Cargando…

Single Nucleotide Polymorphisms in Genes Encoding Toll-Like Receptors 7 and 8 and Their Association with Proviral Load of SRLVs in Goats of Polish Carpathian Breed

SIMPLE SUMMARY: The purpose of the study was to identify SNPs in genes encoding TLR7 and TLR8 in goats of Carpathian breed and analyze their association with the SRLVs provirus concentration. A total of 14 SNPs were detected, 6 SNPs in the TLR7 gene locus and 8 SNPs in the TLR8 gene. These SNPs were...

Descripción completa

Detalles Bibliográficos
Autores principales: Olech, Monika, Ropka-Molik, Katarzyna, Szmatoła, Tomasz, Piórkowska, Katarzyna, Kuźmak, Jacek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300119/
https://www.ncbi.nlm.nih.gov/pubmed/34206971
http://dx.doi.org/10.3390/ani11071908
_version_ 1783726399630082048
author Olech, Monika
Ropka-Molik, Katarzyna
Szmatoła, Tomasz
Piórkowska, Katarzyna
Kuźmak, Jacek
author_facet Olech, Monika
Ropka-Molik, Katarzyna
Szmatoła, Tomasz
Piórkowska, Katarzyna
Kuźmak, Jacek
author_sort Olech, Monika
collection PubMed
description SIMPLE SUMMARY: The purpose of the study was to identify SNPs in genes encoding TLR7 and TLR8 in goats of Carpathian breed and analyze their association with the SRLVs provirus concentration. A total of 14 SNPs were detected, 6 SNPs in the TLR7 gene locus and 8 SNPs in the TLR8 gene. These SNPs were located in intron, 3′UTR and 5′UTR regions and within the coding sequences leading to the synonymous mutations. Our results revealed that 9 out 14 identified polymorphisms were associated with the SRLVs proviral concentration. This finding supports a role for genetic variations of TLR7 and TLR8 in SRLVs infection. ABSTRACT: Toll-like receptors (TLRs) 7 and 8 are important in single-stranded viral RNA recognition, so genetic variation of these genes may play a role in SRLVs infection and disease progression. Present study aimed to identify SNPs in genes encoding TLR7 and TLR8 in goats of Carpathian breed and analyze their association with the SRLVs provirus concentration as index of disease progression. A total of 14 SNPs were detected, 6 SNPs in the TLR7 gene locus and 8 SNPs in the TLR8 gene. Nine of the 14 identified polymorphisms, 4 in the TLR7 gene and 5 in TLR8 gene, were significantly associated with the SRLVs proviral concentration. These SNPs were located in 3′UTR, 5′UTR and intron sequences as well as in the coding sequences, but they led to silent changes. Homozygous genotypes of three TLR7 SNPs (synonymous variant 1:50703293, 3′UTR variant 1:50701297 and 5′UTR variant 1:50718645) were observed in goats with lower provirus copy number as well as in seronegative animals. The results obtained in this study suggest that SNPs of TLR7/TLR8 genes may induce differential innate immune response towards SRLVs affecting proviral concentration and thereby disease pathogenesis and progression. These findings support a role for genetic variations of TLR7 and TLR8 in SRLVs infection and warrants further studies on the effect of TLR7/TLR8 polymorphisms on SRLVs infection in different populations.
format Online
Article
Text
id pubmed-8300119
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-83001192021-07-24 Single Nucleotide Polymorphisms in Genes Encoding Toll-Like Receptors 7 and 8 and Their Association with Proviral Load of SRLVs in Goats of Polish Carpathian Breed Olech, Monika Ropka-Molik, Katarzyna Szmatoła, Tomasz Piórkowska, Katarzyna Kuźmak, Jacek Animals (Basel) Article SIMPLE SUMMARY: The purpose of the study was to identify SNPs in genes encoding TLR7 and TLR8 in goats of Carpathian breed and analyze their association with the SRLVs provirus concentration. A total of 14 SNPs were detected, 6 SNPs in the TLR7 gene locus and 8 SNPs in the TLR8 gene. These SNPs were located in intron, 3′UTR and 5′UTR regions and within the coding sequences leading to the synonymous mutations. Our results revealed that 9 out 14 identified polymorphisms