Cargando…

Humoral immunity to SARS-CoV-2 and seasonal coronaviruses in children and adults in north-eastern France

BACKGROUND: Children are underrepresented in the COVID-19 pandemic and often experience milder disease than adolescents and adults. Reduced severity is possibly due to recent and more frequent seasonal human coronaviruses (HCoV) infections. We assessed the seroprevalence of SARS-CoV-2 and seasonal H...

Descripción completa

Detalles Bibliográficos
Autores principales: Woudenberg, Tom, Pelleau, Stéphane, Anna, François, Attia, Mikael, Donnadieu, Françoise, Gravet, Alain, Lohmann, Caroline, Seraphin, Hélène, Guiheneuf, Raphaël, Delamare, Catherine, Stefic, Karl, Marlet, Julien, Brochot, Etienne, Castelain, Sandrine, Augereau, Olivier, Sibilia, Jean, Dubos, François, Meddour, Damia, Guen, Christèle Gras-Le, Coste-Burel, Marianne, Imbert-Marcille, Berthe-Marie, Chauvire-Drouard, Anne, Schweitzer, Cyril, Gatin, Amélie, Lomazzi, Sandra, Joulié, Aline, HAAS, Hervé, Cantais, Aymeric, Bertholon, Frederique, Chinazzo-Vigouroux, Marie-France, Abdallah, Mohamed SI, Arowas, Laurence, Charneau, Pierre, Hoen, Bruno, Demeret, Caroline, Werf, Sylvie Van Der, Fontanet, Arnaud, White, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299153/
https://www.ncbi.nlm.nih.gov/pubmed/34304047
http://dx.doi.org/10.1016/j.ebiom.2021.103495
_version_ 1783726209556807680
author Woudenberg, Tom
Pelleau, Stéphane
Anna, François
Attia, Mikael
Donnadieu, Françoise
Gravet, Alain
Lohmann, Caroline
Seraphin, Hélène
Guiheneuf, Raphaël
Delamare, Catherine
Stefic, Karl
Marlet, Julien
Brochot, Etienne
Castelain, Sandrine
Augereau, Olivier
Sibilia, Jean
Dubos, François
Meddour, Damia
Guen, Christèle Gras-Le
Coste-Burel, Marianne
Imbert-Marcille, Berthe-Marie
Chauvire-Drouard, Anne
Schweitzer, Cyril
Gatin, Amélie
Lomazzi, Sandra
Joulié, Aline
HAAS, Hervé
Cantais, Aymeric
Bertholon, Frederique
Chinazzo-Vigouroux, Marie-France
Abdallah, Mohamed SI
Arowas, Laurence
Charneau, Pierre
Hoen, Bruno
Demeret, Caroline
Werf, Sylvie Van Der
Fontanet, Arnaud
White, Michael
author_facet Woudenberg, Tom
Pelleau, Stéphane
Anna, François
Attia, Mikael
Donnadieu, Françoise
Gravet, Alain
Lohmann, Caroline
Seraphin, Hélène
Guiheneuf, Raphaël
Delamare, Catherine
Stefic, Karl
Marlet, Julien
Brochot, Etienne
Castelain, Sandrine
Augereau, Olivier
Sibilia, Jean
Dubos, François
Meddour, Damia
Guen, Christèle Gras-Le
Coste-Burel, Marianne
Imbert-Marcille, Berthe-Marie
Chauvire-Drouard, Anne
Schweitzer, Cyril
Gatin, Amélie
Lomazzi, Sandra
Joulié, Aline
HAAS, Hervé
Cantais, Aymeric
Bertholon, Frederique
Chinazzo-Vigouroux, Marie-France
Abdallah, Mohamed SI
Arowas, Laurence
Charneau, Pierre
Hoen, Bruno
Demeret, Caroline
Werf, Sylvie Van Der
Fontanet, Arnaud
White, Michael
author_sort Woudenberg, Tom
collection PubMed
description BACKGROUND: Children are underrepresented in the COVID-19 pandemic and often experience milder disease than adolescents and adults. Reduced severity is possibly due to recent and more frequent seasonal human coronaviruses (HCoV) infections. We assessed the seroprevalence of SARS-CoV-2 and seasonal HCoV specific antibodies in a large cohort in north-eastern France. METHODS: In this cross-sectional seroprevalence study, serum samples were collected from children and adults requiring hospital admission for non-COVID-19 between February and August 2020. Antibody responses to SARS-CoV-2 and seasonal HCoV (229E, HKU1, NL63, OC43) were assessed using a bead-based multiplex assay, Luciferase-Linked ImmunoSorbent Assay, and a pseudotype neutralisation assay. FINDINGS: In 2,408 individuals, seroprevalence of SARS-CoV-2-specific antibodies was 7-8% with three different immunoassays. Antibody levels to seasonal HCoV increased substantially up to the age of 10. Antibody responses in SARS-CoV-2 seropositive individuals were lowest in adults 18-30 years. In SARS-CoV-2 seronegative individuals, we observed cross-reactivity between antibodies to the four HCoV and SARS-CoV-2 Spike. In contrast to other antibodies to SARS-CoV-2, specific antibodies to sub-unit 2 of Spike (S2) in seronegative samples were highest in children. Upon infection with SARS-CoV-2, antibody levels to Spike of betacoronavirus OC43 increased across the whole age spectrum. No SARS-CoV-2 seropositive individuals with low levels of antibodies to seasonal HCoV were observed. INTERPRETATION: Our findings underline significant cross-reactivity between antibodies