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Restoration of the ATG5-dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs

Autophagy serves an important role in cancer cell survival and drug resistance. In the present study, the prostate cancer DU145 cell line was used, which lacks autophagy related 5 (ATG5) expression and is defective in induction of ATG5-dependent autophagy. The aim of the study was to examine the eff...

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Autores principales: Peng, Ke, Sun, Aiqin, Zhu, Jun, Gao, Jinyi, Li, Yanlin, Shao, Genbao, Yang, Wannian, Lin, Qiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298997/
https://www.ncbi.nlm.nih.gov/pubmed/34386060
http://dx.doi.org/10.3892/ol.2021.12899
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author Peng, Ke
Sun, Aiqin
Zhu, Jun
Gao, Jinyi
Li, Yanlin
Shao, Genbao
Yang, Wannian
Lin, Qiong
author_facet Peng, Ke
Sun, Aiqin
Zhu, Jun
Gao, Jinyi
Li, Yanlin
Shao, Genbao
Yang, Wannian
Lin, Qiong
author_sort Peng, Ke
collection PubMed
description Autophagy serves an important role in cancer cell survival and drug resistance. In the present study, the prostate cancer DU145 cell line was used, which lacks autophagy related 5 (ATG5) expression and is defective in induction of ATG5-dependent autophagy. The aim of the study was to examine the effects of the restoration of autophagy on cell proliferation and migration, and to assess the cytotoxicity caused by chemotherapeutic drugs, using microscopic, wound-healing, western blot and apoptotic assays. The restoration of the autophagic activity in DU145 cells by the overexpression of ATG5 enhanced the cell proliferation and migration rates. Notably, restoration of the ATG5-dependent autophagy in DU145 cells significantly increased the cytotoxic effects of the chemotherapeutic drugs, docetaxel and valproic acid, and the endoplasmic reticulum stress inducers, brefeldin A, tunicamycin and thapsigargin. The present study provides a novel perspective on the role of ATG5-dependent autophagy in drug resistance and chemotherapy.
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spelling pubmed-82989972021-08-11 Restoration of the ATG5-dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs Peng, Ke Sun, Aiqin Zhu, Jun Gao, Jinyi Li, Yanlin Shao, Genbao Yang, Wannian Lin, Qiong Oncol Lett Articles Autophagy serves an important role in cancer cell survival and drug resistance. In the present study, the prostate cancer DU145 cell line was used, which lacks autophagy related 5 (ATG5) expression and is defective in induction of ATG5-dependent autophagy. The aim of the study was to examine the effects of the restoration of autophagy on cell proliferation and migration, and to assess the cytotoxicity caused by chemotherapeutic drugs, using microscopic, wound-healing, western blot and apoptotic assays. The restoration of the autophagic activity in DU145 cells by the overexpression of ATG5 enhanced the cell proliferation and migration rates. Notably, restoration of the ATG5-dependent autophagy in DU145 cells significantly increased the cytotoxic effects of the chemotherapeutic drugs, docetaxel and valproic acid, and the endoplasmic reticulum stress inducers, brefeldin A, tunicamycin and thapsigargin. The present study provides a novel perspective on the role of ATG5-dependent autophagy in drug resistance and chemotherapy. D.A. Spandidos 2021-09 2021-07-06 /pmc/articles/PMC8298997/ /pubmed/34386060 http://dx.doi.org/10.3892/ol.2021.12899 Text en Copyright: © Peng et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Peng, Ke
Sun, Aiqin
Zhu, Jun
Gao, Jinyi
Li, Yanlin
Shao, Genbao
Yang, Wannian
Lin, Qiong
Restoration of the ATG5-dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs
title Restoration of the ATG5-dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs
title_full Restoration of the ATG5-dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs
title_fullStr Restoration of the ATG5-dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs
title_full_unstemmed Restoration of the ATG5-dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs
title_short Restoration of the ATG5-dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs
title_sort restoration of the atg5-dependent autophagy sensitizes du145 prostate cancer cells to chemotherapeutic drugs
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298997/
https://www.ncbi.nlm.nih.gov/pubmed/34386060
http://dx.doi.org/10.3892/ol.2021.12899
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