were associated with the SRLVs proviral concentration. This finding supports a role for genetic variations of TLR7 and TLR8 in SRLVs infection. ABSTRACT: Toll-like receptors (TLRs) 7 and 8 are important in single-stranded viral RNA recognition, so genetic variation of these genes may play a role in SRLVs infection and disease progression. Present study aimed to identify SNPs in genes encoding TLR7 and TLR8 in goats of Carpathian breed and analyze their association with the SRLVs provirus concentration as index of disease progression. A total of 14 SNPs were detected, 6 SNPs in the TLR7 gene locus and 8 SNPs in the TLR8 gene. Nine of the 14 identified polymorphisms, 4 in the TLR7 gene and 5 in TLR8 gene, were significantly associated with the SRLVs proviral concentration. These SNPs were located in 3′UTR, 5′UTR and intron sequences as well as in the coding sequences, but they led to silent changes. Homozygous genotypes of three TLR7 SNPs (synonymous variant 1:50703293, 3′UTR variant 1:50701297 and 5′UTR variant 1:50718645) were observed in goats with lower provirus copy number as well as in seronegative animals. The results obtained in this study suggest that SNPs of TLR7/TLR8 genes may induce differential innate immune response towards SRLVs affecting proviral concentration and thereby disease pathogenesis and progression. These findings support a role for genetic variations of TLR7 and TLR8 in SRLVs infection and warrants further studies on the effect of TLR7/TLR8 polymorphisms on SRLVs infection in different populations. MDPI 2021-06-26 /pmc/articles/PMC8300119/ /pubmed/34206971 http://dx.doi.org/10.3390/ani11071908 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olech, Monika
Ropka-Molik, Katarzyna
Szmatoła, Tomasz
Piórkowska, Katarzyna
Kuźmak, Jacek
Single Nucleotide Polymorphisms in Genes Encoding Toll-Like Receptors 7 and 8 and Their Association with Proviral Load of SRLVs in Goats of Polish Carpathian Breed
title Single Nucleotide Polymorphisms in Genes Encoding Toll-Like Receptors 7 and 8 and Their Association with Proviral Load of SRLVs in Goats of Polish Carpathian Breed
title_full Single Nucleotide Polymorphisms in Genes Encoding Toll-Like Receptors 7 and 8 and Their Association with Proviral Load of SRLVs in Goats of Polish Carpathian Breed
title_fullStr Single Nucleotide Polymorphisms in Genes Encoding Toll-Like Receptors 7 and 8 and Their Association with Proviral Load of SRLVs in Goats of Polish Carpathian Breed
title_full_unstemmed Single Nucleotide Polymorphisms in Genes Encoding Toll-Like Receptors 7 and 8 and Their Association with Proviral Load of SRLVs in Goats of Polish Carpathian Breed
title_short Single Nucleotide Polymorphisms in Genes Encoding Toll-Like Receptors 7 and 8 and Their Association with Proviral Load of SRLVs in Goats of Polish Carpathian Breed
title_sort single nucleotide polymorphisms in genes encoding toll-like receptors 7 and 8 and their association with proviral load of srlvs in goats of polish carpathian breed
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300119/
https://www.ncbi.nlm.nih.gov/pubmed/34206971
http://dx.doi.org/10.3390/ani11071908
work_keys_str_mv AT olechmonika singlenucleotidepolymorphismsingenesencodingtolllikereceptors7and8andtheirassociationwithproviralloadofsrlvsingoatsofpolishcarpathianbreed
AT ropkamolikkatarzyna singlenucleotidepolymorphismsingenesencodingtolllikereceptors7and8andtheirassociationwithproviralloadofsrlvsingoatsofpolishcarpathianbreed
AT szmatołatomasz singlenucleotidepolymorphismsingenesencodingtolllikereceptors7and8andtheirassociationwithproviralloadofsrlvsingoatsofpolishcarpathianbreed
AT piorkowskakatarzyna singlenucleotidepolymorphismsingenesencodingtolllikereceptors7and8andtheirassociationwithproviralloadofsrlvsingoatsofpolishcarpathianbreed
AT kuzmakjacek singlenucleotidepolymorphismsingenesencodingtolllikereceptors7and8andtheirassociationwithproviralloadofsrlvsingoatsofpolishcarpathianbreed