to SARS-CoV-2 and seasonal HCoV, but provide no significant evidence for cross-protective immunity to SARS-CoV-2 infection due to a recent seasonal HCoV infection. In particular, across all age groups we did not observe SARS-CoV-2 infected individuals with low levels of antibodies to seasonal HCoV. FUNDING: This work was supported by the « URGENCE COVID-19 » fundraising campaign of Institut Pasteur, by the French Government's Investissement d'Avenir program, Laboratoire d'Excellence Integrative Biology of Emerging Infectious Diseases (Grant No. ANR-10-LABX-62-IBEID), and by the REACTing (Research & Action Emerging Infectious Diseases), and by the RECOVER project funded by the European Union's Horizon 2020 research and innovation programme under grant agreement No. 101003589, and by a grant from LabEx IBEID (ANR-10-LABX-62-IBEID).
format Online
Article
Text
id pubmed-8299153
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-82991532021-07-23 Humoral immunity to SARS-CoV-2 and seasonal coronaviruses in children and adults in north-eastern France Woudenberg, Tom Pelleau, Stéphane Anna, François Attia, Mikael Donnadieu, Françoise Gravet, Alain Lohmann, Caroline Seraphin, Hélène Guiheneuf, Raphaël Delamare, Catherine Stefic, Karl Marlet, Julien Brochot, Etienne Castelain, Sandrine Augereau, Olivier Sibilia, Jean Dubos, François Meddour, Damia Guen, Christèle Gras-Le Coste-Burel, Marianne Imbert-Marcille, Berthe-Marie Chauvire-Drouard, Anne Schweitzer, Cyril Gatin, Amélie Lomazzi, Sandra Joulié, Aline HAAS, Hervé Cantais, Aymeric Bertholon, Frederique Chinazzo-Vigouroux, Marie-France Abdallah, Mohamed SI Arowas, Laurence Charneau, Pierre Hoen, Bruno Demeret, Caroline Werf, Sylvie Van Der Fontanet, Arnaud White, Michael EBioMedicine Research Paper BACKGROUND: Children are underrepresented in the COVID-19 pandemic and often experience milder disease than adolescents and adults. Reduced severity is possibly due to recent and more frequent seasonal human coronaviruses (HCoV) infections. We assessed the seroprevalence of SARS-CoV-2 and seasonal HCoV specific antibodies in a large cohort in north-eastern France. METHODS: In this cross-sectional seroprevalence study, serum samples were collected from children and adults requiring hospital admission for non-COVID-19 between February and August 2020. Antibody responses to SARS-CoV-2 and seasonal HCoV (229E, HKU1, NL63, OC43) were assessed using a bead-based multiplex assay, Luciferase-Linked ImmunoSorbent Assay, and a pseudotype neutralisation assay. FINDINGS: In 2,408 individuals, seroprevalence of SARS-CoV-2-specific antibodies was 7-8% with three different immunoassays. Antibody levels to seasonal HCoV increased substantially up to the age of 10. Antibody responses in SARS-CoV-2 seropositive individuals were lowest in adults 18-30 years. In SARS-CoV-2 seronegative individuals, we observed cross-reactivity between antibodies to the four HCoV and SARS-CoV-2 Spike. In contrast to other antibodies to SARS-CoV-2, specific antibodies to sub-unit 2 of Spike (S2) in seronegative samples were highest in children. Upon infection with SARS-CoV-2, antibody levels to Spike of betacoronavirus OC43 increased across the whole age spectrum. No SARS-CoV-2 seropositive individuals with low levels of antibodies to seasonal HCoV were observed. INTERPRETATION: Our findings underline significant cross-reactivity between antibodies to SARS-CoV-2 and seasonal HCoV, but provide no significant evidence for cross-protective immunity to SARS-CoV-2 infection due to a recent seasonal HCoV infection. In particular, across all age groups we did not observe SARS-CoV-2 infected individuals with low levels of antibodies to seasonal HCoV. FUNDING: This work was supported by the « URGENCE COVID-19 » fundraising campaign of Institut Pasteur, by the French Government's Investissement d'Avenir program, Laboratoire d'Excellence Integrative Biology of Emerging Infectious Diseases (Grant No. ANR-10-LABX-62-IBEID), and by the REACTing (Research & Action Emerging Infectious Diseases), and by the RECOVER project funded by the European Union's Horizon 2020 research and innovation programme under grant agreement No. 101003589, and by a grant from LabEx IBEID (ANR-10-LABX-62-IBEID). Elsevier 2021-07-23 /pmc/articles/PMC8299153/ /pubmed/34304047 http://dx.doi.org/10.1016/j.ebiom.2021.103495 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Paper
Woudenberg, Tom
Pelleau, Stéphane
