A Standard Scale to Measure Equine Keeper Status and the Effect of Metabolic Tendency on Gut Microbiome Structure

SIMPLE SUMMARY: Horses with different metabolic tendencies are anecdotally referred to as “easy” or “hard” keepers. Easy keepers tend to gain weight easily while hard keepers require extra feed to maintain condition. Both easy and hard keeper horses carry a managerial and financial burden which can be a dissuading factor for horse shoppers. This research uses energy intake/need and body condition to develop a standard Equine Keeper Status Scale (EKSS) for assigning keeper status. The microbiome compositions based on EKSS assignments are then compared to explore microbiome differences based on metabolic tendencies of each group. The EKSS can be used by owners to accurately assess their horses’ metabolic tendencies and make improved feeding decisions to meet their horses’ needs. Understanding microbiome differences between easy, medium and hard keeper horses points to potential microbial roles in these metabolic tendencies. ABSTRACT: Thriftiness in horses has been associated with more efficient nutrient harvesting in digestion, absorption and/or utilization, but the relative contribution of the gut microbiome to host metabolic tendency is not well understood. Recognizing the unreliability of owner reported assignment of keeper status, this research describes a novel tool for calculating whether a horse is an easy (EK) or hard (HK) keeper and then characterizes microbiome differences in these groups. The Equine Keeper Status Scale (EKSS) was developed and validated based on data gathered from 240 horses. Estimates of dietary energy intakes and requirements to achieve the optimal BCS score of 5 were used in EKSS assignments. Sixty percent of owners’ characterizations disagreed with EKSS identified keeper assignments. Equine fecal 16S rRNA profiles (n = 73) revealed differences in α and β diversities and taxa abundances based on EKSS assignments. EK communities had more Planctomycetes and fewer Euryarcheaota, Spirochaetes and Proteobacteria than HK indicating functional differences in nutrient harvesting between groups. Differences in the gut microbiomes of horses based on keeper assignment point to host/microbial interactions that may underlie some differences in metabolic tendency. The EKSS enables robust, repeatable determination of keeper status which can be used by researchers and horse owners.