Intradermal Testing Results in Horses Affected by Mild-Moderate and Severe Equine Asthma

SIMPLE SUMMARY: Equine asthma is a respiratory syndrome sharing several similarities with human asthma and represents the most common cause of chronic coughing in horses. Based on the severity and recurrence of the conditions, it is classified as mild-moderate or severe equine asthma. Although a precise pathogenetic mechanism has not yet been identified, it is generally agreed that environmental allergens behave as triggers of a hypersensitivity response (HR), culminating in asthmatic exacerbations. In human medicine, the skin prick test is considered the gold standard of allergy testing; similarly, in equine medicine, intradermal testing is used to identify hypersensitivities to specific allergens. The present study describes and compares the results of intradermal testing in horses affected by either mild-moderate or severe equine asthma to evaluate the responsiveness of asthmatic horses and to identify the most frequently involved allergens. Type-I HR was shown to play a major role in the pathogenesis of severe equine asthma, while type-IV HR seems to be involved mostly in milder forms. Insects represented the antigens inducing the most frequent and strongest reactions among asthmatic horses, followed by Dermatophagoides spp. and dog epithelium; these allergens should therefore be considered for avoidance strategies and the future development of desensitizing allergen-specific immunotherapy. ABSTRACT: Equine asthma is an inflammatory respiratory disorder, classified as mild-moderate (MEA) and severe (SEA). SEA is characterized by recurrent exacerbations, consisting of dyspnea, coughing and exercise intolerance; MEA causes poor performance, occasional cough and mucus hypersecretion. Although a precise pathogenesis is not completely understood, allergic mechanisms are considered an important pathophysiological feature of equine asthma. In equine medicine, intradermal testing (IDT) is effective in identifying hypersensitivity to specific allergens. However, to date, the studies about IDT in asthmatic horses obtained contradictory results. This study aims to evaluate IDT responses in MEA and SEA horses and to identify the most significant allergens. Thirty-eight asthmatic horses were enrolled and underwent IDT using 50 allergens; reactions were evaluated at 30 min, 4, 24 and 48 h and were assigned a score from 0 to 4. In SEA horses, the most frequent and strongest reactions were observed at 30 min and 4 h, suggesting the involvement of type I hypersensitivity; in MEA horses, also type IV hypersensitivity seemed to play a major role. Insects, Dermatophagoides spp. and dog epithelium induced in MEA and SEA horses the most significant hypersensitivity responses and could therefore be considered as the main allergenic antigens in our geographic area.