Porcine Protein Hydrolysates (PEPTEIVA(®)) Promote Growth and Enhance Systemic Immunity in Gilthead Sea Bream (Sparus aurata)

SIMPLE SUMMARY: The development of functional feeds based on additives intended for supporting somatic growth, as well as promoting and modulating the host’s immune response is a promising and reliable strategy in the post-antibiotic era. In this study, we have evaluated porcine plasma protein hydrolysate (PPH), a by-product of the rendering industry, as a functional ingredient in aquafeeds. Thus, a 92-day nutritional trial was conducted to evaluate the inclusion of PPH in gilthead sea bream (Sparus aurata) diets. In particular, the control diet contained 7% fishmeal (48% protein, 17% fat, and 22 MJ kg(−1) gross energy), whereas the PPH was included in the experimental diet at the expense of 5% fish meal. Results indicated that this rendering by-product had a beneficial effect on the growth performance and feed-efficiency parameters, as well as promoted systemic immunity. In addition, no differences in biochemical skin mucus biomarkers were found between both groups. The present study indicated that porcine protein hydrolysate obtained from blood plasma may be considered as a safe and functional ingredient for aquafeeds. ABSTRACT: The effects of porcine plasma protein hydrolysate (PPH) on growth, feed efficiency, and immune responses was evaluated in Sparus aurata. Fish were fed two isoproteic (48% protein), isolipidic (17% fat), and isoenergetic diets (21.7 MJ/kg) diets, one of them containing 5% PPH at the expense of fishmeal. Both diets were tested for 92 days. A significant increase in growth was observed in fish fed the PPH diet in comparison to the control group (182.2 ± 4.4 vs. 173.8 ± 4.1 g), as well as an increase in feed intake without worsening FCR values. An ex vivo assay, with splenocytes incubated with lipopolysaccharide, was conducted to evaluate the cellular immune competence of fish. Genes involved in humoral immunity (lys, IgM), pro- (tnf-α, il-1β), and anti-inflammatory (tgf-β1, il10) cytokines were upregulated in the PPH group in comparison to the control group. The inclusion of PPH in diets enhanced the antibacterial capacity of skin mucus, as the co-culture of selected bacteria (E. coli, V. anguillarum, and P. anguilliseptica) with skin mucus indicated. The present results showed that the PPH in low fishmeal diets (2%) promoted growth and feed efficiency, as well as enhancing the immune response, which indicates that this is a safe and functional ingredient for aquafeeds.