancer. A blend of in silico open access The Cancer Genome Atlas data, as well as in vitro reverse transcription-quantitative PCR (RT-qPCR), immunohistochemistry and immunofluorescence data were used. RT-qPCR revealed expression levels of OR4M1 and FBN1 in clinical samples and in ovarian cancer cell lines (SKOV-3, PEO1, PEO4 and MDAH-2774), as well as the normal human ovarian surface epithelial cell line (HOSEpiC). Immunohistochemical staining of a tissue microarray was used to identify the expression levels of OR4M1 and asprosin in ovarian cancer samples of varying histological subtype and grade, including clear cell carcinoma, serous ovarian cancer and mucinous adenocarcinoma. Immunofluorescence analysis revealed asprosin expression in SKOV-3 and HOSEpiC cells. These results demonstrated the expression of both asprosin and OR4M1 in normal and malignant human ovarian tissues. This research invokes further investigation to advance the understanding of the role of asprosin and OR4M1 within the ovarian tumour microenvironment.
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spelling pubmed-82989912021-08-11 A pancancer overview of FBN1, asprosin and its cognate receptor OR4M1 with detailed expression profiling in ovarian cancer Kerslake, Rachel Hall, Marcia Vagnarelli, Paola Jeyaneethi, Jeyarooban Randeva, Harpal S. Pados, George Kyrou, Ioannis Karteris, Emmanouil Oncol Lett Articles Ovarian cancer affects >295,000 women worldwide and is the most lethal of gynaecological malignancies. Often diagnosed at a late stage, current research efforts seek to further the molecular understanding of its aetiopathogenesis and the development of novel biomarkers. The present study investigated the expression levels of the glucogenic hormone asprosin [encoded by fibrillin-1 (FBN1)], and its cognate receptor, olfactory receptor 4M1 (OR4M1), in ovarian cancer. A blend of in silico open access The Cancer Genome Atlas data, as well as in vitro reverse transcription-quantitative PCR (RT-qPCR), immunohistochemistry and immunofluorescence data were used. RT-qPCR revealed expression levels of OR4M1 and FBN1 in clinical samples and in ovarian cancer cell lines (SKOV-3, PEO1, PEO4 and MDAH-2774), as well as the normal human ovarian surface epithelial cell line (HOSEpiC). Immunohistochemical staining of a tissue microarray was used to identify the expression levels of OR4M1 and asprosin in ovarian cancer samples of varying histological subtype and grade, including clear cell carcinoma, serous ovarian cancer and mucinous adenocarcinoma. Immunofluorescence analysis revealed asprosin expression in SKOV-3 and HOSEpiC cells. These results demonstrated the expression of both asprosin and OR4M1 in normal and malignant human ovarian tissues. This research invokes further investigation to advance the understanding of the role of asprosin and OR4M1 within the ovarian tumour microenvironment. D.A. Spandidos 2021-09 2021-07-09 /pmc/articles/PMC8298991/ /pubmed/34386072 http://dx.doi.org/10.3892/ol.2021.12911 Text en Copyright: © Kerslake et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Articles
Kerslake, Rachel
Hall, Marcia
Vagnarelli, Paola
Jeyaneethi, Jeyarooban
Randeva, Harpal S.
Pados, George
Kyrou, Ioannis
Karteris, Emmanouil
A pancancer overview of FBN1, asprosin and its cognate receptor OR4M1 with detailed expression profiling in ovarian cancer
title A pancancer overview of FBN1, asprosin and its cognate receptor OR4M1 with detailed expression profiling in ovarian cancer
title_full A pancancer overview of FBN1, asprosin and its cognate receptor OR4M1 with detailed expression profiling in ovarian cancer
title_fullStr A pancancer overview of FBN1, asprosin and its cognate receptor OR4M1 with detailed expression profiling in ovarian cancer
title_full_unstemmed A pancancer overview of FBN1, asprosin and its cognate receptor OR4M1 with detailed expression profiling in ovarian cancer
title_short A pancancer overview of FBN1, asprosin and its cognate receptor OR4M1 with detailed expression profiling in ovarian cancer
title_sort pancancer overview of fbn1, asprosin and its cognate receptor or4m1 with detailed expression profiling in ovarian cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298991/
https://www.ncbi.nlm.nih.gov/pubmed/34386072
http://dx.doi.org/10.3892/ol.2021.12911
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