Anna, François
Attia, Mikael
Donnadieu, Françoise
Gravet, Alain
Lohmann, Caroline
Seraphin, Hélène
Guiheneuf, Raphaël
Delamare, Catherine
Stefic, Karl
Marlet, Julien
Brochot, Etienne
Castelain, Sandrine
Augereau, Olivier
Sibilia, Jean
Dubos, François
Meddour, Damia
Guen, Christèle Gras-Le
Coste-Burel, Marianne
Imbert-Marcille, Berthe-Marie
Chauvire-Drouard, Anne
Schweitzer, Cyril
Gatin, Amélie
Lomazzi, Sandra
Joulié, Aline
HAAS, Hervé
Cantais, Aymeric
Bertholon, Frederique
Chinazzo-Vigouroux, Marie-France
Abdallah, Mohamed SI
Arowas, Laurence
Charneau, Pierre
Hoen, Bruno
Demeret, Caroline
Werf, Sylvie Van Der
Fontanet, Arnaud
White, Michael
Humoral immunity to SARS-CoV-2 and seasonal coronaviruses in children and adults in north-eastern France
title Humoral immunity to SARS-CoV-2 and seasonal coronaviruses in children and adults in north-eastern France
title_full Humoral immunity to SARS-CoV-2 and seasonal coronaviruses in children and adults in north-eastern France
title_fullStr Humoral immunity to SARS-CoV-2 and seasonal coronaviruses in children and adults in north-eastern France
title_full_unstemmed Humoral immunity to SARS-CoV-2 and seasonal coronaviruses in children and adults in north-eastern France
title_short Humoral immunity to SARS-CoV-2 and seasonal coronaviruses in children and adults in north-eastern France
title_sort humoral immunity to sars-cov-2 and seasonal coronaviruses in children and adults in north-eastern france
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299153/
https://www.ncbi.nlm.nih.gov/pubmed/34304047
http://dx.doi.org/10.1016/j.ebiom.2021.103495
work_keys_str_mv AT woudenbergtom humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT pelleaustephane humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT annafrancois humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT attiamikael humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT donnadieufrancoise humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT gravetalain humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT lohmanncaroline humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT seraphinhelene humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT guiheneufraphael humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT delamarecatherine humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT stefickarl humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT marletjulien humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT brochotetienne humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT castelainsandrine humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT augereauolivier humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT sibiliajean humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT dubosfrancois humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT meddourdamia humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT guenchristelegrasle humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT costeburelmarianne humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT imbertmarcilleberthemarie humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT chauviredrouardanne humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT schweitzercyril humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT gatinamelie humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT lomazzisandra humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT jouliealine humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT haasherve humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT cantaisaymeric humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT bertholonfrederique humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT chinazzovigourouxmariefrance humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT abdallahmohamedsi humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT arowaslaurence humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT charneaupierre humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT hoenbruno humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT demeretcaroline humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT werfsylvievander humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT fontanetarnaud humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance
AT whitemichael humoralimmunitytosarscov2andseasonalcoronavirusesinchildrenandadultsinnortheasternfrance