Impact of Type of Parturition on Colostrum Microbiota Composition and Puppy Survival

SIMPLE SUMMARY: It has long been believed that the bacteria present in milk and colostrum were due to contamination during suckling from the oral cavity of the newborn or the skin of the mother. Colostrum and meconium from newborns were considered sterile. In the last decade, human research has provided evidence that bacteria are present in colostrum, milk, placenta, and the intestine of the newborn. The colostrum microbiota appears to change greatly and very rapidly, and in humans it has been found that it can be influenced by the type of parturition. Because information on the colostrum microbiota in dogs is lacking, the objective of our study was to determine whether the type of parturition affects the colostrum microbiota and the growth and survival of puppies in early life. Bacteria isolated from maternal colostrum and puppies meconium were identified by mass spectrometry. The results of this study provide new information on the colostrum microbiome of healthy dams and suggest that the type of parturition influences the bacterial composition of the colostrum microbiota, which may be an important factor in weight gain and survival of puppies in early life. ABSTRACT: The objective of our study was to determine whether the type of parturition affects the microbiota of the colostrum and the growth and survival of the puppies. Seventy-nine newborn puppies were divided into three groups regarding the type of parturition: vaginal delivery (VD), elective caesarean section (EL-CS), and emergency caesarean section (EM-CS). After the birth of the puppies, swabs of meconium were collected from the puppies and colostrum was obtained from the dam. Many aerobic and anaerobic bacteria were isolated and identified by mass spectrometry (MALDI-TOF MS). The colostrum microbiota of VD and EL-CS puppies contained a significantly higher abundance of bacteria belonging to the genera Staphylococcus, Kocuria and Enterococcus compared with EM-CS colostrum samples. The composition of the meconium microbiota of the puppies present at birth was similar to the colostrum microbiota of their mothers. It was also found that puppies without a meconium microbiota at birth gained weight more slowly compared with puppies with a meconium microbiota at birth. The type of parturition influenced the bacterial composition of the microbiota in the colostrum. Future studies are necessary to further define the significance of the observed differences in microbiota composition between EM-CS compared with EL-CS and VD colostrum microbiota.